Membrane-entrapped microperoxidase as a ‘solid-state’ promoter in the electrochemistry of soluble metalloproteins

Abstract

Immobilization of biological systems in solid matrices is presently of great interest, in view of the many potential advantages associated with both the higher stability of the immobilized macromolecules and the potential utilization for biotechnology. In the present paper the electrochemical behaviour of the undecapeptide from cytochrome c (called microperoxidase) tightly entrapped in cellulose triacetate membrane is reported; its utilization as ‘solid-state’ promoter in the electrochemistry of soluble metalloproteins is presented. The results obtained indicate that: (i) membrane-entrapped microperoxidase undergoes rapid reversible electron transfer at a glassy carbon electrode; (ii) the electrochemical process is diffusion-controlled; (iii) entrapped microperoxidase acts as ‘solid-state’ promoter in the electrochemistry of soluble cytochrome c and of azurin.

Unmediated heterogeneous electron transfer reaction of ascorbate oxidase and laccase at a gold electrode

“…The immobilized system should satisfy some fundamental requirements, the first of which is to provide the ET pathway typical of the protein in the native conformation. The incorporation of biological systems within films of different nature and their deposition on solid electrodes has already been described [10][11][12][13]. In this paper we report the Abbreviations used : AO, ascorbate oxidase ; DC, direct current ; ET, electron transfer ; T2D, type 2 Cu-depleted ; TBMPC, tributylmethyl phosphonium chloride.…”

Can Graphene Bilayers Be the Membrane Mimetic Materials?

“…This approach was intensively developed in the "era of the solid state and semiconductor physics" (until the 1990-th) before the period when the progress in molecular biology and the emergence of nanoscience allowed to understand the mechanism of electron transfer in biological systems in details. At the end of the 1980-th the words "solid state" in description of the operation mechanism of the electron transfer system elements (such as peroxidases -promoters in the soluble NANOSYSTEMS GRADOV OLEG V. metalloprotein electrochemistry [57]) were used with the quotation marks (unlike the promoters for bacteria cultivation on solid media which do not require quotation marks [58,59]). Therefore it can be assumed that the choice of the biomimetic material for the biomembrane modeling and the choice of the corresponding membrane models at that period were in accordance with the "scientific fashion" similar to the rapid shift of the synthetic membrane design towards the nanomaterial science as a result of the development of nanotechnology [60].…”

Electrochemistry of surface wired redox protein: Axial ligation and control of redox potential

“…The effect of axial coordination on the redox properties of MP-8 has been studied by taking both MP-8 and the exogeneous ligands in homogeneous solution [8][9][10][11][12]. Burnori and co-workers investigated the electrochemistry of MP using membranes, bare and gold plated reticulated vitreous carbon thin film [13][14][15]. The electrochemical and electrocatalytic behavior of surface immobilized MP-11 have been investigated by various groups [16][17][18][19].…”