Membrane lipid peroxidation in lichens determined by the TBARS assay as a suitable biomarker for the prediction of elevated level of potentially toxic trace elements in soil

Osyczka, Piotr; Chowaniec, Karolina; Rola, Kaja

doi:10.1016/j.ecolind.2023.109910

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…The amount of nitric oxide (NO), lipid peroxidation (TBARS), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2) were determined in supernatants of homogenized rat paw tissue [ 51 ]. Briefly, the nitric oxide was determined as the reduction of the Griess reagent at a wavelength of 540 nm.…”

Section: Biochemical Analysismentioning

confidence: 99%

Synthesis, Computational Studies, Antioxidant and Anti-Inflammatory Bio-Evaluation of 2,5-Disubstituted-1,3,4-Oxadiazole Derivatives

et al. 2023

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The 1,3,4-oxadiazole derivatives Ox-6a-f have been synthesized by incorporating flurbiprofen moiety with the aim to explore the potential of target molecules to decrease the oxidative stress. The title compounds Ox-6a-f were prepared by simple reactions in which a flurbiprofen –COOH group was esterified with methanol in an acid-catalyzed medium, which was then reacted with hydrazine to afford the corresponding hydrazide. The acid hydrazide was then cyclized into 1,3,4-oxadiazole-2-thiol by reacting with CS2 in the presence of KOH. The title compounds Ox-6a-f were synthesized by the reaction of an –SH group with various alkyl/aryl chlorides, which involves an S-alkylation reaction. The structures of the synthesized Ox-6a-f derivatives were ascertained by spectroscopic data. The in silico molecular docking was performed against target proteins cyclooxygenase-2 COX-2 (PDBID 5KIR) and cyclooxygenase-1 COX-1 (PDBID 6Y3C) to determine the binding affinity of the synthesized compounds with these structures. It has been inferred that most of the synthesized compounds bind well with an active binding site of 5KIR compared to 6Y3C, and especially compound Ox-6f showed excellent binding affinity (7.70 kcal/mol) among all synthesized compounds Ox-6a-f. The molecular dynamic (MD) simulation has also been performed to check the stability of docking complexes of ligands with COX-2 by determining their root mean square deviation and root mean square fluctuation. Little fluctuation was observed in case of Ox-6f, which forms the most stable complex with COX-2. The comprehensive antioxidant potential of the synthesized compounds has been evaluated by determining their free radical scavenging activity, including DPPH, OH, nitric oxide (NO), and iron chelation assay. The derivative Ox-6f showed promising results with 80.23% radical scavenging potential at a dose of 100 µg/mL while ascorbic acid exhibited 87.72% inhibition at the same dose. The anti-inflammatory activity of the final products has also been performed, and inflammatory markers were assayed, such as a thiobarbituric acid-reducing substance, nitric oxide, interleukin-6 (IL-6), and COX-2. The derivatives Ox-6d and Ox-6f displayed higher anti-inflammatory activity, exhibiting 70.56% and 74.16% activity, respectively. The results were compared with standard ibuprofen, which showed 84.31% activity at the same dose, 200 µg/mL. The anti-inflammatory potential has been performed by following the carrageen-induced hind paw edema model, and results showed that derivative Ox-6f exhibited 79.83% reduction in edema volume compared to standard ibuprofen, which reduced 84.31% edema volume. As dry lab and wet lab results confirm each other, it has been deduced that derivative Ox-6f may serve as the lead structure to design potent compounds to address oxidative stress.

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Section: Biochemical Analysismentioning

confidence: 99%

Synthesis, Computational Studies, Antioxidant and Anti-Inflammatory Bio-Evaluation of 2,5-Disubstituted-1,3,4-Oxadiazole Derivatives

et al. 2023

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Chia seed protein as a promising source for plant-based foods: Functional properties, processing methods and potential food applications

Chen,

Luo

2024

Applied Food Research

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Geçmişten günümüze potansiyel hammadde kaynağı: Likenler

TOKSÖZ

2023

Frontiers in Life Sciences and Related Technologies

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Likenler içerdikleri 1000’den fazla metabolite sayesinde antioksidan, antimikrobiyal, antifungal, instektisidal, antikanser ve boyar madde potansiyelleri gibi biyolojik etkinliklerinden dolayı çeşitli sektörlerde potansiyel bir hammadde kaynağı olarak tercih edilmektedir. Yüzlerce yıldır etnofarmakolojik olarak bir çok hastalığın tedavisinde halk arasında kullanılmasının yanı sıra günümüzde hala likenlerin ilaç potansiyelleri araştırılmaya devam edilmektedir. Likenlerin aromatik ve besleyici özellikleri nedeniyle, gıda sektöründe baharat, ekmekçilik, pastacılık ve çay olarak kullanımları devam etmektedir. Likenlerin ekonomik anlamda en önemli kullanım alanlarından biri olan boyar madde içerikleri nedeniyle başta tekstil sektörü olmak üzere bir çok sektörde tercih edilmektedir. Ayrıca tarımsal alanda ise fitopatojenlere karşı insektisidal ve antifungal etkinliğe sahip oldukları bilinmektedir. Likenlerin ve içerdikleri metabolitlerin yalnızca bir kısmının etkinlikleri biliniyor olsada, tüm özellikleri hala tam olarak aydınlatılmamıştır. Bu bağlamda, liken ve etken maddelerinin biyoaktivitelerinin gelecekte açığa çıkartılmasıyla birlikte, birçok sektörde potansiyel hammadde olarak kullanılması öngörülmektedir.

show abstract

Membrane lipid peroxidation in lichens determined by the TBARS assay as a suitable biomarker for the prediction of elevated level of potentially toxic trace elements in soil

Cited by 3 publications

References 47 publications

Synthesis, Computational Studies, Antioxidant and Anti-Inflammatory Bio-Evaluation of 2,5-Disubstituted-1,3,4-Oxadiazole Derivatives

Synthesis, Computational Studies, Antioxidant and Anti-Inflammatory Bio-Evaluation of 2,5-Disubstituted-1,3,4-Oxadiazole Derivatives

Chia seed protein as a promising source for plant-based foods: Functional properties, processing methods and potential food applications

Geçmişten günümüze potansiyel hammadde kaynağı: Likenler

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