Membrane fluidity of stressed cells of Oenococcus oeni

Tourdot-Maréchal, Raphaëlle; Gaboriau, David; Beney, Laurent; Diviès, Charles

doi:10.1016/s0168-1605(00)00202-6

Cited by 64 publications

(52 citation statements)

References 10 publications

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“…In another study, Saccharomyces cerevisiae with increased membrane fluidity also showed decreased heat resistance while ethanol resistance increased (29,30). It was also reported that when Oenococcus oeni was heat or acid stressed, the membrane fluidity increased in contrast to ethanol stress, which decreased membrane fluidity (44). These reports confirm our results, which showed that L. johnsonii NCC 533 supplemented with unsaturated fatty acids had increased sensitivity to heat and acid stress.…”

Section: Discussionsupporting

confidence: 92%

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Müller

Ross

Sybesma

et al. 2011

Appl Environ Microbiol

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The aim of this study was to investigate the influence of supplementing growth medium with unsaturated fatty acids on the technical properties of the probiotic strain Lactobacillus johnsonii NCC 533, such as heat and acid tolerance, and inhibition of Salmonella enterica serovar Typhimurium infection. Our results showed that the membrane composition and morphology of L. johnsonii NCC 533 were significantly changed by supplementing a minimal Lactobacillus medium with oleic, linoleic, and linolenic acids. The ratio of saturated to unsaturated plus cyclic fatty acids in the bacterial membrane decreased by almost 2-fold when minimal medium was supplemented with unsaturated fatty acids (10 g/ml). The subsequent acid and heat tolerance of L. johnsonii decreased by 6-and 20-fold when the strain was grown in the presence of linoleic and linolenic acids, respectively, compared with growth in oleic acid (all at 10 g/ml). Following acid exposure, significantly higher (P < 0.05) oleic acid content was detected in the membrane when growth medium was supplemented with linoleic or linolenic acid, indicating that saturation of the membrane fatty acids occurred during acid stress. Cell integrity was determined in real time during stressed conditions using a fluorescent viability kit in combination with flow cytometric analysis. Following heat shock (at 62.5°C for 5 min), L. johnsonii was unable to form colonies; however, 60% of the bacteria showed no cell integrity loss, which could indicate that the elevated heat inactivated vital processes within the cell, rendering it incapable of replication. Furthermore, L. johnsonii grown in fatty acid-enriched minimal medium had different adhesion properties and caused a 2-fold decrease in S. enterica serovar Typhimurium UK1-lux invasion of HT-29 epithelial cells compared with bacteria grown in minimal medium alone. This could be related to changes in the hydrophobicity and fluidity of the membrane. Our study shows that technical properties underlying probiotic survivability can be affected by nutrient composition of the growth medium.

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Section: Discussionsupporting

confidence: 92%

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Müller

Ross

Sybesma

et al. 2011

Appl Environ Microbiol

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“…Before each measurement, bacterial cells were energized in 50 mM MES [2-(N-morpholino)ethanesulfonic acid]-KOH buffer (pH 7.2) containing 10 mM glucose to prevent a decrease in membrane fluidity caused by bacterial death independent of the stress nature (45). Membrane fluidity was determined continuously by measuring fluorescence anisotropy using hydrophobic 1,6-diphenyl-1,3,5-hexatriene (DPH) (Molecular Probes, Oregon) as a probe, as previously described in reference 13.…”

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confidence: 99%

Cyclopropanation of Membrane Unsaturated Fatty Acids Is Not Essential to the Acid Stress Response of Lactococcus lactis subsp. cremoris

Grandvalet

Tourdot-Maréchal

2011

Appl Environ Microbiol

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“…For instance, there are higher proportions of short-chain and/or branched-chain fatty acids and unsaturated fatty acids in cold-tolerant bacteria. A variety of other environmental factors, such as low water activity, low pH, and the presence of chemicals and antimicrobials, influence membrane fluidity (7,22,39). Thus, the ability of bacteria to change their membrane fluidities determines to some extent how well a bacterium tolerates certain environmental stresses.…”

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confidence: 99%

Temperature- and Surfactant-Induced Membrane Modifications That Alter Listeria monocytogenes Nisin Sensitivity by Different Mechanisms

Chikindas

Ludescher

et al. 2002

Appl Environ Microbiol

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Nisin interacts with target membranes in four sequential steps: binding, insertion, aggregation, and pore formation. Alterations in membrane composition might influence any of these steps. We hypothesized that cold temperatures (10°C) and surfactant (0.1% Tween 20) in the growth medium would influence Listeria monocytogenes membrane lipid composition, membrane fluidity, and, as a result, sensitivity to nisin. Compared to the membranes of cells grown at 30°C, those of L. monocytogenes grown at 10°C had increased amounts of shorter, branched-chain fatty acids, increased fluidity (as measured by fluorescence anisotropy), and increased nisin sensitivity. When 0.1% Tween 20 was included in the medium and the cells were cultured at 30°C, there were complex changes in lipid composition. They did not influence membrane fluidity but nonetheless increased nisin sensitivity. Further investigation found that these cells had an increased ability to bind radioactively labeled nisin. This suggests that the modification of the surfactant-adapted cell membrane increased nisin sensitivity at the binding step and demonstrates that each of the four steps can contribute to nisin sensitivity.Listeria monocytogenes is a gram-positive, non-spore-forming food-borne pathogen that can grow at refrigeration temperatures (31, 41). L. monocytogenes has a sophisticated strategy to overcome environmental stresses, such as cold (23, 38), heat (35), an acidic environment (27), and osmotic shock (4). L. monocytogenes is of particular interest with respect to food safety due to its psychrotrophic nature (41), which may result in the enrichment of L. monocytogenes in foods at refrigeration temperature (15). This organism can pose a serious health threat to high-risk populations, such as immunocompromised individuals, newborns, and pregnant women. (42).Nisin is used as an antimicrobial agent against L. monocytogenes. The bactericidal activity of nisin is due to pore formation in the bacterial membrane (12), which occurs through a four-step process of binding, insertion, aggregation, and pore formation. The cell's sensitivity to nisin is influenced by the membrane's lipid composition (24), which might act on any of the four steps. Nisin-resistant L. monocytogenes has a lowered cellular phospholipid content and an altered membrane fatty acid composition. (11,24,25).The cytoplasmic membrane is the primary barrier between the cell and its environment. Environmental factors, especially temperature, influence membrane fluidity by modifying the membrane's lipid composition (19,23). The process of maintaining fluidity, called homeoviscous adaptation (34,29,45), is critical for cell growth at nonoptimal temperatures (5, 20). The concept of membrane fluidity covers the thermal mobility of membrane proteins as well as the microviscosity of the membrane lipid bilayer (33). However, the primary way that bacteria maintain constant membrane fluidity at different growth temperatures is by adjusting their fatty acid compositions (1,5,29). For instance, there are hig...

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Membrane fluidity of stressed cells of Oenococcus oeni

Cited by 64 publications

References 10 publications

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Cyclopropanation of Membrane Unsaturated Fatty Acids Is Not Essential to the Acid Stress Response of Lactococcus lactis subsp. cremoris

Temperature- and Surfactant-Induced Membrane Modifications That Alter Listeria monocytogenes Nisin Sensitivity by Different Mechanisms

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