Membrane?wall attachments in plasmolysed plant cells

Lang, Ingeborg; Barton, Deborah; Overall, Robyn L.

doi:10.1007/s00709-004-0062-6

Cited by 42 publications

(38 citation statements)

References 51 publications

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“…S2). Consistent with the previous reports (Lemoine et al 1996;Kühn et al 1997;Schmitt et al 2008), NtSUT1 seemed to be localized at the plasma membrane, since NtSUT1-GFP fluorescence, but not GFP fluorescence, was observed at the Hechtian strands, a part of the plasma membrane connected to the cell wall, in the plasmolyzed cells (Lang et al 2004;Sasaki et al 2010).…”

Section: Resultssupporting

confidence: 92%

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sameeullah

Sasaki

Yamamoto

2013

Soil Science and Plant Nutrition

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The role of plasma membrane-localized sucrose transporter (NtSUT1) was investigated using cultured tobacco cell (Nicotiana tabacum L.) line BY-2. The wild type (WT) cells were first transformed with the NtSUT1 gene or its fragments cloned from tobacco cell line SL to form the over-expression (OX) and suppression (RNAi) cell lines, respectively. Using OX and RNAi transgenics, the role of NtSUT1 in growth capacity of actively growing cells and in aluminum (Al)-treated cells was examined. During the logarithmic phase of growth in nutrient medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), both the rate of sucrose uptake measured with radio-tracer and the content of soluble sugars were higher in OX and lower in RNAi cell lines compared to WT. Overall, the content of soluble sugars negatively correlated with the time necessary for doubling mass (fresh weight). When cells were treated without (control) or with Al in a simple medium containing calcium, sucrose and 2-(N-morpholino)ethanesulfonic acid (MES; pH 5.0) for up to 18 h, the expression of NtSUT1 under its native promoter, or under the control of strong constitutive cauliflower mosaic virus (CaMV) 35S promoter, was strongly dependent on the presence of 2,4-D. Thereafter, the cells were preferentially treated in the presence of 2,4-D. During 6 h after a start of the control treatment, sucrose uptake rates were, compared to WT, slightly higher and lower in OX and RNAi lines respectively. The addition of Al reduced the sucrose uptake rates of OX and WT to the level of RNAi line, indicating that Al inhibits sucrose uptake via NtSUT1. During the post-Al culture of control and Al-treated cells in a nutrient medium, sucrose uptake rates were much higher in OX compared to WT and RNAi lines, which closely and positively correlated with the growth capacity of the cells. Judging from the growth capacity of Al-treated cells relative to that of control cells, OX cells were more tolerant to Al than WT and RNAi. In summary, we conclude that over-expression of NtSUT1 confers higher growth capacity in actively growing cells as well as in Al-treated cells.

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Section: Resultssupporting

confidence: 92%

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sameeullah

Sasaki

Yamamoto

2013

Soil Science and Plant Nutrition

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“…S3). Observation of YFPT CESA6-labeled complexes remaining at the plant cell wall would be consistent with the model proposed by Lang et al (2004) whereby Hechtian strands are tethered to the cellulose microfibril array via CESA rosettes in the plasma membrane. Pretreatment of Arabidopsis seedlings with DCB (Supplemental Fig.…”

supporting

confidence: 69%

Nonmotile Cellulose Synthase Subunits Repeatedly Accumulate within Localized Regions at the Plasma Membrane in Arabidopsis Hypocotyl Cells following 2,6-Dichlorobenzonitrile Treatment

et al. 2007

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“…4D). These so called "Hechtian strands" are connections between the plasma membrane and wall (43,44) and indicate that the fluorescent signal from the MtPT3:GFP is associated with the plasma membrane (45). The same pattern of fluorescence was observed for MtPT5: GFP (data not shown).…”

Section: Resultsmentioning

confidence: 55%

Closely Related Members of the Medicago truncatula PHT1 Phosphate Transporter Gene Family Encode Phosphate Transporters with Distinct Biochemical Activities

Liu

Versaw

Pumplin

et al. 2008

Journal of Biological Chemistry

119

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Phosphorus is one of the essential mineral nutrients required by all living cells. Plants assimilate phosphate (P i ) from the soil, and their root systems encounter tremendous variation in P i concentration, both temporally and spatially. Genome sequence data indicate that plant genomes contain large numbers of genes predicted to encode P i transporters, the functions of which are largely unexplored. Here we present a comparative analysis of four very closely related P i transporters of the PHT1 family of Medicago truncatula. Based on their sequence similarity and locations in the genome, these four genes probably arose via recent gene duplication events, and they form a small subfamily within the PHT1 family. The four genes are expressed in roots with partially overlapping but distinct spatial expression patterns, responses to P i and expression during arbuscular mycorrhizal symbiosis. The proteins are located in the plasma membrane. Three members of the subfamily, MtPT1, MtPT2, and MtPT3, show low affinities for P i . MtPT5 shares 84% amino acid identity with MtPT1, MtPT2, and MtPT3 but shows a high affinity for P i with an apparent K m in yeast of 13 M. Sequence comparisons and protein modeling suggest that amino acid residues that differ substantially between MtPT5 and the other three transporters are clustered in two regions of the protein. The data provide the first clues as to amino acid residues that impact transport activity of plant P i transporter proteins.Phosphorus (P) is required by all organisms and is an essential component of cellular macromolecules, energy transfer reactions, and cellular metabolism (1). Plants acquire P as phosphate (P i ) from the soil and uptake into the roots occurs either directly through the root epidermal cells, or indirectly through arbuscular mycorrhizal (AM) 3 fungi with which most plants form symbiotic associations (2-5). Both the initial uptake and subsequent distribution of P i to cells throughout the plant require the activity of membrane transport proteins, and a combination of experimental evidence and genome sequence analyses indicate that plants contain a wide variety of P i transporter genes. Furthermore, the different P i transporter gene families are themselves composed of multiple members (2, 6). Current data suggest that members of the PHT1 P i transporter family mediate transfer of P i into cells, whereas members of the PHT2, PHT3, PHT4, and pPT families are involved in P i transfer across internal cellular membranes and organelle membranes (7-10).Members of the PHT1 P i transporter gene family have been identified from a wide range of plant species including Arabidopsis, rice, Medicago truncatula, and tomato (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Many of these transporters are expressed in roots and show elevated transcript levels during growth in low P i conditions. From the Arabidopsis and rice whole genome sequences, the full extent of the PHT1 transporter families is revealed and these species contain 9 and 13 members, respectively. Eight of the...

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Membrane?wall attachments in plasmolysed plant cells

Cited by 42 publications

References 51 publications

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Sucrose transporter NtSUT1 confers aluminum tolerance on cultured cells of tobacco (Nicotiana tabacumL.)

Nonmotile Cellulose Synthase Subunits Repeatedly Accumulate within Localized Regions at the Plasma Membrane in Arabidopsis Hypocotyl Cells following 2,6-Dichlorobenzonitrile Treatment

Closely Related Members of the Medicago truncatula PHT1 Phosphate Transporter Gene Family Encode Phosphate Transporters with Distinct Biochemical Activities

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