Membrane fusion of phospholipid bilayers under high pressure: Spherical and irreversible growth of giant vesicles

Goto, Masaki; Kazama, Akira; Fukuhara, Kensuke; Sato, Honami; Tamai, Naoto; Ito, Hiroo; Matsuki, Hitoshi

doi:10.1016/j.bpc.2021.106639

Cited by 9 publications

(7 citation statements)

References 63 publications

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“…Accordingly, the liposomes should collect at the bottom of the bubble (in the narrow space between the bubble surface and the plasmonic substrate) and be subjected to a high pressure. It was recently reported that high pressure frequently induced MF of vesicles . We consider the high pressure near the center of the bottom of the bubble to be the origin of the MF.…”

Section: Discussionmentioning

confidence: 86%

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Generation of Ultralong Liposome Tubes by Membrane Fusion beneath a Laser-Induced Microbubble on Gold Surfaces

et al. 2022

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Membrane fusion (MF) is one of the most important and ubiquitous processes in living organisms. In this study, we developed a novel method for MF of liposomes. Our method is based on laser-induced bubble generation on gold surfaces (a plasmonic nanostructure or a flat film). It is a simple and quick process that takes about 1 min. Upon bubble generation, liposomes not only collect and become trapped but also fuse to form long tubes beneath the bubble. Moreover, during laser irradiation, these long tubes remain stable and move with a waving motion while continuing to grow, resulting in the creation of ultralong tubes with lengths of about 50 μm. It should be noted that the morphology of these ultralong tubes is analogous to that of a sea anemone. The behavior of the tubes was also monitored by fluorescence microscopy. The generation of these ultralong tubes is discussed on the basis of Marangoni convection and thermophoresis.

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Section: Discussionmentioning

confidence: 86%

“…It was recently reported that high pressure frequently induced MF of vesicles. 26 We consider the high pressure near the center of the bottom of the bubble to be the origin of the MF. With such MF, giant liposomes should be generated.…”

Section: Discussionmentioning

confidence: 99%

Generation of Ultralong Liposome Tubes by Membrane Fusion beneath a Laser-Induced Microbubble on Gold Surfaces

et al. 2022

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“…Therefore, to increase fusion cell viability, an important factor to consider would be reduction in the duration of cell contact with PEG. Since previous research indicated that high pressure causes molecular distance to shrink [ 27 ], we hypothesized whether application of instantaneous pressure could replace the shaking process during cell fusion (Fig. 1 ).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of polyethylene glycol‐cell fusion efficiency by novel application of transient pressure using a jet injector

et al. 2023

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Cell–cell fusion involves the fusion of somatic cells into a single hybrid cell. It is not only a physiological process but also an important cell engineering technology which can be applied to various fields, such as regenerative medicine, antibody engineering, genetic engineering, and cancer therapy. There are three major methods of cell fusion: electrical cell fusion, polyethylene glycol (PEG) cell fusion, and virus‐mediated cell fusion. Although PEG cell fusion is the most economical approach and does not require expensive instrumentation, it has a poor fusion rate and induces a high rate of cell cytotoxicity. To improve the fusion rate of the PEG method, we combined it with the pyro‐drive jet injector (PJI). PJI provides instant pressure instead of cell agitation to increase the probability of cell‐to‐cell contact and shorten the distance between cells in the process of cell fusion. Here, we report that this improved fusion method not only decreased cell cytotoxicity during the fusion process, but also increased fusion rate compared with the conventional PEG method. Furthermore, we tested the functionality of cells fused using the PJI‐PEG method and found them to be comparable to those fused using the conventional PEG method in terms of their application for dendritic cell (DC)‐tumor cell fusion vaccine production; in addition, the PJI‐PEG method demonstrated excellent performance in hybridoma cell preparation. Taken together, our data indicate that this method improves cell fusion efficiency as compared to the PEG method and thus has the potential for use in various applications that require cell fusion technology.

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“…Although most of the direct isolation of membrane protein studies used bacteria, the use of mammalian and other cell types and other organisms such as viruses, insects, and fungi will become common in the near future. It would be exciting to explore the use of the nanodiscs technology for other membrane-related studies as well [ 67 , 219 , 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 ].…”

Section: Conclusion and Future Scopementioning

confidence: 99%

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

Krishnarjuna

Ramamoorthy

2022

Biomolecules

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Atomic-resolution structural studies of membrane-associated proteins and peptides in a membrane environment are important to fully understand their biological function and the roles played by them in the pathology of many diseases. However, the complexity of the cell membrane has severely limited the application of commonly used biophysical and biochemical techniques. Recent advancements in NMR spectroscopy and cryoEM approaches and the development of novel membrane mimetics have overcome some of the major challenges in this area. For example, the development of a variety of lipid-nanodiscs has enabled stable reconstitution and structural and functional studies of membrane proteins. In particular, the ability of synthetic amphipathic polymers to isolate membrane proteins directly from the cell membrane, along with the associated membrane components such as lipids, without the use of a detergent, has opened new avenues to study the structure and function of membrane proteins using a variety of biophysical and biological approaches. This review article is focused on covering the various polymers and approaches developed and their applications for the functional reconstitution and structural investigation of membrane proteins. The unique advantages and limitations of the use of synthetic polymers are also discussed.

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Membrane fusion of phospholipid bilayers under high pressure: Spherical and irreversible growth of giant vesicles

Cited by 9 publications

References 63 publications

Generation of Ultralong Liposome Tubes by Membrane Fusion beneath a Laser-Induced Microbubble on Gold Surfaces

Generation of Ultralong Liposome Tubes by Membrane Fusion beneath a Laser-Induced Microbubble on Gold Surfaces

Enhancement of polyethylene glycol‐cell fusion efficiency by novel application of transient pressure using a jet injector

Detergent-Free Isolation of Membrane Proteins and Strategies to Study Them in a Near-Native Membrane Environment

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