Membrane markers of the immune cells in swine: an update

Abstract

-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response

“…This might be related to the fact that LPS targets monocytes and macrophages [59] expressing CD14 and that PMA/ionomycin have a much wider spectrum of target cells. However, it cannot be ruled out that the significant difference in the number of differentially expressed genes according to stimulation is due to variations in the dynamics of the response.…”

Helicobacter pylori Infection in a Pig Model Is Dominated by Th1 and Cytotoxic CD8+T Cell Responses

“…In study 2, we performed a systematic assessment of Tbet expression in CD8ab ϩ T cells. Of note, CD8␤ is the most specific marker for CTL in pigs, since the CD8␣␣ homodimer is also expressed by other cell subsets (42). These cells specifically expressed low levels of the CD8␤ coreceptor, which is characteristic of CTL that have been previously exposed to their cognate antigen (43).…”

Induction of Foot-and-Mouth Disease Virus-Specific Cytotoxic T Cell Killing by Vaccination

“…The CD6 ϩ cell population has been characterized as consisting of MHC-restricted CTLs and helper T cells, including CD4 ϩ /CD8 Ϫ , CD4 Ϫ /CD8 ϩ , and CD4 ϩ /CD8 ϩ populations (8,11,41,42,49). Thus, an additional advantage of positively selecting for CD6 ϩ cells is the elimination of non-MHC-restricted cytolytic cells such as NK cells and ␥␦ T cells, which do not express CD6 (44).…”