Membrane permeabilizers enhance biofortification of Brassica microgreens by interspecific transfer of metabolites from tea (Camellia sinensis)

Davosir, Dino; Šola, Ivana

doi:10.1016/j.foodchem.2023.136186

Cited by 6 publications

(3 citation statements)

References 64 publications

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“…Compared to the activity against α-amylase, the activity of the broccoli microgreen extracts against lipase was higher. We had previously already found such a result when we analyzed the effects of hot and cold water stress on broccoli [11] and interspecific metabolite transfer for the biofortification of broccoli [101]. HT did not change the potential of the broccoli microgreens to inhibit lipase activity; however, LT significantly improved it.…”

Section: Effects Of High and Low Growing Temperatures On Potential Of...mentioning

confidence: 74%

Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

Šola,

Gmižić,

Pinterić

et al. 2024

IJMS

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Climate change causes shifts in temperature patterns, and plants adapt their chemical content in order to survive. We compared the effect of low (LT) and high (HT) growing temperatures on the phytochemical content of broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) microgreens and the bioactivity of their extracts. Using different spectrophotometric, LC-MS/MS, GC-MS, and statistical methods, we found that LT increased the total phenolics and tannins in broccoli. The total glucosinolates were also increased by LT; however, they were decreased by HT. Soluble sugars, known osmoprotectants, were increased by both types of stress, considerably more by HT than LT, suggesting that HT causes a more intense osmotic imbalance. Both temperatures were detrimental for chlorophyll, with HT being more impactful than LT. HT increased hormone indole-3-acetic acid, implying an important role in broccoli’s defense. Ferulic and sinapic acid showed a trade-off scheme: HT increased ferulic while LT increased sinapic acid. Both stresses decreased the potential of broccoli to act against H2O2 damage in mouse embryonal fibroblasts (MEF), human keratinocytes, and liver cancer cells. Among the tested cell types treated by H2O2, the most significant reduction in ROS (36.61%) was recorded in MEF cells treated with RT extracts. The potential of broccoli extracts to inhibit α-amylase increased following both temperature stresses; however, the inhibition of pancreatic lipase was increased by LT only. From the perspective of nutritional value, and based on the obtained results, we conclude that LT conditions result in more nutritious broccoli microgreens than HT.

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Section: Effects Of High and Low Growing Temperatures On Potential Of...mentioning

confidence: 74%

Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

Šola,

Gmižić,

Pinterić

et al. 2024

IJMS

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“…The extraction of phenolic compounds from grapevine leaves was carried out with 70% (v/v) ethanol as a solvent . For extraction, a volume of 1 mL of 70% ethanol was mixed with 20 mg of the lyophilized powdered plant material.…”

Section: Methodsmentioning

confidence: 99%

Flavescence Dorée Strain-Specific Impact on Phenolic Metabolism Dynamics in Grapevine (Vitis vinifera) throughout the Development of Phytoplasma Infection

Davosir,

Šola,

Ludwig-Müller

et al. 2023

J. Agric. Food Chem.

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Flavescence doreé phytoplasma (FDp) is a phytopathogenic bacterium associated with Grapevine yellowS disease, which causes heavy damage to viticultural production. Epidemiological data revealed that some FDp strains appear to be more widespread and aggressive. However, there is no data on mechanisms underlying the variable pathogenicity among strains. In this research, we employed chromatographic and spectrophotometric techniques to assess how two strains of FDp influence the levels of grapevine phenolic compounds, which are frequently utilized as indicative markers of stress conditions. The results pointed to the upregulation of all branches of phenolic metabolism through the development of infection, correlating with the increase in antioxidative capacity. The more aggressive strain M54 induced stronger downregulation of phenolics' accumulation at the beginning and higher upregulation by the end of the season than the less aggressive M38 strain. These findings reveal potential targets of FDp effectors and provide the first functional demonstration of variable pathogenicity between FDp strains, suggesting the need for future comparative genomic analyses of FDp strains as an important factor in exploring the management possibilities of FDp.

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“…PCA is an unsupervised mode of data statistics that can restore the sample to the real state of metabolism. If there is an obvious trend of separation in the PCA score graph, it indicates that there is a significant difference in the composition of metabolites among the samples [25]. In the PCA plot of the four tea process method samples and the QC sample (Figure 2A), PC1 and PC2 are 22.3% and 14.7%, respectively.…”

Section: Differential Metabolite Analysis Based On Pcamentioning

confidence: 99%

Comparative Metabolomics Study of Four Kinds of Xihu Longjing Tea Based on Machine Fixing and Manual Fixing Methods

Cui,

Mao,

Zhao

et al. 2023

Foods

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China Xihu Longjing tea is famous for its good flavor and quality. However, information on its related metabolites, except for flavonoids, is largely deficient. Different processing methods for China Xihu Longjing tea fixing—by machines at both the first and second step (A1), first step by machine and second step by hand (A2), first step by hand and second step by machine (A3), and by hand at both the first and second step (A4)—were compared using a UHPLC–QE–MS-based metabolomics approach. Liquid chromatography–mass spectrometry was used to analyze the metabolic profiles of the processed samples. A total of 490 metabolites (3 alkaloids, 3 anthracenes, 15 benzene and substituted derivatives, 2 benzopyrans, 13 coumarins and derivatives, 128 flavonoids, 4 furanoid lignans, 16 glycosides and derivatives, 5 indoles and derivatives, 18 isocoumarins and derivatives, 4 chalcones and dihydrochalcones, 4 naphthopyrans, 3 nucleosides, 78 organic acids and derivatives, 55 organooxygen compounds, 5 phenols, 109 prenol lipids, 3 saccharolipids, 3 steroids and steroid derivatives, and 17 tannins) were identified. The different metabolic profiles were distinguished using PCA and OPLS-DA. There were differences in the types and contents of the metabolites, especially flavonoids, furanoid lignans, glycosides and derivatives, organic acids and derivatives, and organooxygen compounds. There was a positive correlation between flavonoid metabolism and amino acid metabolism. However, there was a negative correlation between flavonoid metabolism and amino acid metabolism, which had the same trend as prenol lipid metabolism and tannins. This study provides new valuable information regarding differences in the metabolite profile of China Xihu Longjing tea processed based on machine fixing and on manual fixing methods.

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Membrane permeabilizers enhance biofortification of Brassica microgreens by interspecific transfer of metabolites from tea (Camellia sinensis)

Cited by 6 publications

References 64 publications

Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

Adjustments of the Phytochemical Profile of Broccoli to Low and High Growing Temperatures: Implications for the Bioactivity of Its Extracts

Flavescence Dorée Strain-Specific Impact on Phenolic Metabolism Dynamics in Grapevine (Vitis vinifera) throughout the Development of Phytoplasma Infection

Comparative Metabolomics Study of Four Kinds of Xihu Longjing Tea Based on Machine Fixing and Manual Fixing Methods

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