Membrane disruption and enzyme inhibition by naturally-occurring and modified chacotriose-containing Solanum steroidal glycoalkaloids

Roddick, James G.; Weissenberg, Martin; Leonard, Anna L.

doi:10.1016/s0031-9422(00)00420-9

Cited by 89 publications

(85 citation statements)

References 36 publications

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“…According to Cherkaoui et al, (2001), this work proves that, among the plants biosynthesizing steroidal glycoalkaloids, the main aglycone is solasodine, and S. leucocarpum is not the exception to this generalization. In addition, Roddick et al, (2001) pointed out the role of glycoalkaloid in plants as defense molecules against insect pests and/or microbial pathogens. In fact, the action of these compounds against fungal diseases is well documented, although many plant secondary metabolites are known to have antibacterial activities (Amir & Kumar, 2004).…”

Section: Spectroscopic Interpretation Of Steroidal Compounds Isolatedmentioning

confidence: 99%

Biological activities of steroidal alkaloids isolated fromSolanum leucocarpum

Niño¹,

Correa²,

Mosquera³

2009

Pharmaceutical Biology

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Section: Spectroscopic Interpretation Of Steroidal Compounds Isolatedmentioning

confidence: 99%

Biological activities of steroidal alkaloids isolated fromSolanum leucocarpum

Niño¹,

Correa²,

Mosquera³

2009

Pharmaceutical Biology

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“…Another example of a true "viability only" compound is α-Solanine, which had class 1a (mostly) or 2a curves (IC50 >10 μM) in all viability assays and was class 4 in all caspase assays. This compound is known to inhibit cholinesterases (24) and to disrupt membranes (25). Thus, when interpreting these results, reasons for negative findings other than the compound not activating the caspases, e.g.…”

Section: Comparison Of Viability and Caspase-3/7 Assaysmentioning

confidence: 99%

Characterization of Diversity in Toxicity Mechanism Using in Vitro Cytotoxicity Assays in Quantitative High Throughput Screening

Huang

Southall

Cho

et al. 2008

Chem. Res. Toxicol.

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Assessing the potential health risks of environmental chemical compounds is an expensive undertaking which has motivated the development of new alternatives to traditional in vivo toxicological testing. One approach is to stage the evaluation, beginning with less expensive and higher throughput in vitro testing before progressing to more definitive trials. In vitro testing can be used to generate a hypothesis about a compound's mechanism of action, which can then be used to design an appropriate in vivo experiment. Here we begin to address the question of how to design such a battery of in vitro cell-based assays by combining data from two different types of assays, cell viability and caspase activation, with the aim of elucidating mechanism of action. Because caspase activation is a transient event during apoptosis, it is not possible to design a single end-point assay protocol that would identify all instances of compound-induced caspase activation. Nevertheless, useful information about compound mechanism of action can be obtained from these assays in combination with cell viability data. Unsupervised clustering in combination with Dunn's cluster validity index is a robust method for identifying mechanisms of action without requiring any a priori knowledge about mechanisms of toxicity. The performance of this clustering method is evaluated by comparing the clustering results against literature annotations of compound mechanisms.

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“…Interest in these compounds has mainly been focused on their use as a starting material for the industrial synthesis of steroidal hormones (Roddick, 1986). The effects of these compounds mainly consist of membrane disruption and acetylcholinesterase inhibition (Roddick et al, 2001). In addition, solasodine has been known to possess anti-inflammatory activity (Lewis, 1989).…”

Section: Discussionmentioning

confidence: 99%

Anti‐inﬂammatory effect of Solanum lycocarpum fruits

Vieira

Ferreira

Matos

et al. 2003

Phytotherapy Research

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The croton oil-induced mouse ear oedema test, acetic acid-induced abdominal writhing, and carrageenan-induced peritonitis were used to study the anti-inflammatory effects of the crude ethanol extract and its alkaloid fraction from Solanum lycocarpum fruits. The alkaloid fraction induced a dose-dependent reduction in ear oedema formation and leukocyte migration, suggesting that S. lycocarpum fruits may contain steroidal alkaloids accounting for the anti-inflammatory effect of the crude ethanol extract.

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Membrane disruption and enzyme inhibition by naturally-occurring and modified chacotriose-containing Solanum steroidal glycoalkaloids

Cited by 89 publications

References 36 publications

Biological activities of steroidal alkaloids isolated fromSolanum leucocarpum

Biological activities of steroidal alkaloids isolated fromSolanum leucocarpum

Characterization of Diversity in Toxicity Mechanism Using in Vitro Cytotoxicity Assays in Quantitative High Throughput Screening

Anti‐inﬂammatory effect of Solanum lycocarpum fruits

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