Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

Force, André; Grizard, G.; Giraud, M; Motta, Claude; Sion, Benoı̂t; Boucher, D

doi:10.1046/j.1365-2605.2001.00309.x

Cited by 32 publications

(14 citation statements)

References 42 publications

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“…Epididymosomes and sperm lipids were extracted by the Folch method with modifications [28]. Cholesterol was assayed using Lieberman-Burchard reagent [29], and total phospholipid concentrations were determined by measuring the amount of inorganic phosphorus [30].…”

Section: Lipid Analysesmentioning

confidence: 99%

“…Phospholipid classes were separated by high-performance, thin-layer chromatography (HPTLC; 10 3 10 cm; Merck) with methyl acetate, propan-2-ol, chloroform, methanol, and 0.25% (w/v) aqueous KCl (25:25:25:10:9, v/v) [28,31]. Lipid-containing regions of the chromatogram were visualized by treatment with a 10% (w/v) CuSO 4 and 8% (v/v) H 3 PO 4 solution and heated at 1808C.…”

Section: Lipid Analysesmentioning

confidence: 99%

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Lipid Remodeling of Murine Epididymosomes and Spermatozoa During Epididymal Maturation1

Rejraji

Sion²,

Prensier

et al. 2006

Biology of Reproduction

168

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We have isolated vesicular structures from mouse epididymal fluid, referred to as epididymosomes. Epididymosomes have a roughly spherical aspect and a bilayer membrane, and they are heterogeneous in size and content. They originate from the epididymal epithelium, notably from the caput region, and are emitted in the epididymal lumen by way of apocrine secretion. We characterized their membranous lipid profiles in caput and cauda epididymidal fluid samples and found that epididymosomes were particularly rich in sphingomyelin (SM) and arachidonic acid. The proportion of SM increased markedly during epididymal transit and represented half the total phospholipids in cauda epididymidal epididymosomes. The cholesterol:phospholipid ratio increased from 0.26 in the caput to 0.48 in the cauda epididymidis. Measures of epididymosomal membrane anisotropy revealed that epididymosomes became more rigid during epididymal transit, in agreement with their lipid composition. In addition, we have characterized the membrane lipid pattern of murine epididymal spermatozoa during their maturation. Here, we have shown that mouse epididymal spermatozoa were distinguished by high percentages of SM and polyunsaturated membranous fatty acids (PUFAs), principally represented by arachidonic, docosapentanoic, and docosahexanoic acids. Both SM and PUFA increased throughout the epididymal tract. In particular, we observed a threefold rise in the ratio of docosapentanoic acid. Epididymal spermatozoa had a constant cholesterol:phospholipid ratio (average, 0.30) during epididymal transit. These data suggest that in contrast with epididymosomes, spermatozoal membranes seem to become more fluid during epididymal maturation.

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Section: Lipid Analysesmentioning

confidence: 99%

Section: Lipid Analysesmentioning

confidence: 99%

Lipid Remodeling of Murine Epididymosomes and Spermatozoa During Epididymal Maturation1

Rejraji

Sion²,

Prensier

et al. 2006

Biology of Reproduction

168

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“…, 1991). Abnormal spermatozoa, however, appear to have membrane defects that interfere with their ability to undergo capacitation and acquire fertilizing ability (Force et al. , 2001; Ambrosini et al.…”

Section: Discussionmentioning

confidence: 99%

Capacitation‐associated changes in membrane fluidity in asthenozoospermic human spermatozoa

Buffone¹,

Doncel

Calamera³

et al. 2009

Int J of Andrology

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The fertilizing potential of human spermatozoa relies on their ability to capacitate as they travel through the female reproductive tract. During this process, cholesterol is released from the plasma membrane, altering its architecture and dynamics. Using ISolate gradients, we obtained high (L90)- and low (L45)-quality spermatozoa from asthenozoospermic human semen samples. We tested the hypothesis that the lower fertilizing ability of asthenozoospermic L90 cells could be related to a lower ability to increase their membrane fluidity during capacitation. We assessed two sets of fluorescent probes: (i) DPH, TMA-DPH and PA-DPH which senses the hydrophobic core, cytosolic and exofacial leaflets of the bilayer, respectively and (ii) Laurdan, sensitive to the amount of water molecules intercalated between lipid moieties of the membrane (membrane hydration). Before capacitation, membrane fluidity of asthenozoospermic sperm populations was similar to the corresponding fractions of normozoospermic cells when evaluated with DPH, TMA-DPH or PA-DPH. Asthenozoospermic whole samples displayed lower plasma membrane hydration than normozoospermic cells as evidenced with Laurdan. After capacitation, asthenozoospermic L45 and L90 cells failed to increase their membrane fluidity in opposition to normozoospermic cells. Interestingly, membrane hydration significantly correlated with the main sperm motion parameters analysed, being a low membrane hydration associated with poor sperm movement. These results show that low-motility spermatozoa are unable to respond to capacitation with the necessary changes in membrane fluidity. This defect in sperm plasma membrane rheology may be responsible for their poor functional quality and low fertilizing ability.

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“…The supernatant corresponding to seminal fluid was centrifuged (15 min, 10,000 g, 4°C) to remove cell debris. Lipids present in spermatozoa and in seminal fluid were extracted following a modified Folch procedure [42]. The phospholipids and sterols were separated by high performance thin layer chromatography (HPTLC) using a sequential development system as described in a prior study [43].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of atorvastatin efficacy and toxicity on spermatozoa, accessory glands and gonadal hormones of healthy men: a pilot prospective clinical trial

Pons-Rejraji

Brugnon

Sion

et al. 2014

Reprod Biol Endocrinol

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BackgroundRecommendations for cardiovascular disease prevention advocate lowering both cholesterol and low-density lipoprotein cholesterol systemic levels, notably by statin intake. However, statins are the subject of questions concerning their impact on male fertility. This study aimed to evaluate, by a prospective pilot assay, the efficacy and the toxicity of a decrease of cholesterol blood levels, induced by atorvastatin on semen quality and sexual hormone levels of healthy, normocholesterolaemic and normozoospermic men.MethodsAtorvastatin (10 mg daily) was administrated orally during 5 months to 17 men with normal plasma lipid and standard semen parameters. Spermatozoa parameters, accessory gland markers, semen lipid levels and blood levels of gonadal hormones were assayed before statin intake, during the treatment, and 3 months after its withdrawal.ResultsAtorvastatin treatment significantly decreased circulating low-density lipoprotein cholesterol (LDL-C) and total cholesterol concentrations by 42% and 24% (p < 0.0001) respectively, and reached the efficacy objective of the protocol. During atorvastatin therapy and/or 3 months after its withdrawal numerous semen parameters were significantly modified, such as total number of spermatozoa (-31%, p < 0.05), vitality (-9.5%, p < 0.05), total motility (+7.5%, p < 0.05), morphology (head, neck and midpiece abnormalities, p < 0.05), and the kinetics of acrosome reaction (p < 0.05). Seminal concentrations of acid phosphatases (p < 0.01), α-glucosidase (p < 0.05) and L-carnitine (p < 0.05) were also decreased during the therapy, indicating an alteration of prostatic and epididymal functions. Moreover, we measured at least one altered semen parameter in 35% of the subjects during atorvastatin treatment, and in 65% of the subjects after withdrawal, which led us to consider that atorvastatin is unsafe in the context of our study.ConclusionsOur results show for the first time that atorvastatin significantly affects the sperm parameters and the seminal fluid composition of healthy men.Trial registrationClinicalTrials.gov: NCT02094313.

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Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

Cited by 32 publications

References 42 publications

Lipid Remodeling of Murine Epididymosomes and Spermatozoa During Epididymal Maturation1

Lipid Remodeling of Murine Epididymosomes and Spermatozoa During Epididymal Maturation1

Capacitation‐associated changes in membrane fluidity in asthenozoospermic human spermatozoa

Evaluation of atorvastatin efficacy and toxicity on spermatozoa, accessory glands and gonadal hormones of healthy men: a pilot prospective clinical trial

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