Membrane‐Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) WiredviaSpecific Os‐Redox Polymers

Abstract

A membrane‐free biofuel cell (BFC) is reported based on enzymes wired to graphite electrodes by means of Os‐complex modified redox polymers. For the anode cellobiose dehydrogenase (CDH) is used as a biocatalyst whereas for the cathode a laccase was applied. This laccase is a high‐potential laccase and hence able to reduce O2 to H2O at a formal potential higher than +500 mV versus Ag/AgCl. In order to establish efficient electrochemical contact between the enzymes and graphite electrodes electrodeposition polymers containing Os‐complex with specifically designed monomer compositions and formal potentials of the coordinatively bound Os‐complex were synthesised and used to wire the enzymes to the electrodes. The newly designed CDH/Os‐redox polymer anode was characterised at different pH values and optimised with respect to the nature of the polymer and the enzyme‐to‐polymer ratio. The resulting BFC was evaluated running on β‐lactose as a fuel and air/O2 as an oxidising agent. The power output, the maximum current density and the electromotor force (Eemf) were found to be affected by the pH value, resulting in a maximum power output of 1.9 μW cm–2 reached at pH 4.3, a maximum current density of about 13 μA cm–2 at pH 3.5, and the highest Eemf approaching 600 mV at pH 4.0.

A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

“…These DET-and MET-based devices, however, operated under acidic conditions because both bioelements used in the BFCs, viz. cellobiose dehydrogenase (CDH) from Dichomera saubinetii (DsCDH) [11] or from Trametes villosa (TvCDH) [12] and laccase (Lc) from Trametes hirsuta (ThLc) [11] or from Cerena unicolor (CuLc) [12], have their bioelectrocatalytic activity optima at acidic pH values [14,15].…”

Layer-by-Layer Assembly of Carbon Nanotubes Modified with Invertase/Glucose Dehydrogenase Cascade for Sucrose/O2Biofuel Cell

“…This generated 42 ± 15 μW/cm 2 in 100 mmol/L sucrose. Several other reports include trehalose, 33 cellobiose, 34 and starch 35 based fuel cells obtaining maximum current density of 0.1 mA/cm 2 , 1.9 μW/cm 3 and 8.15 μW/cm 3 , respectively. Handa et al…”

“…This generated 42 ± 15 μW/cm 2 in 100 mmol/L sucrose. Several other reports include trehalose, 33 cellobiose, 34 and starch 35 based fuel cells obtaining maximum current density of 0.1 mA/cm 2 , 1.9 μW/cm 3 and 8.15 μW/cm 3 , respectively. Handa et al 36 fabricated a carbon-felt based invertase, fructose dehydrogenase and glucose oxidase immobilized bioanode mediated by tetrathiafulvalene and bilirubin oxidase immobilized biocathode with ABTS as mediator for sucrose biofuel cell.…”

Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-complex containing redox polymer with entrapped Trametes hirsuta laccase

“…This concept was already applied to the design of a biofuel cell cathode modified with bilirubin oxidase from Myrothecium verrucaria using an Os-complex modified anodic polymer [21]. Additionally, a complete membrane-less biofuel cell using cellobiose dehydrogenase from Trametes villosa at the anode and a high-potential laccase from Cerrena unicolor at the cathode was demonstrated [22].…”