Membrane progesterone receptors β and γ have potential as prognostic biomarkers of endometrial cancer

Sinreih, Maša; Knific, Tamara; Thomas, Peter; Grazio, Snježana Frković; Rižner, Tea Lanišnik

doi:10.1016/j.jsbmb.2018.01.011

Cited by 38 publications

(27 citation statements)

References 45 publications

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“…It has been recently reported that the expression of PAQR7 and the respective protein content are decreased in endometrial cancer compared to adjacent unaffected tissue [ 35 ]. In the present study, we also found a decrease in the mRNA expression and protein content of mPR α in the ectopic endometrium of women with endometriosis, suggesting a possible connection between the alterations in endometriosis and endometrial cancer, as previously proposed [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…PAQR7 expression is induced during the secretory phase of the menstrual cycle, whereas the expression of PAQR5 and PAQR9 is decreased during that phase [ 30 ]. In addition, PAQR7 and PAQR8 expression and the respective protein content are decreased in endometrial cancer compared to adjacent nonaffected endometrium, whereas mPR γ protein content is increased in endometrial cancer tissue [ 35 ]. To the best of our knowledge, it has not been demonstrated whether gene expression and protein content of mPRs are altered in ectopic lesions and eutopic endometrium of patients with endometriosis.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis

Vázquez-Martínez

Bello-Álvarez

Hermenegildo-Molina

et al. 2020

BioMed Research International

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Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of mPR members in the eutopic and ectopic endometrium of women with endometriosis. Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR6 (mPRδ) at mRNA and protein levels was evaluated by RT-qPCR and Western blot, whereas PAQR5 (mPRγ) gene expression was evaluated by RT-qPCR. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey’s multiple comparisons test with a confidence interval of 95 %. The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis

Vázquez-Martínez

Bello-Álvarez

Hermenegildo-Molina

et al. 2020

BioMed Research International

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“…Membrane progesterone receptors (mPRs) that belong to the progestin and adipoQ receptor (PAQR) family are putative non-classical progesterone signaling molecules [ 7 , 8 , 9 ]. To date, five of these molecules have been identified in humans: PAQR5 (mPRγ), PAQR6 (mPRδ), PAQR7 (mPRα), PAQR8 (mPRβ), and PAQR9 (mPRε) [ 10 ]. Differential expression of PAQR5 and PAQR8 was found in ovarian cystadenomas, borderline tumors, and carcinomas, suggesting that they have important roles in epithelial ovarian tumors [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Differential expression of PAQR5 and PAQR8 was found in ovarian cystadenomas, borderline tumors, and carcinomas, suggesting that they have important roles in epithelial ovarian tumors [ 11 ]. PAQR5 and PAQR8 were also supposed to be potential prognostic biomarkers of endometrial cancer [ 10 ]. In human glioblastoma cells, the activation of PAQR7 has been found to exhibit a promoting effect on cell proliferation and motility, implying its involvement in glioblastoma development [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

PAQR6 expression enhancement suggests a worse prognosis in prostate cancer patients

Zhe

Qin

et al. 2018

Open Life Sciences

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ObjectiveThis study aimed to evaluate the expression of progestin and adipoQ receptor family member VI (PAQR6, mPRδ) in prostate cancer and to explore its role in prostate cancer progression.MethodsPAQR6 mRNA expression was evaluated based on the data obtained from the TCGA database and the GEO database. The prognostic value of PAQR6 was explored by Kaplan-Meier analysis. To investigate the role of PAQR6, it was depleted by siRNA in DU145 cells. The effects of depleting PAQR6 on DU145 cell viability and migration were determined by CCK8 assay, colony formation assay, and wound healing assay, respectively. The activation of MEK and ERK were analyzed by western blot.ResultsPAQR6 mRNA expression was significantly up-regulated in prostate cancer tissues and correlated with lower survival rates (p=0.014). Furthermore, qPCR revealed that PAQR6 expression was elevated in DU145 and LNCaP cells compared with RWPE-2 cells. Depleting PAQR6 obviously suppressed DU145 cell proliferation and migration (p<0.01). In addition, the ratio of p-MEK/MEK and p-ERK/ERK was significantly reduced after silencing PAQR6 (p<0.01).ConclusionPAQR6 might play a facilitating role in prostate cancer development by regulating the MAPK signaling pathway. Moreover, it might serve as a potential predictor and therapeutic target in prostate cancer.

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“…PAQR7 expression is induced during the secretory phase of the menstrual cycle, whereas the expression of PAQR5 and PAQR9 is decreased during that phase [30]. In addition, PAQR7 and PAQR8 expression and the respective protein content are decreased in endometrial cancer compared to adjacent non-affected endometrium, whereas mPRγ protein content is increased in endometrial cancer tissue [35]. To the best of our knowledge, it has not been demonstrated whether gene expression and protein content of mPRs are altered in ectopic lesions and eutopic endometrium of patients with endometriosis.…”

Section: Introductionmentioning

confidence: 97%

Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

Vázquez-Martínez

Bello-Álvarez

Hermenegildo-Molina

et al. 2020

Preprint

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Background: Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by non-genomic mechanisms via membrane progesterone receptors that belong to the class II members of the progesterone and adipoQ receptor (PAQR) family. The aim of the present study was to evaluate the expression at mRNA and protein levels of PAQR family members in the eutopic and ectopic endometrium of women with endometriosis. Methods: Total RNA and total protein were isolated from control endometrium (17 samples), eutopic endometrium (17 samples), and ectopic endometrium (9 samples). The expression of PAQR7 ( mPRα ) , PAQR8 , (mPRβ) PAQR5 ( mPRγ ) , and PAQR6 ( mPRδ ) at mRNA and protein levels was evaluated by RT-qPCR and western blot. Statistical analysis between comparable groups was performed using one-way ANOVA followed by Tukey's multiple comparisons test with a confidence interval of 95%. Results: The analysis of gene expression showed that PAQR7 and PAQR5 expression was lower in both eutopic and ectopic endometrium as compared to the endometrium of women without endometriosis, whereas the expression of PAQR8 and PAQR6 was only reduced in eutopic endometrium. Furthermore, mPRα and mPRβ protein content was decreased in the ectopic endometrium of women with endometriosis. Conclusions: Our results demonstrate a decrease in the expression and protein content of mPRs in eutopic and ectopic endometrium of patients with endometriosis, which could contribute to the progesterone resistance observed in patients with this disease.

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Membrane progesterone receptors β and γ have potential as prognostic biomarkers of endometrial cancer

Cited by 38 publications

References 45 publications

Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis

Expression of Membrane Progesterone Receptors in Eutopic and Ectopic Endometrium of Women with Endometriosis

PAQR6 expression enhancement suggests a worse prognosis in prostate cancer patients

Expression of membrane progesterone receptors in eutopic and ectopic endometrium of women with endometriosis

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