Membrane remodelling in phosphorus‐deficient plants

Siebers, Meike; Dörmann, Peter; Hölzl, Georg

doi:10.1002/9781118958841.ch9

Cited by 23 publications

(36 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regeneration of galactolipids and sulfolipids using DAG is the next step of the lipid remodelling pathway (Figure ). During this step, different galactolipids (monogalactosyldiacylglycerol [MGDG] and digalactosyldiacylglycerol [DGDG]) and sulfolipids (sulfoquinovosyldiacylglycerol [SQDG]) are biosynthesized (Siebers et al, ). Genes involved in the synthesis of galactolipids ( MGD2 and DGD2 ) and sulfolipids ( SQD1 and SQD2 ) are upregulated in rice under Pi deficiency (Table , Jeong et al, ; Mehra et al, ).…”

Section: Membrane Lipid Remodelling: a Molecular Strategy To Drive P mentioning

confidence: 99%

“…The function of PLC is to hydrolyse phospholipids resulting in DAG and a phosphorylated head group, which is then further hydrolysed by acid phosphatase (APase) to release Pi (Stigter & Plaxton, 2015). PLD is involved in the formation of DAG and phosphatidic acid (PA; Stigter & Plaxton, 2015;Wang, 2000) as a result of cleaving the terminal phosphodiester bond of phospholipids (Siebers, Dörmann, & Hölzl, 2015). Then, PA can be further hydrolysed by a PA phosphatase (action is regulated by PAH genes) resulting in DAG formation and releasing Pi (Nakamura et al, 2005).…”

Section: Membrane Lipid Remodelling: a Molecular Strategy To Drive mentioning

confidence: 99%

See 1 more Smart Citation

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

Dissanayaka

Plaxton

Lambers

et al. 2018

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Orthophosphate (H PO , Pi) is an essential macronutrient integral to energy metabolism as well as a component of membrane lipids, nucleic acids, including ribosomal RNA, and therefore essential for protein synthesis. The Pi concentration in the solution of most soils worldwide is usually far too low for maximum growth of crops, including rice. This has prompted the massive use of inefficient, polluting, and nonrenewable phosphorus (P) fertilizers in agriculture. We urgently need alternative and more sustainable approaches to decrease agriculture's dependence on Pi fertilizers. These include manipulating crops by (a) enhancing the ability of their roots to acquire limiting Pi from the soil (i.e. increased P-acquisition efficiency) and/or (b) increasing the total biomass/yield produced per molecule of Pi acquired from the soil (i.e. increased P-use efficiency). Improved P-use efficiency may be achieved by producing high-yielding plants with lower P concentrations or by improving the remobilization of acquired P within the plant so as to maximize growth and biomass allocation to developing organs. Membrane lipid remodelling coupled with hydrolysis of RNA and smaller P-esters in senescing organs fuels P remobilization in rice, the world's most important cereal crop.

show abstract

Section: Membrane Lipid Remodelling: a Molecular Strategy To Drive P mentioning

confidence: 99%

Section: Membrane Lipid Remodelling: a Molecular Strategy To Drive mentioning

confidence: 99%

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

Dissanayaka

Plaxton

Lambers

et al. 2018

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…We propose that the major reason behind these protein changes may be a phospholipid to phosphorus-free lipid replacement in the PM of non-symbiotic roots because of decreased phosphate nutrition relative to AM plants . The response of membrane lipid composition to Pi starvation essentially may comprise the degradation of phospholipids to release Pi, and the synthesis of glycolipids as nonphosphorus lipids to substitute for those phospholipids (Siebers et al 2015). The role of phospholipase D in the breakdown of PM phospholipids during phosphate deficiency actually was established in oat and thale cress (Andersson et al 2005;Cruz-Ramírez et al 2006).…”

Section: Am-responsive Proteins As Related To Interface Biogenesis Anmentioning

confidence: 99%

The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis

Aloui¹,

Recorbet²,

Lemaître‐Guillier³

et al. 2017

Mycorrhiza

View full text Add to dashboard Cite

In arbuscular mycorrhizal (AM) roots, the plasma membrane (PM) of the host plant is involved in all developmental stages of the symbiotic interaction, from initial recognition to intracellular accommodation of intra-radical hyphae and arbuscules. Although the role of the PM as the agent for cellular morphogenesis and nutrient exchange is especially accentuated in endosymbiosis, very little is known regarding the PM protein composition of mycorrhizal roots. To obtain a global overview at the proteome level of the host PM proteins as modified by symbiosis, we performed a comparative protein profiling of PM fractions from Medicago truncatula roots either inoculated or not with the AM fungus Rhizophagus irregularis. PM proteins were isolated from root microsomes using an optimized discontinuous sucrose gradient; their subsequent analysis by liquid chromatography followed by mass spectrometry (MS) identified 674 proteins. Cross-species sequence homology searches combined with MS-based quantification clearly confirmed enrichment in PM-associated proteins and depletion of major microsomal contaminants. Changes in protein amounts between the PM proteomes of mycorrhizal and non-mycorrhizal roots were monitored further by spectral counting. This workflow identified a set of 82 mycorrhiza-responsive proteins that provided insights into the plant PM response to mycorrhizal symbiosis. Among them, the association of one third of the mycorrhiza-responsive proteins with detergent-resistant membranes pointed at partitioning to PM microdomains. The PM-associated proteins responsive to mycorrhization also supported host plant control of sugar uptake to limit fungal colonization, and lipid turnover events in the PM fraction of symbiotic roots. Because of the depletion upon symbiosis of proteins mediating the replacement of phospholipids by phosphorus-free lipids in the plasmalemma, we propose a role of phosphate nutrition in the PM composition of mycorrhizal roots.

show abstract

“…Pi incorporated in lipid membranes also represents an abundant and readily available source. Shortly after the onset of Pi deficiency, nutrient starvation leads to the replacement of phosphoglycerolipids, such as phosphatidyl-choline (PtdCho) and phosphatidyl-ethanolamine (PtdEth), by sulfolipids (sulfoquinovosyldiacylglycerol) and galactolipids (digalactosyldiacylglycerol) within the membranes (Misson et al, 2005;Nakamura, 2013;Siebers et al, 2015). The two latter lipid forms are devoid of Pi, which allows the cell to recycle this element into other pathways that require Pi.…”

mentioning

confidence: 99%

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

View full text Add to dashboard Cite

Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes. These observations suggest that the enzymes play a similar role in planta related to the recycling of polar heads from membrane lipids that is triggered during phosphate starvation. Altering the expression of the genes encoding these enzymes had no effect on lipid composition, possibly due to compensation by other lipid recycling pathways triggered during phosphate starvation. Furthermore, our results indicated that PPsPase1 and PECP1 do not influence phosphate homeostasis, since the inactivation of these genes had no effect on phosphate content or on the induction of molecular markers related to phosphate starvation. A combination of transcriptomics and imaging analyses revealed that PPsPase1 and PECP1 display a highly dynamic expression pattern that closely mirrors the phosphate status. This temporal dynamism, combined with the wide range of induction levels, broad expression, and lack of a direct effect on Pi content and regulation, makes PPsPase1 and PECP1 useful molecular markers of the phosphate starvation response.Plant growth is highly sensitive to a lack of phosphate (Pi); hence, the application of Pi fertilizers has become standard practice for high-throughput crop production (Cordell et al., 2009). Most phosphorus in the soil is not available to plants, as it is combined with other minerals or parts of organic compounds (Bieleski, 1973;Raghothama and Karthikeyan, 2005). Only a small fraction of soluble Pi (usually present at a concentration of ,10 mM in the soil) can be taken up by plants.Although growth is not optimal under limiting conditions, plants can withstand changing Pi concentrations within heterogeneous soils or in the external nutrient supply that can lead to reprogramming of their metabolism and architecture (Hammond et al., 2003;Péret et al., 2011;Plaxton and Tran, 2011). Root architecture can be modified to facilitate Pi uptake by favoring the development of lateral roots (at the expense of primary root elongation in many plants including Arabidopsis), increasing the density and length of root hairs, and limiting the development of aerial parts (López-Bucio et al., 2002;Svistoonoff et al., 2007;Gruber et al., 2013). Pi uptake mechanisms are enhanced at the root/soil interface, particularly through the stimulation of Pi transport activity (Mudge et al., 2002;Shin et al., 2004;Nussaume et al., 2011; Ayadi et al., 2015). In parallel, mobilization of Pi sources fr...

show abstract

Membrane remodelling in phosphorus‐deficient plants

Cited by 23 publications

References 116 publications

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

Molecular mechanisms underpinning phosphorus‐use efficiency in rice

The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

Contact Info

Product

Resources

About