Membrane Fusion

Jahn, Reinhard; Lang, Thorsten; Südhof, Thomas C.

doi:10.1016/s0092-8674(03)00112-0

Cited by 1,356 publications

(1,164 citation statements)

References 144 publications

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“…SNAP25 belongs to a family essential for synaptic and secretory vesicle exocytosis 32. To the best of our knowledge, there is no report of SNAP25 expression in malignancies.…”

Section: Discussionmentioning

confidence: 99%

Expressions of SH3BP5, LMO3, and SNAP25 in diffuse large B‐cell lymphoma cells and their association with clinical features

et al. 2016

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Diffuse large B‐cell lymphoma (DLBCL) is clinicopathologically and genetically heterogeneous with variable clinical outcomes. We previously identified signature genes overexpressed in CD5‐positive (CD5+) DLBCL, which is a poor prognostic subgroup of DLBCL. To elucidate the clinical significance of the protein expression of the signature genes overexpressed in CD5+ DLBCL with regard to all DLBCL, not otherwise specified (NOS), 10 genes (SH3BP5,LMO3,SNAP25,SYT5,SV2C,CABP1,FGF1,FGFR2,NEUROD1, and SYN2) were selected and examined immunohistochemically with samples from 28 patients with DLBCL, NOS. Only three protein expressions, SH3BP5, LMO3, and SNAP25, were detected in DLBCL cells and then analyzed further with samples from 187 patients with DLBCL, NOS. The SH3BP5, LMO3, and SNAP25 proteins were expressed in 60% (103/173), 34% (59/175), and 46% (77/168) of DLBCL patients, respectively. These protein expressions were associated with CD5 expression, and only SH3BP5 was frequently expressed in activated B‐cell‐like DLBCL (P = 0.046). Compared to the SH3BP5‐negative group, the SH3BP5+ group was correlated with elderly onset (>60 years, P = 0.0096) and advanced‐stage disease (stage III/IV, P = 0.037). The LMO3+ group showed a worse performance status (>1, P = 0.0004). The SH3BP5+ group and the LMO3+ group had significantly worse overall survival than the negative groups (P = 0.030, 0.034; respectively) for the entire group. In a subgroup analysis of patients treated with rituximab‐containing chemotherapy, there was no significant difference between groups. To the best of our knowledge, this is the first report showing the protein expressions of SH3BP5, LMO3, and SNAP25 in DLBCL cells and their clinical significance in patients with DLBCL. The SH3BP5 and LMO3 protein expressions are associated with the baseline clinical characteristics of DLBCL.

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“…SNAP25 belongs to a family essential for synaptic and secretory vesicle exocytosis 32. To the best of our knowledge, there is no report of SNAP25 expression in malignancies.…”

Section: Discussionmentioning

confidence: 99%

Expressions of SH3BP5, LMO3, and SNAP25 in diffuse large B‐cell lymphoma cells and their association with clinical features

et al. 2016

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“…SNARE proteins are important for docking and fusion of the transport vesicles to target membranes (see review in Ref. 38). Cleavage of vesicle-associated membrane protein caused defective synaptic vesicle biogenesis in PC12 cells (39).…”

Section: Discussionmentioning

confidence: 99%

Reticulon 3 Binds the 2C Protein of Enterovirus 71 and Is Required for Viral Replication

Tang¹,

Yang²,

Wu³

et al. 2007

Journal of Biological Chemistry

141

124

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Enterovirus 71 is an enterovirus of the family Picornaviridae. The 2C protein of poliovirus, a relative of enterovirus 71, is essential for viral replication. The poliovirus 2C protein is associated with host membrane vesicles, which form viral replication complexes where viral RNA synthesis takes place. We have now identified a host-encoded 2C binding protein called reticulon 3, which we found to be associated with the replication complex through direct interaction with the enterovirus 71-encoded 2C protein. We observed that the N terminus of the 2C protein, which has both RNA-and membrane-binding activity, interacted with reticulon 3. This region of interaction was mapped to its reticulon homology domain, whereas that of 2C was encoded by the 25th amino acid, isoleucine. Reticulon 3 could also interact with the 2C proteins encoded by other enteroviruses, such as poliovirus and coxsackievirus A16, implying that it is a common factor for such viral replication. Reduced production of reticulon 3 by RNA interference markedly reduced the synthesis of enterovirus 71-encoded viral proteins and replicative doublestranded RNA, reducing plaque formation and apoptosis. Furthermore, reintroduction of nondegradable reticulon 3 into these knockdown cells rescued enterovirus 71 infectivity, and viral protein and double-stranded RNA synthesis. Thus, reticulon 3 is an important component of enterovirus 71 replication, through its potential role in modulation of the sequential interactions between enterovirus 71 viral RNA and the replication complex.

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“…Membrane fusion plays an important role in a wide range of physiological processes as cell growth, membrane repair, cytokinesis, intracellular transport or synaptic transmission 1 . The fusion of membranes is typically driven by proteins (SNAREs, Hemagglutinin) [1][2][3][4][5][6] or by divalent cations in the case of synthetic vesicles [7][8][9] . But despite of a large number of studies during the last 30 years, many aspects of membranes fusion stayed controversial like the pathways to fusion in the case of protein-free lipid bilayers [1][2][3][4][5][6][7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

“…But despite of a large number of studies during the last 30 years, many aspects of membranes fusion stayed controversial like the pathways to fusion in the case of protein-free lipid bilayers [1][2][3][4][5][6][7][8][9][10] . Several studies are suggesting that the pathway to fusion begins with an initial hemifused region of minimum size (stalk), where the outer leaflets of the opposing membranes are fused while the inner leaflets engage in a new bilayer region called the hemifusion diaphragm (HD) [1][2][3][4][5][6][7][8][9][10][11] . Subsequently, the HD region is expanding until an equilibrium hemifused state is reached, unless the (HD) membrane tension is sufficient to trigger the membrane fusion [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Fast membrane hemifusion via dewetting between lipid bilayers

2014

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The behavior of lipid bilayer is important to understand the functionality of cells like the trafficking of ions between cells. Standard procedures to explore the properties of lipid bilayer and hemifused states typically use either supported membranes or vesicles. Both techniques have several shortcoming in terms of bio relevance or accessibility for measurements. In this article the formation of individual free standing hemifused states between model cell membranes is studied using an optimized microfluidic scheme wh ich allows for simultaneous optical and electrophysiological measurements. In a first step, two model membranes are formed at a desired location within a microfluidic device using a variation of the droplet interface bilayer (DiB) technique. In a second st ep, the two model membranes are brought into contact forming a single hemifused state. For all tested lipids, the hemifused state between free standing membranes form within hundreds of milliseconds, i.e. several orders of magnitude faster than reported in literature. The formation of a hemifused state is observed as a two stage process, whereas the second stage can be explained as a dewetting process in no-slip boundary condition. The formed hemifusion states are long living and a single fusion event can be observed when triggered by an applied electric field as demonstrated for monoolein.

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Membrane Fusion

Cited by 1,356 publications

References 144 publications

Expressions of SH3BP5, LMO3, and SNAP25 in diffuse large B‐cell lymphoma cells and their association with clinical features

Expressions of SH3BP5, LMO3, and SNAP25 in diffuse large B‐cell lymphoma cells and their association with clinical features

Reticulon 3 Binds the 2C Protein of Enterovirus 71 and Is Required for Viral Replication

Fast membrane hemifusion via dewetting between lipid bilayers

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