Membrane microdomains in immunity: Glycosphingolipid‐enriched domain‐mediated innate immune responses

Iwabuchi, Kazuhisa; Nakayama, Hitoshi; Masuda, Hiromi; Kina, Katsunari; Ogawa, Hideoki; Takamori, Kenji

doi:10.1002/biof.1017

Cited by 18 publications

(15 citation statements)

References 62 publications

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“…Supporting the association mapping, the region on scaffold 626_944 matches with a genomic region previously identified by a QTL analysis of resistance to P. ramosa and which explained 50% of the observed variation for this trait (scaffold 944 in Routtu and Ebert (2015) is part of the super scaffold 626_944). In addition, this region displays a striking abundance of four functional categories involved in immune response or non-self recognitionfor example, chitinases (Lee et al 2011;Nance et al 2012;Beckerman et al 2013), serine proteases (Jang et al 2008), fucosyltransferases (Ma et al 2006;Davies 2009;Kashiwazaki et al 2014), and lactosylceramides (Iwabuchi et al 2012). Fucosyltransferase was the only function among the four categories for which a significant enrichment was observed in our region of interest.…”

Section: Discussionmentioning

confidence: 99%

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

et al. 2017

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Because parasitism is thought to play a major role in shaping host genomes, it has been predicted that genomic regions associated with resistance to parasites should stand out in genome scans, revealing signals of selection above the genomic background. To test whether parasitism is indeed such a major factor in host evolution and to better understand host-parasite interaction at the molecular level, we studied genome-wide polymorphisms in 97 genotypes of the planktonic crustacean Daphnia magna originating from three localities across Europe. Daphnia magna is known to coevolve with the bacterial pathogen Pasteuria ramosa for which host genotypes (clonal lines) are either resistant or susceptible. Using association mapping, we identified two genomic regions involved in resistance to P. ramosa, one of which was already known from a previous QTL analysis. We then performed a naïve genome scan to test for signatures of positive selection and found that the two regions identified with the association mapping further stood out as outliers. Several other regions with evidence for selection were also found, but no link between these regions and phenotypic variation could be established. Our results are consistent with the hypothesis that parasitism is driving host genome evolution.

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Section: Discussionmentioning

confidence: 99%

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

et al. 2017

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“…The complexes appeared to be cholesterol-dependent, as the trapping was reduced upon cholesterol depletion. STED microscopy was also used to demonstrate that CD11b integrin and LacCer are associated with the same "nanodomain" in the membrane of living neutrophils and participate in LacCer-mediated phagocytosis of microorganisms [274]. The major limitation of this technique is the necessity of using fluorescent probes or analogues or fluorescence-tagged antibodies against the target molecule, with the usual caveats related to the use of probes [275].…”

Section: Direct Detection Of Lipid Rafts At the Cell Surfacementioning

confidence: 99%

Membrane Domains and the Lipid Raft Concept

Sonnino¹,

Prinetti

2012

CMC

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The bulk structure of biological membranes consists of a bilayer of amphipathic lipids. According to the fluid mosaic model proposed by Singer and Nicholson, the glycerophospholipid bilayer is a two-dimensional fluid construct that allows the lateral movement of membrane components. Different types of lateral interactions among membrane components can take place, giving rise to multiple levels of lateral order that lead to highly organized structures. Early observations suggested that some of the lipid components of biological membranes may play active roles in the creation of these levels of order. In the late 1980s, a diverse series of experimental findings collectively gave rise to the lipid raft hypothesis. Lipid rafts were originally defined as membrane domains, i.e., ordered structures created as a consequence of the lateral segregation of sphingolipids and differing from the surrounding membrane in their molecular composition and properties. This definition was subsequently modified to introduce the notion that lipid rafts correspond to membrane areas stabilized by the presence of cholesterol within a liquid-ordered phase. During the past two decades, the concept of lipid rafts has become extremely popular among cell biologists, and these structures have been suggested to be involved in a great variety of cellular functions and biological events. During the same period, however, some groups presented experimental evidence that appeared to contradict the basic tenets that underlie the lipid raft concept. The concept is currently being redefined , with greater consistency regarding the true nature and role of lipid rafts. In this article we will review the concepts, criticisms, and the novel confirmatory findings relating to the lipid raft hypothesis.

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“…; Iwabuchi et al . ) and fluorescence correlation spectroscopy (Sezgin et al . )] have demonstrated the clustering and confinement of different glycosphingolipids at the plasma membrane level.…”

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confidence: 99%

Sphingolipids and neuronal degeneration in lysosomal storage disorders

Grassi

Chiricozzi

Mauri

et al. 2018

Journal of Neurochemistry

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Ceramide, sphingomyelin, and glycosphingolipids (both neutral and acidic) are characterized by the presence in the lipid moiety of an aliphatic base known as sphingosine. Altogether, they are called sphingolipids and are particularly abundant in neuronal plasma membranes, where, via interactions with the other membrane lipids and membrane proteins, they play a specific role in modulating the cell signaling processes. The metabolic pathways determining the plasma membrane sphingolipid composition are thus the key point for functional changes of the cell properties. Unnatural changes of the neuronal properties are observed in sphingolipidoses, lysosomal storage diseases occurring when a lysosomal sphingolipid hydrolase is not working, leading to the accumulation of the substrate and to its distribution to all the cell membranes interacting with lysosomes. Moreover, secondary accumulation of sphingolipids is a common trait of other lysosomal storage diseases. Keywords: gangliosides, lysosome, membrane fusion, sphingolipidoses, sphingolipids, sphingomyelin.This article is part of the Special Issue "Lysosomal Storage Disorders".Sphingolipids are amphiphilic membrane lipids characterized by the presence of sphingosine, an amino-and-hydroxy alkylic long chain. After more than a century of studies, we now know their structure, their physico-chemical properties, their distribution and content in cells and tissues of many animal species, including humans . The final cellular site for sphingolipids is the plasma membrane, however, their complex metabolic pathway requires many intracellular processes, so that a not negligible portion of them is also found intracellularly (Sandhoff and Kolter 2003;Schulze et al. 2009;Kolter and Sandhoff 2010;Hannun and Obeid 2018). As amphiphilic compounds they contain a hydrophilic head group, for example a saccharide, an oligosaccharide, a phosphocholine, protruding into the cell aqueous environment, and a hydrophobic chain called ceramide, in which the sphingosine is linked with a fatty acid via an amidic bond, inserted into the outer layer of plasma membrane.Sphingolipids are typically asymmetric components of the plasma membranes enriched in the outer leaflet (van Meer and Hoetzl 2010;van Meer 2011;Fujimoto and Parmryd 2016), however, it cannot be excluded that a minor pool of them could be transiently inserted into the inner layer with the hydrophilic group facing the cytosol. The hydrophilic group, thanks to the presence of carbohydrates and/or ionic charges, attracts water and ions, forms hydrogen bonds and easily interacts with the group of membrane components protruding from the membrane itself. At the water-lipid interface, the sphingolipid ceramide amide linkage accepts hydrogen bonds on the carbonyl group and donates hydrogen bonds via the N-H group, allowing the formation of a double linkage, which works as a padlock within molecules (Fig. 1). The lipid moiety inserted into the lipid layer interacts with the lipid portion of the cholesterol in the membrane and with dip...

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Membrane microdomains in immunity: Glycosphingolipid‐enriched domain‐mediated innate immune responses

Cited by 18 publications

References 62 publications

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

Membrane Domains and the Lipid Raft Concept

Sphingolipids and neuronal degeneration in lysosomal storage disorders

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