Membranotropic effect of low-intensity laser radiation of the blood

Abstract

We have examined the effect of a low-intensity laser radiation (wavelength 630 nm, beam power 1 mW) on the aggregability of blood platelets, acidic tolerance of elythrocytes, and the structure of their membranes. Laser radiation suppressed platelet aggregability induced by ATP, epinephrine, collagen, platelet activating factor, and fibrinogen and enhanced the resistance of the erythrocytes. It decreased the lnicroviscosity of lipid bilayer and of the membrane protein-lipid contact regions and slowed down lipid peroxidation. These findings attest to a direct membranotropic effect of laser radiation, which is particularly strong in the protein-lipid contact regions.

Low-level light therapy reduces platelet destruction during extracorporeal circulation

“…LLLT inhibits PLT activation by such agonists as ADP, collagen, epinephrine, ristocetin, PAF, fibrinogen, adrenaline, ristomycin, and TRAP 17 , 37 – 39 . As such, irradiation most likely disrupts the activation processes of the coagulation system at a level common to all receptors.…”

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

“…The reduction in spherical (activated) cells was accompanied by increased discoidal (non-activated) cells. This proves a decrease in the degree of their activation in the population of PLTs exposed to red light 34 , 35 .…”

“…Thus, blood clotting is reduced and the clot formation time is extended; the hypercoagulation syndrome is formed 33 . At the same time, no change in the total PLT count was observed 34 , 35 , 38 , but there has been a redistribution of the population’s shape. The reduction in spherical (activated) cells was accompanied by increased discoidal (non-activated) cells.…”

“…Finally, R/NIR radiation causes biochemical changes in the membrane of PLTs in the lipid phase 35 , 40 , 41 . The authors suggest that the observed changes in the microviscosity of the lipid bilayer, also in the contact regions of lipids and proteins, may cause the activation of the cellular regulatory system and the synthesis of secondary messengers 40 .…”