Membrane Stability of Red Blood Cells in Diabetic Mice Treated with D-3-O-Methylchiroinositol

Nwaehujor, Chinaka O.; Onyeka, Asuzu; U, Asuzu Isaac

doi:10.12691/ajps-2-1-5

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…16 Mean corpuscular Fragility (MCF) is known to be a technique which enumerates the concentration of saline causing 50% haemolysis in erythrocytes. This elucidates how plants can maintain membrane stability against hypotonic solution induced stress.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Properties of ADJ6 and ITS Effect on Isolated Human Erythrocyte Ghost Membrane

Duraiswamy

Shanmugasundaram

Sasikumar

et al. 2016

FRA

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Introduction: Oxidative stress is one of the major causes for many adverse complications especially in disorders such as diabetes, cardiovascular diseases, cancer, etc. Many studies have proven that erythrocytes are affected during these conditions and oxidative stress plays a critical role in damaging the membrane integrity of erythrocytes by inducing lipid peroxidation causing instability to membrane in turn affecting the ion transport mechanisms. Objective and methods: The present study focuses to study the ameliorative effect of ADJ6 against oxidative stress on human erythrocytes. The membrane stabilization activity, Inhibition of lipid peroxi dation and the effect against Ouabain induced Na + K + ATPase activity was assessed. Further, in vitro antioxidant activity of ADJ6 was also determined. Results and Discussion: DPPH radical scavenging activity, metal chelating and H 2 O 2 radical scavenging activity were IC 50 =37.9 ± 0.22 µg/ml, IC 50 =53.95 ± 0.337 µg/ml and IC 50 =69.5 ± 0.628 µg/ml respectively. ADJ6 showed an EC 50 =90.83 ± 1.905 µg/ml against hypotonicity induced lysis of erythrocytes. H 2 O 2 induced Lipid peroxidation of ghost membrane was also inhibited by ADJ6 (IC 50 =84.5 ± 2.613 µg/ml). Ouabain induced sodium potassium ATPase activity increased with the treatment of ADJ6 in a dose dependent manner. This proves that ADJ6 is effective in stabilizing the membrane potential of erythrocyte membrane. The scavenging activity in vitro also proves that ADJ6 is effective against free radicals. Conclusion: Though exact mechanism hitherto unknown, it is evident that ADJ6 stabilizes erythrocyte membrane activity. Further studies will be conducted in order to prove the mechanism of action of ADJ6 against of free radicals.

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Section: Discussionmentioning

confidence: 99%