Membrane mechanics govern spatiotemporal heterogeneity of endocytic clathrin coat dynamics

Abstract

Cellular processes associated with spatiotemporal changes in membrane mechanics induce significant alterations in clathrin-mediated endocytosis dynamics. This phenomenon is also observed during morphological changes shaping embryo development.

Deep learning enables cross-modality super-resolution in fluorescence microscopy

“…Clathrin dynamics were monitored in lateral epidermis and amnioserosa tissues of Drosophila embryos using the UAS/GAL4 system as described in ref. 38 . Drosophila embryos were gently pressed against the coverslip to position the apical surface of the lateral epidermis and amnioserosa cells within the evanescence field of the TIRF system.…”

Endocytic Clathrin Coats Develop Curvature at Early Stages of Their Formation

“…Plasma membrane tension is one of the most important determinants of endocytic clathrin coat structure and dynamics 19,[31][32][33][34] . Our automated analysis on genome edited SUM-159 cells expressing AP2-EGFP 35 revealed that average size of clathrin-coated pits increase when plasma membrane tension is elevated by hypo-osmotic shock ( Fig.…”

Mechanical Regulation of Endocytosis: New Insights and Recent Advances

“…Membrane-cytoskeleton adhesion enables the cytoskeleton to exert forces onto the plasma membrane. Hence, in-plane and cytoskeletal components of membrane tension are interdependent [23] and together they play a major role in regulating endocytosis [18,22,24,25] . Cellular processes associated with the creation of tension gradients can lead to spatio-temporal heterogeneity of endocytosis [8,16] .…”

“…Robust techniques to mechanically manipulate cells which are compatible with high-resolution microscopy, like cell stretchers, microcontact printing, stiffness tunable hydrogels, and micropillar arrays also gained progress in the last decade [12][13][14][15] . Automated algorithms for detecting and tracking endocytic events coupled with these sophisticated imaging techniques have enabled visualization of endocytosis in living organisms [16][17][18] .…”