Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5‐hydroxytryptamine.

Berridge, Michael J.; Lindley, Barry D.; Prince, William

doi:10.1113/jphysiol.1975.sp010812

Cited by 96 publications

(70 citation statements)

References 9 publications

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“…Berridge et al (28) cnclfde" that increased intracellular calcium increased chloride permeability in the blowfly salivary gland. Chloride action potentials in the giant algal cell, Nitella, seem to be controlled by calcium (29).…”

Section: Resultsmentioning

confidence: 99%

Electrical currents through full-grown and maturing Xenopus oocytes.

Robinson¹

1979

Proc. Natl. Acad. Sci. U.S.A.

108

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An extracellular vibrating electrode was used to map the current pattern around Xenopus laevis oocytes. Current was found to enter the animal hemisphere and leave the vegetal hemisphere; in fully grown oocytes from which the follicle cells had been removed, the maximal current density was about 1 uA/cm2. This current decreased to nearly zero in response to progesterone and several other maturation-producing agents. In the case of progesterone, the decline began within a few minutes of the addition of the hormone and proceeded with a half-time of about 20 min.An analysis of the effects on the current of the removal or addition of various ions and drugs led to the inference that the major current-carrying ion was chloride and that the chloride permeability was controlled by calcium. An ubiquitous feature of nonmammalian animal eggs is the presence of an animal-vegetal axis. The nucleus of the egg is generally displaced toward the animal pole and this pole is the site of polar body formation. In many amphibian eggs, the axis is readily distinguished by the pigment accumulation at the animal pole and the yolk mass at the vegetal pole. Superimposed on this visible polarity is a developmental polarity: the animal pole gives rise to the ectodermal parts of the embryo and the vegetal pole, to the endodermal. (For a recent review, see refs. 1 and 2.) When the full-grown anuran oocyte is exposed to progesterone, a sequence of events is triggered, leading to the reinitiation of meiosis and formation of a fertilizable egg. This process is also a polar one. The basal portion of the germinal vesicle is the first to break down as the entire germinal vesicle moves toward the animal pole (3).Our knowledge of the physical forces that might produce such asymmetries and direct polar movements remains limited. The oocytes are randomly oriented in the ovary; thus gravity is not the causal agent. Strong evidence has now accumulated in the case of fucoid algae eggs that electrical currents and ion gradients are involved in transducing environmental asymmetries into cellular polarity (4-6). Electrical currents associated with growth and localization processes have also been shown to exist in insect oocytes (7), pollen tubes (8), and regenerating Acetabularia (9). There are also a few older surface potential measurements from which the existence of polar currents in animal eggs can be inferred (10). It is against this background that this investigation of animal-vegetal currents in Xenopus oocytes and eggs was begun.METHODS AND MATERIALS Sexually mature Xenopus laevls females were obtained from the South African Snake Farm, Fish Hoek, South Africa. To obtain oocytes, females were anesthetized by chilling in an ice bath, portions of their ovaries were removed, and the oocytes were then manually dissected from their follicles. In some cases, the remaining follicle cells were removed by a 5-min treatment with Pronase (50 ;tg per ml in Steinberg's solution).A vibrating platinum electrode was used to measure extracellular currents. T...

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Section: Resultsmentioning

confidence: 99%

Electrical currents through full-grown and maturing Xenopus oocytes.

Robinson¹

1979

Proc. Natl. Acad. Sci. U.S.A.

108

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“…To examine this possibility, the ATPase activity of both transporters was determined in salivary glands treated with 5-HT at a concentration known to be saturating in physiological assays (Berridge and Prince, 1972). Under these conditions, total ATPase activity within the homogenate increased from 22.3±6.0·nmol·P i·gland -1 ·h -1 to 31.5±5.4·nmol·Pi·gland -1 ·h -1 (Fig.·3B).…”

Section: Effect Of 5-ht On Atpase Activitymentioning

confidence: 99%

Distribution and serotonin-induced activation of vacuolar-type H+-ATPase in the salivary glands of the blowflyCalliphora vicina

Zimmermann

Dames

Walz

et al. 2003

Journal of Experimental Biology

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SUMMARYSecretory activity in blowfly salivary glands is activated by the hormone serotonin. We have investigated the distribution and activity of two cation pumps that are possibly involved with transepithelial ion transport, i.e. Na+/K+-ATPase and vacuolar-type H+-ATPase(V-ATPase). By immunofluorescence labelling of secretory cells,Na+/K+-ATPase was localized on the basolateral plasma membrane and V-ATPase on the highly folded apical membrane. Activities of both ATPases were probed in salivary gland homogenates by applying specific inhibitors for these ion pumps, namely ouabain and bafilomycin A1. In control glands, bafilomycin-A1-sensitive V-ATPase activity and ouabain-sensitive Na+/K+-ATPase activity accounted for 36% and 19%, respectively, of the total ATPase activity. V-ATPase activity increased approximately twofold after stimulation with serotonin, whereas Na+/K+-ATPase activity was not significantly affected. Biochemical assays provided evidence that the serotonin-induced activation of V-ATPase activity was accompanied by a recruitment of peripheral V1subunits from the cytosol to the plasma membrane, indicative of the assembly of V0V1 holoenzymes.These data show that a V-ATPase located in the apical plasma membranes of the secretory cells is a component of the apical `potassium pump' that has been identified previously by physiological approaches. The V-ATPase energizes the apical membrane and provides the primary driving force for fuelling a putative K+/nH+ antiporter and, thus, for fluid secretion. Serotonin-induced assembly of V0V1holoenzymes might constitute a regulatory mechanism for the control of pump activity.

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“…It has been well established that the binding of 5-HT to its receptors on blowfly salivary gland cells induces the production of cAMP and the formation of inositol trisphosphate, the latter resulting in a rise in cytosolic Ca 2ϩ concentration and an increase of Ca 2ϩ -dependent Cl Ϫ conductance (21)(22)(23)(24)(25)(26)(27)(28). Here, we focus on the question of whether the cAMP pathway carries the information regarding 5-HT receptor activation to the V-ATPase.…”

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confidence: 99%

cAMP regulates plasma membrane vacuolar-type H ⁺ -ATPase assembly and activity in blowfly salivary glands

Dames

Zimmermann²,

Schmidt³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Reversible assembly of the V0V1 holoenzyme from V0 and V1 subcomplexes is a widely used mechanism for regulation of vacuolar-type H ؉ -ATPases (V-ATPases) in animal cells. In the blowfly (Calliphora vicina) salivary gland, V-ATPase is located in the apical membrane of the secretory cells and energizes the secretion of a KCl-rich saliva in response to the hormone serotonin. We have examined whether the cAMP pathway, known to be activated by serotonin, controls V-ATPase assembly and activity. Fluorescence measurements of pH changes at the luminal surface of isolated glands demonstrate that cAMP, Sp-adenosine-3 ,5 -cyclic monophosphorothioate, or forskolin, similar to serotonin, cause VATPase-dependent luminal acidification. In addition, V-ATPasedependent ATP hydrolysis increases upon treatment with these agents. Immunofluorescence microscopy and pelleting assays have demonstrated further that V1 components become translocated from the cytoplasm to the apical membrane and V-ATPase holoenzymes are assembled at the apical membrane during conditions that increase intracellular cAMP. Because these actions occur without a change in cytosolic Ca 2؉ , our findings suggest that the cAMP pathway mediates the reversible assembly and activation of V-ATPase molecules at the apical membrane upon hormonal stimulus.regulation ͉ translocation ͉ secretion T he vacuolar-type H ϩ -ATPases (V-ATPases) are multisubunit heteromeric complexes that are organized into two domains, designated V 0 and V 1 (1-4). V 0 forms a membranespanning proton-translocating complex; in yeast, it is composed of at least five different subunits termed a, c, cЈ, cЉ, and d, of which subunit c binds bafilomycin A 1 , a specific inhibitor of V-ATPases (5-8). The V 1 sector is attached to the cytoplasmic side of the V 0 sector, consists of at least eight different subunits termed A-H, and contains catalytic and noncatalytic ATPbinding sites. V-ATPase is vital for almost every eukaryotic cell and fulfils a variety of functions. On intracellular acidic membrane systems, such as endosomes, lysosomes, and synaptic vesicles, these proton pumps are involved in protein sorting during biosynthetic and endocytotic pathways, zymogen activation, and transmitter uptake, respectively (3, 4). V-ATPase molecules in the plasma membrane of animal cells, especially on the apical plasma membrane of epithelial cells, contribute to intracellular pH homeostasis, extracellular acidification, or alkalinization, or they energize the plasma membrane for secondary transport processes (3, 4).In some cells, V-ATPase requires a considerable amount of energy. For reasons of economy, it is thus favorable if V-ATPase activity is adapted to the physiological needs of the cell. Several regulatory mechanisms have been identified (1,2,4,9). One of these is the reversible dissociation of the V 1 sector from the V 0 sector, as revealed by experiments performed in yeast, midgut epithelial cells of the tobacco hornworm Manduca sexta, mammalian dendritic cells, and renal epithelial cells (10-16). In th...

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Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5‐hydroxytryptamine.

Cited by 96 publications

References 9 publications

Electrical currents through full-grown and maturing Xenopus oocytes.

Electrical currents through full-grown and maturing Xenopus oocytes.

Distribution and serotonin-induced activation of vacuolar-type H+-ATPase in the salivary glands of the blowflyCalliphora vicina

cAMP regulates plasma membrane vacuolar-type H ⁺ -ATPase assembly and activity in blowfly salivary glands

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Membrane permeability changes during stimulation of isolated salivary glands of Calliphora by 5‐hydroxytryptamine.

Cited by 96 publications

References 9 publications

Electrical currents through full-grown and maturing Xenopus oocytes.

Electrical currents through full-grown and maturing Xenopus oocytes.

Distribution and serotonin-induced activation of vacuolar-type H+-ATPase in the salivary glands of the blowflyCalliphora vicina

cAMP regulates plasma membrane vacuolar-type H + -ATPase assembly and activity in blowfly salivary glands

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cAMP regulates plasma membrane vacuolar-type H ⁺ -ATPase assembly and activity in blowfly salivary glands