2000
DOI: 10.1128/mmbr.64.1.13-33.2000
|View full text |Cite
|
Sign up to set email alerts
|

Membrane Topology and Insertion of Membrane Proteins: Search for Topogenic Signals

Abstract: SUMMARY Integral membrane proteins are found in all cellular membranes and carry out many of the functions that are essential to life. The membrane-embedded domains of integral membrane proteins are structurally quite simple, allowing the use of various prediction methods and biochemical methods to obtain structural information about membrane proteins. A critical step in the biosynthetic pathway leading to the folded protein in the membrane is its insertion into the lipid bilayer. Understandi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

7
169
0
2

Year Published

2002
2002
2012
2012

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 178 publications
(179 citation statements)
references
References 213 publications
(259 reference statements)
7
169
0
2
Order By: Relevance
“…The PE-dependent topological organization of PheP was shown through differences in accessibility by membrane-impermeable sulfhydryl reagents to single Cys replacements in the extramembrane domains connecting TMs. The advantage of this technique is that modification is restricted to a single amino acid residue that is less likely to disturb the overall protein structure (29). This idea was also supported by the maintenance of PheP function in cells with natural phospholipid composition.…”
Section: Fig 4 Determination Of Phep Topology In Pe-containing (A)mentioning
confidence: 52%
“…The PE-dependent topological organization of PheP was shown through differences in accessibility by membrane-impermeable sulfhydryl reagents to single Cys replacements in the extramembrane domains connecting TMs. The advantage of this technique is that modification is restricted to a single amino acid residue that is less likely to disturb the overall protein structure (29). This idea was also supported by the maintenance of PheP function in cells with natural phospholipid composition.…”
Section: Fig 4 Determination Of Phep Topology In Pe-containing (A)mentioning
confidence: 52%
“…2B) (37). In contrast, whereas the yeast ortholog (PEM2) is proposed to be similarly polytopic, one portion of the yeast protein contains a hydrophobic stretch of 31 amino acids, which, intriguingly, is the minimum length re- Endoproteinase Lys-C cleavage sites are denoted by arrows, and the length of cleavage fragments generated from proteolysis at each site, as measured from the N-terminal HA-tagged epitope, are indicated in kDa.…”
Section: Discussionmentioning
confidence: 99%
“…We employed an experimental system that has been widely applied to the topology analysis of plasma membrane proteins (Kast et al, 1998;van Geest et al, 2000), but here applied uniquely to a membrane protein on an intracellular organelle. Insertion of a small HA-tag was expected to have the least influence on the cellular behavior of OA1-GFP.…”
Section: Discussionmentioning
confidence: 99%