Membrane Lipids and Aging

Shmeeda, Hilary; Golden, Elisabeth B.; Barenholz, Y.

doi:10.1002/9783527616114.ch1

Cited by 6 publications

(10 citation statements)

References 388 publications

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“…in Barenholz and Thompson, 1980;Shmeeda et al, 1994a). How can one explain such co-localization, especially since in higher eukaryotes syntheses of glycerophospholipids and cholesterol occur mainly in the endoplasmic reticulum, whereas sphingomyelin and higher glycosphingolipids are synthesized in the Golgi apparatus?…”

Section: 1mentioning

confidence: 97%

“…in Yeagle, 1985Yeagle, , 1988Yeagle, , 1993a. Although these effects can be attributed to cholesterol's biophysical contribution to membrane properties, there are also indications that direct effects (not yet clear) on the proteins may also be involved (Shmeeda et al, 1994a;Yeagle, 1988Yeagle, , 1993aBarenholz and Cevc, 2000;Mitchell and Litman, 2001). …”

Section: Introducing Cholesterolmentioning

confidence: 93%

“…This rate is much faster than the desorption rate of almost all membrane-forming lipids (rev. in Yeagle, 1988;Shmeeda et al, 1994a). Sphingomyel~n is a "liposome-forming" lipid, which means that in aqueous phase it can form liposomes by itself, while cholesterol, having a much larger packing parameter (see 3.2.1 below), is not a liposome-forming lipid.…”

Section: 1mentioning

confidence: 98%

“…in Shmeeda et al, 1994a), during the development of atherosclerosis (rev. in Barenholz and Thompson, 1980;Barenholz, 1984), and in various types of Niemann-Pick disease (rev.…”

Section: 1mentioning

confidence: 99%

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Sphingomyelin and Cholesterol: From Membrane Biophysics and Rafts to Potential Medical Applications

Barenholz

2004

Subcellular Biochemistry

120

101

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The preferential sphingomyelin-cholesterol interaction which results from the structure and the molecular properties of these two lipids seems to be the physicochemical basis for the formation and maintenance of cholesterol/sphingolipid-enriched nano-and micro-domains (referred to as membrane "rafts") in the plane of plasma and other organelle (i.e., Golgi) membranes. This claim is supported by much experimental evidence and also by theoretical considerations. However, although there is a large volume of information about these rafts regarding their lipid and protein composition, their size, and their dynamics, there is still much to be clarified on these issues, as well as on how rafts are formed and maintained. It is well accepted now that the lipid phase of the rafts is the liquid ordered (LO) phase. However, other (non-raft) parts of the membrane may also be in the LO phase.There are indications that the raft LO phase domains are more tightly packed than the non-raft LO phase, possibly due to intermolecular hydrogen bonding involving sphingolipid and cholesterol. This also explains why the former are detergent-resistant membranes (DRM), while the non-raft LO phase domains are detergent-soluble (sensitive) membranes (DSM).

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Section: 1mentioning

confidence: 97%

Section: Introducing Cholesterolmentioning

confidence: 93%

Section: 1mentioning

confidence: 98%

“…in Shmeeda et al, 1994a), during the development of atherosclerosis (rev. in Barenholz and Thompson, 1980;Barenholz, 1984), and in various types of Niemann-Pick disease (rev.…”

Section: 1mentioning

confidence: 99%

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Sphingomyelin and Cholesterol: From Membrane Biophysics and Rafts to Potential Medical Applications

Barenholz

2004

Subcellular Biochemistry

120

101

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“…It is well established that the matrix of these membranes, the lipid bilayer, has two faces that are compositionally distinct (for review, see Refs. [1][2][3]. At the level of resolution of the wavelength of visible light (ϳ0.5 m), studies based on determination of the immobile fraction in a fluorescence recovery after photobleaching experiment indicate that biological membranes are laterally heterogeneous and have in-plane domains distributed in a homogeneous lipid continuum (4 -6).…”

mentioning

confidence: 99%

Lateral Organization of Pyrene-labeled Lipids in Bilayers as Determined from the Deviation from Equilibrium between Pyrene Monomers and Excimers

Barenholz

Cohen

Haas

et al. 1996

Journal of Biological Chemistry

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In lipid bilayers, pyrene and pyrene-labeled lipids form excimers in a concentration-dependent manner. The aromatic amine N,N-diethylaniline (DEA), which has a high membrane-to-medium partition coefficient, quenches the monomers only, and therefore it is expected that under conditions in which the monomers are in equilibrium with the excimers due to the mass law, the Stern-Volmer coefficient (K sv ) for monomers (M), defined as K M , should be identical to that of the excimer (E), defined as K E , and K E /K M ‫؍‬ 1.0. This is indeed the case for pyrene and pyrene valerate in egg phosphatidylcholine small unilamellar vesicles. However, for pyrene decanoate and pyrene dodecanoate in these vesicles, and for N-[12-(1-pyrenyl)dodecanoyl]-sphingosylphosphocholine in a matrix of either N-stearoyl sphingosylphosphocholine or 1-palmitoyl-2-oleoyl phosphatidylcholine, K E < K M . This can be explained either by the existence of (a) two subpopulations of excimers, one in fast equilibrium with the monomers and the other, related to ground-state protoaggregates of pyrene lipids; (b) two monomer subpopulations where part of M cannot be quenched by DEA; or (c) two monomer subpopulations, both quenched by DEA, but only one of which produces excimers. The good agreement between the photophysical processes determined by steady state and time-resolved measurements supports the third explanation for the bilayers containing pyrene phospholipids. It also suggests that the main factors determining the immiscibility of pyrene lipids in phospholipid bilayers are the temperature, the difference in the gel-to-liquid-crystalline phase transition temperature (⌬T m ) between the matrix and the pyrene lipid, and the structural differences between the matrix lipid and the pyrene-labeled lipid. These results indicate that the K E /K M ratio can serve as a very sensitive tool to quantify isothermal microscopic immiscibility in membranes. This novel approach has the following advantages: applicability to fluid phase immiscibility, requirement of a relatively low mol fraction of pyrene lipids, and conceivably, applicability to biological membranes.Biological membranes are complex multicomponent assemblies. It is well established that the matrix of these membranes, the lipid bilayer, has two faces that are compositionally distinct (for review, see Refs. 1-3). At the level of resolution of the wavelength of visible light (ϳ0.5 m), studies based on determination of the immobile fraction in a fluorescence recovery after photobleaching experiment indicate that biological membranes are laterally heterogeneous and have in-plane domains distributed in a homogeneous lipid continuum (4 -6). More precise fluorescence recovery after photobleaching studies were recently performed in lipid bilayers of defined composition in which solid and fluid phases coexist, and the phase diagrams are well characterized (Refs. 7 and 8 and references therein). However, not much information is available on lateral organization at the submicron range. The functional significan...

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Lipophilic compounds in biotechnology—interactions with cells and technological problems1Dedicated to Professor Dr Wolfgang Fritsche on the occasion of his 65th birthday.12Based on the symposium, `Interactions between cells and lipophilic substances' held at the 8th European Congress on Biotechnology (ECB8) in Budapest, Hungary, August 1997.2

Angelova

Schmauder

1999

Journal of Biotechnology

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Membrane Lipids and Aging

Cited by 6 publications

References 388 publications

Sphingomyelin and Cholesterol: From Membrane Biophysics and Rafts to Potential Medical Applications

Sphingomyelin and Cholesterol: From Membrane Biophysics and Rafts to Potential Medical Applications

Lateral Organization of Pyrene-labeled Lipids in Bilayers as Determined from the Deviation from Equilibrium between Pyrene Monomers and Excimers

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