Membrane surface properties of reticulocytes from rats rendered severely anemic with phenylhydrazine as determined by partition in aqueous phase systems

Walter, Harry; Miller, Alexander; Krob, Eugene J.; Ascher, G.S.

doi:10.1016/0014-4827(72)90112-7

Cited by 24 publications

(13 citation statements)

References 15 publications

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“…The life span of a red cell in the rat is about 55 days (21). In the circulating blood of have varied inhibitory action on ALA-D activi ty, but it is not so strong as that of lead (5).…”

Section: Discussionmentioning

confidence: 99%

“…This is probably due to the differences in the reticulo cyte activity related to age. We have tried to separate human red cells according to their age using countercurrent distribution technique and measure ALA-D activity, but due to some of the chemicals used in the system, more than 50% of the activity was lost (21). Further work is in progress to isolate young and old red cells from human blood and measure ALA-D activ Apart from lead, other heavy metals like mercury, cadmium, silver, copper, etc., also…”

Section: Discussionmentioning

confidence: 99%

“…an adult rat, the percentage of reticulocytes is about 3 to 5, and the maturation of reticulo cytes takes about 24 h (21). When the animals are repeatedly injected with phenylhydrazine for 3-5 days, the circulating blood consists mainly of reticulocytes.…”

Section: Methodsmentioning

confidence: 99%

“…In order to study the correlation between the percentage of reticulocytes in the circulating blood and ALA-D, we have induced a high degree of reticulocytosis in rats by injecting phenylhydrazine. Phenylhydrazine is known to produce anemia when injected in animals (21). The toxic effect of phenylhydrazine is due to the benzene nucleus and not due to the hydrazine part of the molecule.…”

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confidence: 99%

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Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Abdulla¹,

Svensson²

1978

Enzyme

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δ-aminolevulinic acid dehydratase activity was measured in the blood of rats poisoned by successive injections of phenylhydrazine (1 ml, 35 mmol). After 5 days of successive injections, more than 90% of the circulating blood cells consisted of reticulocytes and the enzyme activity also increased successively and reached a maximum corresponding to the number of reticulocytes. Thus ô-aminolevulinic acid dehydratase activity measured in the peripheral blood is mainly due to the percentage of circulating reticulocytes.

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“…The life span of a red cell in the rat is about 55 days (21). In the circulating blood of have varied inhibitory action on ALA-D activi ty, but it is not so strong as that of lead (5).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Abdulla¹,

Svensson²

1978

Enzyme

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“…Groups I and III were fed a normal diet and were injected with saline (subcutaneously) for 4 consecutive days, whereas groups II and IV were fed a low iron diet and received a daily subcutaneous injection of PH at a dose level of 30 mg/kg body weight for 4 days. This dose of PH is known to produce anemia when injected in mice, and does not produce any other apparent toxic manifestation in the treated mice (Walter et al 1972). Animals of all the groups received topical application of DMBA (40 lg DMBA as 150 ll per mouse) under subdued light, 24 h after the last injection of saline or PH.…”

Section: Treatment Of Animals and Tumor Studiesmentioning

confidence: 99%

Low iron diet retards 12- O -tetradecanoyl phorbol-13-acetate-mediated tumor promotion in murine skin

Bhasin

Kauser

Athar

2004

Archives of Toxicology

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Recently, we have shown that low iron state reduces benzoyl peroxide-mediated tumor promotion in murine skin. To further test the dependence of iron on skin tumor development, we assessed the effect of a low iron diet on 12- O-tetradecanoyl phorbol-13-acetate (TPA)-mediated tumor promotion in murine skin. Female Swiss albino mice were fed on a low iron diet and initiated with a single dose of 7,12-dimethylbenz[ a]anthracene (DMBA, 40 microg as 150 microl per mouse) and promoted with TPA (2.5 microg as 200 microl per mouse, twice-weekly application for 20 weeks). The appearance of the first papilloma and the number of tumors (papillomas and carcinomas) per mouse were recorded weekly. We observed a decrease in tumor incidence (papillomas and carcinomas) and number of tumors per mouse in TPA-promoted mice fed on low iron diet (0.23 mg Fe/kg diet) compared to normal mice fed on balanced mouse chow (1.70 mg Fe/kg diet). The total iron consumption per day by mice being fed on balanced mouse chow was 340-400 microg Fe/kg body weight, and the total iron consumption per day by mice being fed on low iron diet was 45-55 microg Fe/kg body weight. The number of papillomas per mouse was 45% lower and the conversion of papillomas to carcinomas was about 20% lower in mice fed a low iron diet. The tumor size was also smaller in mice being fed on low iron diet. TPA treatment resulted in decreases in the activities of antioxidant enzymes and depletion in the level of epidermal reduced glutathione (GSH). Feeding mice on low iron diet along with TPA treatment resulted in approximately 50-75% recovery of the depleted levels of GSH and antioxidant enzymes. In addition, TPA-mediated induction of biological markers of tumor promotion, viz. ornithine decarboxylase activity and [(3)H]thymidine incorporation into cutaneous DNA, was about 60% lower in TPA-treated mice fed on low iron diet than in normal mice treated with TPA. Cutaneous iron levels were also lower in mice fed on low iron diet than in mice fed on normal diet. Histopathological sections of the skin portion adjoining tumors showed a lower degree of epidermal hyperplasia and lesser infiltration of inflammatory cells in the dermis, and absence of hyperkeratosis in mice fed on low iron diet. Thus, in this study we observe that the tumor promoting potential of TPA is reduced in mice fed on low iron diet, which is also accompanied by lesser inflammatory changes in the skin of tumor-bearing mice fed on low iron diet.

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Pyruvate kinase activity and response to allosteric effectors in rat erythrocytes and reticulocytes fractionated by multiple partitioning in aqueous two‐phase systems

Pinilla

Rodriguez-Horche

Luque

1987

Cell Biochemistry & Function

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Specific activity of pyruvate kinase decreases as the age of rat erythrocytes increases in fractions obtained by counter-current distribution in dextran-polyethylene glycol biphasic systems; the enzyme is inhibited by ATP and activated by fructose-1,6-bisphosphate at low phosphoenol pyruvate concentrations. Specific activity does not change in fractions from greater than 95 per cent-rich reticulocytes (anaemic rats); the enzyme is inhibited by ATP but not activated by fructose-1,6-bisphosphate. These results can be explained on the basis of different pyruvate kinase isozymes and suggest that decrease in activity is not affecting regulatory properties during erythrocytes aging.

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Membrane surface properties of reticulocytes from rats rendered severely anemic with phenylhydrazine as determined by partition in aqueous phase systems

Cited by 24 publications

References 15 publications

Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Low iron diet retards 12- O -tetradecanoyl phorbol-13-acetate-mediated tumor promotion in murine skin

Pyruvate kinase activity and response to allosteric effectors in rat erythrocytes and reticulocytes fractionated by multiple partitioning in aqueous two‐phase systems

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