Membrane Fusion and Deformation of Red Blood Cells by Electric Fields

Scheurich, Peter; Zimmermann, U.; Mischel, Maja; Lamprecht, I.

doi:10.1515/znc-1980-11-1236

Cited by 39 publications

(11 citation statements)

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“…3b). Much higher field strengths result in stretching and deformation of the cells, budding and vesicle formation, particularly if the field strength of the a-c field exceeds the breakdown voltage of the membrane [20,58]. At the same time, streaming of the cytoplasm and the organelles is observed (unpublished results) very similar to the phenomenon of cyclosis seen in plant cells and slime molds [3,29,66,67].…”

Section: Membrane Contactmentioning

confidence: 72%

Electric field-induced cell-to-cell fusion

1982

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Cell-to-cell fusion has great potential in membrane research, membrane reconstitution and genetic mapping [25,34,49]. Furthermore, both somatic hybridization and genetic engineering offer a way of modifying plant cells and, in turn, of improving crops [49,53]. If lymphocyte cells are fused with a permanent cell line so-called hybridoma cells are produced [32] which are capable of producing monoclonal antibodies of predetermined antigenic specificity. These hybridoma cells (antibody-producing hybrids) can be envisaged as important tools in future clinical diagnosis and therapy as well as in the purification and enrichment of compounds of cell-biological and medical interest [18,65, 72]. However, despite many promising results and considerable efforts, fusion still seems to be something of an art rather than a precise science.Phenomenologically, cell-to-cell fusion is achieved presently in vitro by chemicals or inactivated virus [2,30,31,33,34,48,64]. Fusion can usually only be achieved by using membrane-disrupting agents and procedures and/or unphysiological conditions (e.g., high Ca/+ concentrations, high or low pH values, hypotonic conditions, etc.)The field of chemically and virus-induced fusion has been excellently reviewed by several authors over the last years [17,34,45,47,48]. In view of the bewildering array of data on chemically and virusinduced fusion we are obliged to conclude that we are still far from solving the molecular mechanism underlying the fusion process.Any progress in this field and in the development of novel fusion techniques will have to be evaluated by comparison with the inherent limitations of the current fusion procedures, which are listed below:

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Section: Membrane Contactmentioning

confidence: 72%

Electric field-induced cell-to-cell fusion

1982

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“…Further on, high voltage fields are an useful tool for cell fusion experiments which have been established with protoplasts of plant cells as well as blood cells in order to produce hybrids of different cell types [17,18].…”

Section: Introductionmentioning

confidence: 99%

Electric field effects on bacteria and yeast cells

Hülsheger

Potel

Niemann

1983

Radiat Environ Biophys

306

142

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Comparative studies were carried out describing the lethal effects of electric pulses on GRAM-negative bacteria, GRAM-positive bacteria, and yeast cells. Microorganisms are killed by the pulse treatment without visible morphological destruction. The observed survival rates are figured as functions of the field strength E and the treatment time t (pulse number X time constant) revealing three explicit parameters as sufficient to explain the kinetics of the results. These parameters are determined by the species of microorganism used and moreover depend on the physiological properties of the microbial population. GRAM-positive bacteria and yeasts were found to be less sensitive to electric pulse treatment than GRAM-negative bacteria, when low pulse numbers are applied. Treatment with high pulse numbers reveals survival rates below 1% for all microorganisms examined. Cells from the logarithmic growth phase are killed in markedly higher percentage than cells harvested from the stationary growth phase. The obtained results as well as further studies confirm the hypothesis of an electric induced selective damage of inner cell membranes.

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“…Somatic cell hybrid cell lines (or more simply hybrid cell lines) are cell lines that arise through intentional or nonintentional fusion of somatic cells having different origins [2]. Intra-species and inter-species (cross-species) cell fusions have been described since the 1950s [3] and can occur either spontaneously or can be mediated by human oncogenic viruses (such as Sendai virus, Epstein-Barr virus, human papilloma viruses, hepatitis B and C viruses, human T-cell lymphotropic virus type 1 (HTLV-1), herpesviruses-8/Kaposi sarcoma herpesvirus (HHV-8/KSHV)) [4,5], polyethylene glycol [6], or electrical pulses (electrofusion) [7][8][9][10][11][12], resulting in viable syncytial cells (giant cells or polykaryotes) with hybrid genotypes, namely heterokaryons. Mouse-human heterohybridoma technology has advanced significantly with the use of electrofusion technology [13].…”

Section: Introductionmentioning

confidence: 99%

Authenticating Hybrid Cell Lines

Nims¹,

Capes‐Davis²,

Korch³

et al. 2019

Cell Culture

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Hybrid (both intra-species and inter-species) cell lines arise through intentional or nonintentional fusion of somatic cells having different origins. Hybrid cell lines can pose a problem for authentication testing to confirm cell line identity, since the results obtained may not conform to the results expected for the two parental cell types. Thus, depending on the identity testing methodology, a hybrid cell may display characteristics of one of the parental cell type or of both. In some instances, the hybrid cell line may display characteristics that are different from those displayed by either parental cell type; these differences may not necessarily indicate cellular cross-contamination. Testing should be performed as soon as possible after an intended fusion has occurred, so that a baseline reference profile is available for later comparison. In this article, we describe the various approaches that have been used for identifying hybrid cell lines and the results that might be expected when using various technologies for this purpose.

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Membrane Fusion and Deformation of Red Blood Cells by Electric Fields

Cited by 39 publications

References 0 publications

Electric field-induced cell-to-cell fusion

Electric field-induced cell-to-cell fusion

Electric field effects on bacteria and yeast cells

Authenticating Hybrid Cell Lines

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