Membrane–drug interactions studied using model membrane systems

Knobloch, Jacqueline J.; Suhendro, Daniel K.; Zieleniecki, Julius L.; Shapter, Joseph G.; Köper, Ingo

doi:10.1016/j.sjbs.2015.03.007

Cited by 69 publications

(48 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Taking into account the positive correlation between peptide helicity and bilayer-disturbing potency [43][44][45], the chiroptical data predict that the INH conjugates bind to lipid membranes with greater affinity compared to the native peptides. Based on the measure of the conjugation-induced spectroscopic changes (Figure 3), an estimation of the relative membrane affinity increment can be proposed (in decreasing order): INH-Transportan, INH-Magainin II, and INH-Buforin II (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Importantly, this prediction is in good correlation with the membrane affinity enhancement of these conjugates displayed in Figure 1.…”

Section: Circular Dichroism Spectroscopic Evaluation Of the Secondarymentioning

confidence: 70%

“…Moreover, the incorporation of mycolic acid molecules with long alkyl chains into the lipid layer changes the molecular order and reduces the regular close packing of the DPPC molecules, allowing the interaction with and penetration of the peptides. According to the experimental findings, the membrane affinity significantly increased in all cases except for INH-OT20 and INH-Buforin II (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). INH-Transportan and INH-CM15 have the highest interactions with the DPPC+mycolic acid mixed lipid monolayer.…”

Section: Interaction Of Peptides and Inh-peptide Conjugates With Lipimentioning

confidence: 88%

“…In experimental model systems, mammalian membranes often mimicked by dipalmitoylphosphatidylcholine (DPPC). This lipid is chosen to represent the most abundant head groups in natural membranes [15]. Chimote et al used Langmuir monolayers to evaluate the effect of mycolic acid and cord factor (alone and as mixtures) on the surface properties of DPPC layer [7].…”

Section: Introductionmentioning

confidence: 99%

“…Penetratin [23] and Transportan [24] are among the most-studied CPPs and have the ability to translocate the plasma membrane, delivering drugs to the cytoplasm. The antimicrobial peptide Buforin II (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21) [25] and Magainin II [26] were isolated from amphibians. Dhvar4 is a synthetic derivative of Histatin, which is a salivary histidine-rich cationic AMP found in the human parotid secretion [27], Crot (1)(2)(3)(4)(5)(6)(7)(8)(9)(38)(39)(40)(41)(42) [28] and CM15 [29], which are hybrid AMPs composed of venom peptides.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Drug Conjugation Induced Modulation of Structural and Membrane Interaction Features of Cationic Cell-Permeable Peptides

Pári

Horváti

Bősze

et al. 2020

IJMS

View full text Add to dashboard Cite

Cell-penetrating peptides might have great potential for enhancing the therapeutic effect of drug molecules against such dangerous pathogens as Mycobacterium tuberculosis (Mtb), which causes a major health problem worldwide. A set of cationic cell-penetration peptides with various hydrophobicity were selected and synthesized as drug carrier of isoniazid (INH), a first-line antibacterial agent against tuberculosis. Molecular interactions between the peptides and their INH-conjugates with cell-membrane-forming lipid layers composed of DPPC and mycolic acid (a characteristic component of Mtb cell wall) were evaluated, using the Langmuir balance technique. Secondary structure of the INH conjugates was analyzed and compared to that of the native peptides by circular dichroism spectroscopic experiments performed in aqueous and membrane mimetic environment. A correlation was found between the conjugation induced conformational and membrane affinity changes of the INH–peptide conjugates. The degree and mode of interaction were also characterized by AFM imaging of penetrated lipid layers. In vitro biological evaluation was performed with Penetratin and Transportan conjugates. Results showed similar internalization rate into EBC-1 human squamous cell carcinoma, but markedly different subcellular localization and activity on intracellular Mtb.

show abstract

Section: Circular Dichroism Spectroscopic Evaluation Of the Secondarymentioning

confidence: 70%

Section: Interaction Of Peptides and Inh-peptide Conjugates With Lipimentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Drug Conjugation Induced Modulation of Structural and Membrane Interaction Features of Cationic Cell-Permeable Peptides

Pári

Horváti

Bősze

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…There have only been a small number of studies on interactions between PAMAM dendrimers and Langmuir-monolayer models. However, monolayers have been widely used to explore interactions with other nanoparticles, including gold [101] and silica [102] as well as pharmaceuticals [103].…”

Section: Interactions Between Pamam Dendrimers and Langmuir Monolayersmentioning

confidence: 99%

PAMAM dendrimer - cell membrane interactions

Fox

Richardson

Briscoe

2018

Advances in Colloid and Interface Science

191

135

View full text Add to dashboard Cite

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. 2018 PAMAM dendrimers have been conjectured for a wide range of biomedical applications due to their tuneable physicochemical properties. However, their application has been hindered by uncertainties in their cytotoxicity, which is influenced by dendrimer generation (i.e. size and surface group density), surface chemistry, and dosage, as well as cell specificity. In this review, biomedical applications of polyamidoamine (PAMAM) dendrimers and some related cytotoxicity studies are first outlined. Alongside these in vitro experiments, lipid membranes such as supported lipid bilayers (SLBs), liposomes, and Langmuir monolayers have been used as cell membrane models to study PAMAM dendrimer-membrane interactions. Related experimental and theoretical studies are summarized, and the physical insights from these studies are discussed to shed light on the fundamental understanding of PAMAM dendrimer-cell membrane interactions. We conclude with a summary of some questions that call for further investigations.a b s t r a c t a r t i c l e i n f o Available online 27 June

show abstract

Lipid Microfluidic Biomimetic Models for Drug Screening: A Comprehensive Review

Fernandes,

Cardoso,

Lanceros‐Méndez

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Developing new drugs is a complex, time‐consuming, and expensive process, essential to identify potential pharmacokinetic issues in the early stages to avoid investment in nonpromising candidates. Nearly half of all drug targets and key drug‐metabolizing enzymes are found in intracellular environments, compartmentalized by semipermeable barriers, the cell membranes. Inspired by their lipidic bilayer structure, lipid‐based biomimetic platforms are being developed to understand the biochemical and biophysical processes at the cellular membrane level. Considering the pharmaceutical industry's demands for efficient in vitro high throughput screening tools, microfluidic technology emerges as a promising solution to address challenges in lipid biomimetic models for screening applications. This involves the transformation of commonly used lipid‐based biomimetic models, like supported lipid bilayers and lipid vesicles, to miniaturized approachs and their evolution into a new generation of bilayer models. These include pore‐suspended and free‐standing lipid bilayers, black lipid membranes, and droplet interface bilayers. This review provides an overview of both generations of lipid biomimetic models used in drug‐membrane screening applications. Moreover, it delves into the intricacies of their production through microfluidic approaches and examines their screening applications in drug‐membrane interaction. The review concludes with a critical analysis of potential future directions in this rapidly evolving field.

show abstract

Membrane–drug interactions studied using model membrane systems

Cited by 69 publications

References 33 publications

Drug Conjugation Induced Modulation of Structural and Membrane Interaction Features of Cationic Cell-Permeable Peptides

Drug Conjugation Induced Modulation of Structural and Membrane Interaction Features of Cationic Cell-Permeable Peptides

PAMAM dendrimer - cell membrane interactions

Lipid Microfluidic Biomimetic Models for Drug Screening: A Comprehensive Review

Contact Info

Product

Resources

About