Membrane-tethered transcription factors provide a connection between stress response and developmental pathways

Slabaugh, Erin; Brandizzí, Federica

doi:10.4161/psb.6.8.16047

Cited by 13 publications

(11 citation statements)

References 19 publications

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“…As mentioned above, MTFs play critical roles in environmental stress and cellular ER stress responses. They help plants to cope with unfavourable growth conditions by regulating gene expression with their transcriptional activity [ 56 ]. Extending our understanding of the molecular mechanisms underlying how intracellular movement of MTFs is organized may provide us with an advantage by enabling plants to increase their stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

The nuclear transportation routes of membrane-bound transcription factors

Liu

Fan

et al. 2018

Cell Commun Signal

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Membrane-bound transcription factors (MTFs) are transcription factors (TFs) that are anchored in membranes in a dormant state. Activated by external or internal stimuli, MTFs are released from parent membranes and are transported to the nucleus. Existing research indicates that some plasma membrane (PM)-bound proteins and some endoplasmic reticulum (ER) membrane-bound proteins have the ability to enter the nucleus. Upon specific signal recognition cues, some PM-bound TFs undergo proteolytic cleavage to liberate the intracellular fragments that enter the nucleus to control gene transcription. However, lipid-anchored PM-bound proteins enter the nucleus in their full length for depalmitoylation. In addition, some PM-bound TFs exist as full-length proteins in cell nucleus via trafficking to the Golgi and the ER, where membrane-releasing mechanisms rely on endocytosis. In contrast, the ER membrane-bound TFs relocate to the nucleus directly or by trafficking to the Golgi. In both of these pathways, only the fragments of the ER membrane-bound TFs transit to the nucleus. Several different nuclear trafficking modes of MTFs are summarized in this review, providing an effective supplement to the mechanisms of signal transduction and gene regulation. Moreover, targeting intracellular movement pathways of disease-associated MTFs may significantly improve the survival of patients.

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Section: Discussionmentioning

confidence: 99%

The nuclear transportation routes of membrane-bound transcription factors

Liu

Fan

et al. 2018

Cell Commun Signal

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“…This makes the ER an important sensor of cellular stress as the accumulation of unfolded proteins is first detected by the ER. Membrane‐tethered transcription factors (MTTFs) residing at the ER membrane play important roles in ER stress perception and regulation of genes involved in stress relief and cell death in mammalian and plant systems (Slabaugh & Brandizzi, ). Membrane tethering provides spatial regulation of transcription factor activity, as MTTFs must be removed from the membrane before the transcription factor domain can translocate to the nucleus (Slabaugh & Brandizzi, ).…”

Section: Discussionmentioning

confidence: 99%

An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter‐binding protein (SBP) transcription factors that exhibit pro‐cell death characteristics

2018

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Despite the importance of proper cell death regulation across broad evolutionary distances, an understanding of the molecular machinery underpinning this fundamental process in plants remains largely elusive. This is despite its critical importance to development, homeostasis, and proper responses to stress. The identification of endogenous plant regulators of cell death has been hindered by the fact that many core regulators of cell death in animals are absent in plant genomes. Remarkably, numerous studies have shown that the ectopic expression of animal prosurvival genes in plants can suppress cell death imposed by many stresses. In this study, we capitalize on the ectopic expression of one of these animal prosurvival genes, an inhibitor of apoptosis from Spodoptera frugiperda (SfIAP), to identify novel cell death regulators in plants. A yeast two-hybrid assay was conducted using SfIAP as bait to screen a tomato cDNA library. This screen identified several transcription factors of the SQUAMOSA promoter-binding protein (SBP) family as potential SfIAP binding partners. We confirmed this interaction in vivo for our top two interactors, SlySBP8b and SlySBP12a, using coimmunoprecipitation.Interestingly, overexpression of SlySBP8b and SlySBP12a induced cell death in Nicotiana benthamiana leaves. Overexpression of these two transcription factors also induced the accumulation of reactive oxygen species and enhanced the growth of the necrotrophic pathogen Alternaria alternata. Fluorescence microscopy confirmed the nuclear localization of both SlySBP8b and SlySBP12a, while SlySBP12a was also localized to the ER membrane. These results suggest a prodeath role for SlySBP8b and SlySBP12a and implicate ER membrane tethering as a means of regulating SlySBP12a activity.

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“…This makes the ER an important sensor of cellular stress as the accumulation of unfolded proteins is first detected by the ER. Membrane-tethered transcription factors (MTTFs) residing at the ER membrane play important roles in ER stress perception and regulation of genes involved in stress relief and cell death in mammalian and plant systems (Slabaugh and Brandizzi, 2011). Membrane tethering provides spatial regulation of transcription factor activity, as MTTFs must be removed from the membrane before the transcription factor domain can translocate to the nucleus (Slabaugh and Brandizzi, 2011).…”

Section: Discussionmentioning

confidence: 99%

An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter binding protein (SBP) transcription factors that exhibit pro-cell death characteristics

Kessens

Sorensen

Kabbage

2018

Preprint

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Highlights: SBP transcription factors SlySBP8b and SlySBP12a from tomato interact with an insect inhibitor of apoptosis protein (SfIAP). Both exhibit pro-cell death characteristics while SlySBP12a activity may be regulated through ER membrane tethering. AbstractDespite the functional conservation of programmed cell death (PCD) across broad evolutionary distances, an understanding of the molecular machinery underpinning this fundamental program in plants remains largely elusive. This is despite its critical importance to development, homeostasis, and proper responses to stress. Progress in plant PCD has been hindered by the fact that many core regulators of animal PCD are absent in plant genomes. Remarkably, numerous studies have shown that the ectopic expression of animal anti-PCD genes in plants can suppress cell death imposed by many stresses. In this study, we capitalize on the ectopic expression of an insect inhibitor of apoptosis (SfIAP) to identify novel cell death regulators in plants. A yeast two-hybrid assay was conducted using SfIAP as bait to screen a tomato cDNA library. This screen identified several transcription factors of the SQUAMOSA promoter binding protein (SBP) family as potential SfIAP binding partners. We confirmed this interaction in vivo for our top two interactors, SlySBP8b and SlySBP12a, using coimmunoprecipitation. Interestingly, overexpression of SlySBP8b and SlySBP12a induced spontaneous cell death in Nicotiana benthamiana leaves. Overexpression of these two transcription factors also induced the accumulation of reactive oxygen species and enhanced the growth of the necrotrophic pathogen Alternaria alternata. Fluorescence microscopy confirmed the nuclear localization of both SlySBP8b and SlySBP12a, while SlySBP12a was also localized to the ER membrane. These results support a pro-death role for SlySBP8b and SlySBP12a and provide potential targets that can be utilized to improve stress tolerance in crop plants.3

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Membrane-tethered transcription factors provide a connection between stress response and developmental pathways

Cited by 13 publications

References 19 publications

The nuclear transportation routes of membrane-bound transcription factors

The nuclear transportation routes of membrane-bound transcription factors

An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter‐binding protein (SBP) transcription factors that exhibit pro‐cell death characteristics

An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter binding protein (SBP) transcription factors that exhibit pro-cell death characteristics

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