Membrane integrity evaluation in rabbit spermatozoa

Ducci, Michele; Gazzano, Angelo; Villani, Claudio; Cela, Vito; Artini, Paolo Giovanni; Martelli, Franco; Genazzani, Andrea Riccardo

doi:10.1016/s0301-2115(01)00578-4

Cited by 43 publications

(30 citation statements)

References 8 publications

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“…A modified hypo-osmotic swelling test procedure, combined with supravital staining technique described by Ducci et al (2002), was used to evaluate, simultaneously, functional integrity of both head and tail membranes of sperm cells. Briefly, semen samples diluted 1:10 in 60 mOsm fructose solution including 1% (w/v) Eosin Y, were incubated in a water bath at 36°C for 15 min.…”

Section: Assessment Of Membrane Integrity In Sperm Cellsmentioning

confidence: 99%

Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

2010

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Section: Assessment Of Membrane Integrity In Sperm Cellsmentioning

confidence: 99%

Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

2010

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“…The osmotic resistance was assessed by modified cumulative analysis of hypoosmotic eosin staining test (HE-test), using the eosin exclusion test and the Hypoosmotic Swelling Test (HOST) (Ducci et al, 2002, Fukui et al, 2004, Mansour, 2009. Semen samples were diluted 1:10 (v/v) in 100 mOsm fructose solution including 1% (w/v) eosin-Y, and were incubated in a water bath at 35ºC for 30 minutes.…”

Section: Evaluation Of Epidydimal Sperm Parametersmentioning

confidence: 99%

The Effect of Lyophilized Pomegranate Extract on Epididymal Sperm Quality, Oxidative Stress and Spermatogenic Cell Density in Rabbits

Avdatek

Yeni

Başer

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. The aim of this study was to investigate the effect of lyophilized pomegranate extract on spermatological features, pathology of testes and total antioxidant/oxidant status in rabbits. Adult male rabbits were divided into four groups containing six rabbits each. For 8 weeks, Group I received standard diet and 1 ml % 0.5 carboxymethyl cellulose (CMC), Group II received 25 mg/kg/day pomegranate extract + 1 ml % 0.5 carboxymethyl cellulose (CMC), Group III received 50 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC and Group IV received 100 mg/kg/day pomegranate extract + 1 ml % 0.5 CMC. Rabbits were sacrificed by using xylazine 5 mg/kg+ketamine 35 mg/kg anaesthesia and were euthanized by 150 mg / kg intraperitoneal thiopental sodium at the end of the eighth week. Spermatozoon motility, abnormal sperm rate, sperm membrane integrity, total antioxidant/oxidant level and spermatogenic cell density were investigated. All analyses were done only once at the end of study period. Data were compared by analysis of variance (ANOVA) and the degree of significance was set at (p<0.05). Sperm motility and membrane integrity increased significantly (p<0.05) in groups II, III and IV; abnormal sperm rate decreased significantly (p<0.05) in groups III, IV; total oxidant status decreased significantly (p<0.05) in group IV in comparison to the control group. Seminiferous tubule diameter increased significantly (p<0.05) in all groups compared to the control group. Germinal cell layer thickness significantly increased (p<0.05) in group IV compared to the control group. Results of this study suggest that 50 mg/kg/day + 1 ml % 0.5 CMC and 100 mg/kg/day + 1 ml % 0.5 CMC improve sperm parameters in rabbits.

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“…Combined with Eosin Y staining, HOS-T allows for evaluating the status of the sperm membrane in the tail and head. Four types of sperm were thus identified (Ducci et al, 2002).…”

Section: Membrane Integrity Assay (Hos-t)mentioning

confidence: 99%

Effects ofHeliopsis longipesethanolic extract on mouse spermatozoain vitro

Martinez-Loredo

Izquierdo-Vega

Cariño-Cortés

et al. 2015

Pharmaceutical Biology

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Context: Heliopsis longipes (A. Gray) Blake (Asteraceae), a plant native to Mexico, is used in traditional medicine as analgesic and microbicide. The main component in the H. longipes ethanolic extract (HLEE) is affinin, as determined by HPLC/UV-visible and NMR measurement. To date, there is no documented evidence on the spermicidal activity of this extract. Objective: The objective of this study was to assess in vitro the effectiveness of HLEE as spermicide. Materials and methods:The spermicidal activity of HLEE was evaluated by the Sander-Cramer assay. Spermatozoa were incubated for 20 s with HLEE in concentrations ranging from 75 to 2000 mg/mL to determine the minimum effective concentration (MEC) value. The 50% effective concentration (EC 50 ) of HLEE was estimated by assaying serial dilutions from the MEC. Additionally, sperms were incubated with 125, 250, or 500 mg/mL of HLEE to evaluate the viability and the integrity of sperm membrane. Lipid peroxidation was assessed by the thiobarbituric acid reactive substances assay. Results: HLEE caused an inhibition of 100% in spermatozoa motility at a MEC value of 2000 mg/mL; the EC 50 value was 125 mg/mL. Additionally, exposure to HLEE at 125, 250, or 500 mg/mL for 30 min decreased sperm viability to 27%, 8%, and 2% of the control value, respectively, and significantly increased the percentage of sperms with structurally disorganized membrane. HLEE also increased significantly the level of lipid peroxidation in sperms with respect to controls. Discussion and conclusion: The results demonstrate the spermicidal activity of HLEE in vitro and suggest that this action is caused by oxidative damage and alterations in the spermatozoal membrane.

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Membrane integrity evaluation in rabbit spermatozoa

Cited by 43 publications

References 8 publications

Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4ºC or freezing.

The Effect of Lyophilized Pomegranate Extract on Epididymal Sperm Quality, Oxidative Stress and Spermatogenic Cell Density in Rabbits

Effects ofHeliopsis longipesethanolic extract on mouse spermatozoain vitro

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