Membranes of unification

Nick, Peter

doi:10.1007/s00709-016-1050-3

Cited by 10 publications

(12 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many previous studies, including our studies, have shown that the TaGAPC1 was up-regulated under certain abiotic stresses in Chinese Spring Triticum aestivum [ 23 ]. Thus, in order to investigate the function and stress response mechanism of TaGApC2 , TaGApC2-6A/6B/6D were cloned in this study.…”

Section: Introductionmentioning

confidence: 85%

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Zhang

Yang

2020

IJMS

View full text Add to dashboard Cite

Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase in cytoplasm (GAPCs) have remained little investigated in wheat subjected to adverse environmental conditions. In this study, we cloned and characterized a GAPC isoform TaGAPC2 in wheat. Over-expression of TaGApC2-6D in Arabidopsis led to enhanced root length, reduced reactive oxygen species (ROS) production, and elevated drought tolerance. In addition, the dual-luciferase assays showed that TaWRKY28/33/40/47 could positively regulate the expression of TaGApC2-6A and TaGApC2-6D. Further results of the yeast two-hybrid system and bimolecular fluorescence complementation assay (BiFC) demonstrate that TaPLDδ, an enzyme producing phosphatidic acid (PA), could interact with TaGAPC2-6D in plants. These results demonstrate that TaGAPC2 regulated by TaWRKY28/33/40/47 plays a crucial role in drought tolerance, which may influence the drought stress conditions via interaction with TaPLDδ. In conclusion, our results establish a new positive regulation mechanism of TaGAPC2 that helps wheat fine-tune its drought response.

show abstract

Section: Introductionmentioning

confidence: 85%

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Zhang

Yang

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…The grapevine genome contains a family of 32 preliminarily annotated STS genes [ 24 ], but their precise function and the regulatory mechanism of each individual STS gene in response to biotic stresses remains unclear. Previous studies have shown that berries of the Chinese wild grapevine, V. amurensis cv.Tonghua-3, express multiple STS genes and accumulate resveratrol to high levels during development, and that resveratrol concentration is tightly correlated with expression of a specific STS gene, STS19 [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of the Grape VaSTS19 Gene in Arabidopsis Improves Resistance to Powdery Mildew and Botrytis cinerea but Increases Susceptibility to Pseudomonas syringe pv Tomato DC3000

Wang

et al. 2017

IJMS

View full text Add to dashboard Cite

Stilbene synthase (STS) is a key enzyme that catalyzes the biosynthesis of resveratrol compounds and plays an important role in disease resistance. The molecular pathways linking STS with pathogen responses and their regulation are not known. We isolated an STS gene, VaSTS19, from a Chinese wild grape, Vitis amurensis Rupr. cv. “Tonghua-3”, and transferred this gene to Arabidopsis. We then generated VaSTS19-expressing Arabidopsis lines and evaluated the functions of VaSTS19 in various pathogen stresses, including powdery mildew, B. cinerea and Pseudomonas syringae pv. tomato DC3000 (PstDC3000). VaSTS19 enhanced resistance to powdery mildew and B. cinerea, but increased susceptibility to PstDC3000. Aniline blue staining revealed that VaSTS19 transgenic lines accumulated more callose compared to nontransgenic control plants, and showed smaller stomatal apertures when exposed to pathogen-associated molecular patterns (flagellin fragment (flg22) or lipopolysaccharides (LPS)). Analysis of the expression of several disease-related genes suggested that VaSTS19 expression enhanced defense responses though salicylic acid (SA) and/or jasmonic acid (JA) signaling pathways. These findings provide a deeper insight into the function of STS genes in defense against pathogens, and a better understanding of the regulatory cross talk between SA and JA pathways.

show abstract

“…Although, a major proportion of transcripts for WDR proteins in W. somnifera was associated with regulation of growth and development related processes 2 , 3 , 52 , the transcripts were additionally observed to be associated with secondary metabolism indicating such possible dual role of these regulatory proteins in W. somnifera (Figs 5 , 6 Table S4 ). Role of WRKY proteins in W. somnifera has earlier been established with respect to secondary metabolic pathway related genes 14 , therefore the results from this study are more emphatic to their abundance in numbers and corresponding domain of regulatory effects. However, the study on role of WDR proteins namely WsLWD1 and WsWUSCHEL analyzed ab initio for the plant.…”

Section: Discussionmentioning

confidence: 82%

“…However, the study on role of WDR proteins namely WsLWD1 and WsWUSCHEL analyzed ab initio for the plant. The observed maximum expression of these TFs of WDR family in berry suggested their association with organ specific growth and development 2 , 3 , 14 , 52 , 69 (Figs 5 , 6 , 7 ). Tissues transiently transformed with these TFs, showed enhanced withanolide content confirming the additional role of WDR 52 – 68 proteins in secondary metabolism 44 , 48 .Thus, the study demonstrates that the two WDR proteins have regulatory role in both organ development processes as well as secondary metabolite accumulation in W. somnifera .…”

Section: Discussionmentioning

confidence: 87%

“…Current status of information regarding TFs in W. somnifera is almost limited to WRKY genes for which transcriptional regulation of pathway genes has been observed due to higher levels of corresponding gene transcripts 14 . Although, biosynthetic pathways for the major secondary metabolites of W. somnifera have previously been characterized, 9 – 11 , 15 – 18 the insights into their underlying regulatory mechanisms remain chiefly unexplored.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcription factor repertoire in Ashwagandha (Withania somnifera) through analytics of transcriptomic resources: Insights into regulation of development and withanolide metabolism

Tripathi

Sangwan

Narnoliya

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Transcription factors (TFs) are important regulators of cellular and metabolic functions including secondary metabolism. Deep and intensive RNA-seq analysis of Withania somnifera using transcriptomic databases provided 3532 annotated transcripts of transcription factors in leaf and root tissues, belonging to 90 different families with major abundance for WD-repeat (174 and 165 transcripts) and WRKY (93 and 80 transcripts) in root and leaf tissues respectively, followed by that of MYB, BHLH and AP2-ERF. Their detailed comparative analysis with Arabidopsis thaliana, Capsicum annum, Nicotiana tabacum and Solanum lycopersicum counterparts together gave interesting patterns. However, no homologs for WsWDR representatives, LWD1 and WUSCHEL, were observed in other Solanaceae species. The data extracted from the sequence read archives (SRA) in public domain databases were subjected to re-annotation, re-mining, re-analysis and validation for dominant occurrence of WRKY and WD-repeat (WDR) gene families. Expression of recombinant LWD1 and WUSCHEL proteins in homologous system led to enhancements in withanolide content indicating their regulatory role in planta in the biosynthesis. Contrasting expression profiles of WsLWD1 and WsWUSCHEL provided tissue-specific insights for their participation in the regulation of developmental processes. The in-depth analysis provided first full-spectrum and comparative characteristics of TF-transcripts across plant species, in the perspective of integrated tissue-specific regulation of metabolic processes including specialized metabolism.

show abstract

Membranes of unification

Cited by 10 publications

References 4 publications

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants

Expression of the Grape VaSTS19 Gene in Arabidopsis Improves Resistance to Powdery Mildew and Botrytis cinerea but Increases Susceptibility to Pseudomonas syringe pv Tomato DC3000

Transcription factor repertoire in Ashwagandha (Withania somnifera) through analytics of transcriptomic resources: Insights into regulation of development and withanolide metabolism

Contact Info

Product

Resources

About