Membrane filtration of dairy products for microbiological analysis

Peterkin, Pearl I.; Sharpe, A. N.

doi:10.1128/aem.39.6.1138-1143.1980

Cited by 34 publications

(13 citation statements)

References 12 publications

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“…The proteins are much smaller in size than both the microorganisms and the 0.2 lm pores in the membranes, but these proteins must still be removed or modified to avoid fouling of the microfiltration membrane and loss of flux. [12][13][14] Previous reports have demonstrated the value of enzymes in reducing membrane fouling and accelerating filtration and microfiltration of fruit juices. More recently, the use of endopeptidase for chicken extract has also been reported (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

Vibbert

Luo

et al. 2015

Biotechnology Progress

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Microfiltration of chicken extracts has the potential to significantly decrease the time required to detect Salmonella, as long as the extract can be efficiently filtered and the pathogenic microorganisms kept in a viable state during this process. We present conditions that enable microfiltration by adding endopeptidase from Bacillus amyloliquefaciens to chicken extracts or chicken rinse, prior to microfiltration with fluid flow on both retentate and permeate sides of 0.2 μm cutoff polysulfone and polyethersulfone hollow fiber membranes. After treatment with this protease, the distribution of micron, submicron, and nanometer particles in chicken extracts changes so that the size of the remaining particles corresponds to 0.4-1 μm. Together with alteration of dissolved proteins, this change helps to explain how membrane fouling might be minimized because the potential foulants are significantly smaller or larger than the membrane pore size. At the same time, we found that the presence of protein protects Salmonella from protease action, thus maintaining cell viability. Concentration and recovery of 1-10 CFU Salmonella/mL from 400 mL chicken rinse is possible in less than 4 h, with the microfiltration step requiring less than 25 min at fluxes of 0.028-0.32 mL/cm(2) min. The entire procedure-from sample processing to detection by polymerase chain reaction-is completed in 8 h.

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Section: Introductionmentioning

confidence: 99%

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

Vibbert

Luo

et al. 2015

Biotechnology Progress

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“…Except with precomminuted foods, Stomacher-type processors yield considerably less debris than a traditional blender. This has been found advantageous in techniques based on membrane filtration of foods (Peterkin andSharpe 1980, 1981), electric impedance (Eden and Eden 1984) and dye reduction (Holley ef al. 1977).…”

Section: Introductionmentioning

confidence: 99%

COMPARISON OF THE PULSIFIER AND THE STOMACHER FOR RECOVERING MICROORGANISMS IN VEGETABLES¹

Jitareerat

Fung

2003

Rapid Methods Automation Mic

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Treatment of 30 different vegetables by the Pulsifier (30 s) and the Stomacher (60 s) showed that total viable cell counts and coliform counts were almost identical with less than 0.2 Log CFU/g difference and a ratio of ca. 1.00 between the two methods. However, the diluents from Pulsified samples were much clearer (Optical Density of 0.052), with less total soluble solids (0.30%) and slightly higher pH (6.56) than Stomached samples, which had more turbid liquid (Optical density of 0.237), with more total solids (0.55%) and lower pH (6.35). These data indicated that the Pulsifier provided equivalent viable cell count and coliform counts compared with the Stomacher from the 30 vegetables studied, but at the same time provided clearer liquid with less total soluble solid. This is advantageous for further microbiological manipulations such as Polymerase Chain Reaction, ATP evaluation, and immunological testing.

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“…Neither trypsin (1%) nor Tween 80 (1 %) alone or in combination adversely affected the viability of the Salmonella serotypes when incubated with pure cultures of the organisms in buffered peptone water, for 45min at 37°C. The non-toxic effects of these agents on microorganisms, including Salmonella, have been reported before (Peterkin & Sharpe 1980;DAoust et al, 1982).…”

Section: E N Z Y M E -S U R F a L ' T A H T T R T A I M T N Tmentioning

confidence: 99%

“…This organism should be absent in 25g of sample when assayed by a prescribed method (Habraken et ul.,1'986). Standard methods for isolating Salmonella from milk powders follow successive steps of pre-enrichment, selective enrichment, plating and confirmation, giving results in 3-4 days (Poelma et al 1984). Apart from the time-delay., these methods are inconvenient and generate only presence or absence data.…”

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A rapid method for enumerating Salmonella in milk powders

Fleet

Karalis

Hawa

et al. 1991

Lett Appl Microbiol

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A one-day procedure for enumerating Salmonella in milk powder is described. Milk powder suspension was treated with a mixture of trypsin and Tween 80, then centrifuged to give a sediment of microbial cells that was plated on the xylose lysine desoxycholate agar for the direct enumeration of Salmonella. Known populations (1-200 cfu/25 g of sample) of salmonellas inoculated into milk powder suspensions were recovered with almost 100% efficiency in each of 152 trials of the method, covering 17 different serotypes

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Membrane filtration of dairy products for microbiological analysis

Cited by 34 publications

References 12 publications

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

COMPARISON OF THE PULSIFIER AND THE STOMACHER FOR RECOVERING MICROORGANISMS IN VEGETABLES¹

A rapid method for enumerating Salmonella in milk powders

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Membrane filtration of dairy products for microbiological analysis

Cited by 34 publications

References 12 publications

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

Accelerating sample preparation through enzyme‐assisted microfiltration of Salmonella in chicken extract

COMPARISON OF THE PULSIFIER AND THE STOMACHER FOR RECOVERING MICROORGANISMS IN VEGETABLES1

A rapid method for enumerating Salmonella in milk powders

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COMPARISON OF THE PULSIFIER AND THE STOMACHER FOR RECOVERING MICROORGANISMS IN VEGETABLES¹