Membrane dynamics in human leukemia and lymphoma cells

Billard, Christian; Tuy, Françoise Phan Dinh; Rosenfeld, C.; Tapiero, Haïm

doi:10.1016/0014-5793(83)80584-5

Cited by 6 publications

References 21 publications

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Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

Cao-Hoang

Marechal

Lê-Thanh

et al. 2008

Biotechnology Journal

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Many fluorescent techniques are employed to evaluate the viability and activity of microbial cells used in biotechnology. These techniques are sometimes complex and the interpretation of results opened to misunderstanding. Moreover, new developments are constantly proposed especially concerning a more accurate evaluation of the state of the cells including eukaryotic microorganisms. This paper aims at presenting to biotechnologists unfamiliar with fluorescence the principles of these methods and the related possible pitfalls. It focuses on probes of the physical (integrity and fluidity) and energetical (intracellular pH and membrane potential) state of the cell membrane (bacterial and yeast cells) and presents also other probes (nucleic acids, respiration...) and new technical trends. The specificities of Gram-negative bacterial cells are also discussed.

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Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

Cao-Hoang

Marechal

Lê-Thanh

et al. 2008

Biotechnology Journal

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Fluorescence Depolarization Techniques in Materials Science

Birch

Karolin

Standardization and Quality Assurance in Fluorescence Measurements I

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Cytoplasmic membrane fluidity measurements on intact living cells ofBacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene

Svobodová

Svoboda

1988

Folia Microbiol

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A method for determination of membrane fluidity (microviscosity) in Bacillus subtilis cytoplasmic membrane under in vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbance A as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).

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Membrane dynamics in human leukemia and lymphoma cells

Cited by 6 publications

References 21 publications

Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

Fluorescence Depolarization Techniques in Materials Science

Cytoplasmic membrane fluidity measurements on intact living cells ofBacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene

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