Memory B-Cell Development After Asymptomatic or Mild Symptomatic SARS-CoV-2 Infection

Kato, Yu; Bloom, Nathaniel I.; Sun, Peifang; Balinsky, Corey A.; Qiu, Qi; Cheng, Ying; Jani, Vihasi; Schilling, Megan A.; Goforth, Carl; Weir, Dawn L.; Ramos, Irene; Sealfon, Stuart C.; Letizia, Andrew G.; Crotty, Shane

doi:10.1093/infdis/jiac319

Cited by 7 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This confirms that both groups generated neutralizing antibodies even without being aware that they had suffered from the disease. This is consistent with other studies where it was observed that young adults with an asymptomatic SARS-CoV-2 infection had memory B cell responses similar to those seen with a moderately symptomatic infection [ 38 ].…”

Section: Discussionsupporting

confidence: 92%

Seroprevalence of IgM/IgG and Neutralizing Antibodies against SARS-CoV-2 in Unvaccinated Young Adults from Mexico Who Reported Not Having Had a Previous COVID-19 Infection

Padilla-Bórquez,

Matuz-Flores,

Hernández-Bello

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). It is estimated that more than half of new infections are transmitted by asymptomatic people; therefore, the isolation of symptomatic people is not enough to control the spread of the disease. Methods. A total of 171 unvaccinated young adults (18–35 years) from Sonora, Mexico, who underwent a structured survey to identify prior COVID-19 infections, were included in this study. A qualitative determination of anti-SARS-CoV-2 antibodies in serum was performed by lateral flow immunoassay (Certum IgG/IgM Rapid Test™ cassette kit) and neutralizing antibodies were also determined (GenScript cPass assay). Results. A total of 36 people reported a history of COVID-19 infection, and 135 reported no history of COVID-19. In contrast, 49.6% (67/135) of individuals who had not reported a previous SARS-CoV-2 infection were seropositive to the rapid anti-SARS-CoV-2 antibody test, and 48.1% (65/135) of them had neutralizing antibodies. Conclusions. These results suggest that in young adults, SARS-CoV-2 infections could be asymptomatic in a high percentage of individuals, which could contribute in part to the slow control of the current pandemic due to the large number of asymptomatic cases that are contagious and that could be a silent spread of the virus.

show abstract

Section: Discussionsupporting

confidence: 92%

Seroprevalence of IgM/IgG and Neutralizing Antibodies against SARS-CoV-2 in Unvaccinated Young Adults from Mexico Who Reported Not Having Had a Previous COVID-19 Infection

Padilla-Bórquez,

Matuz-Flores,

Hernández-Bello

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

show abstract

“…The upregulation in interferon stimulated genes in asymptomatic COVID-19 patients was also found in other studies and was correlated with good clinical outcomes in these patients (51; 52). The upregulation in the formation of plasmablast and no change in essential genes for germinal center formation in asymptomatic patients from our study support the hypothesis that asymptomatic patients have a quick and effective innate immune response to control viral replication which helps to prevent a severe clinical outcome (53; 54).…”

Section: Discussionsupporting

confidence: 82%

Different B cell activation patterns in asymptomatic and symptomatic COVID-19 patients

Pham

Talih

Ehrhart³

et al. 2022

Preprint

View full text Add to dashboard Cite

Early and persistent defects in B cell subsets such as memory B cells were shown to be correlated with poor outcomes in COVID-19 patients. This research aimed to develop a molecular pathway model to understand the B cell development in COVID-19. A B cell transcriptomics dataset, obtained from COVID-19 patients, was analyzed on the resulting pathway model to study B cell activation. The pathway showed two distinct gene expression profiles between asymptomatic and symptomatic patients. In asymptomatic patients, there is an increase in transcript levels of antiviral interferon-stimulated genes such as ISG15, IFITM1, and NEAT1 and a driving gene for the extrafollicular pathway CXCR4 indicating a formation of plasmablast. In symptomatic patients, the results suggest an inhibition occurring at the germinal center hinting at a reduction in memory B cell production. Transcripts of driver gene CXCR5 involved in germinal center development is one of the most downregulated genes. This could contribute to the shortage in the formation of memory B cells in COVID-19. Concluding, in SARS-CoV-2 infection, B cells follow different activation routes in asymptomatic and symptomatic patients. In this study, we constructed a pathway that allowed us to analyze and interpret activation patterns of B cells in COVID-19 patients and their link to disease severity. Importantly, the pathway and approach can be reused for further research in COVID-19 or other diseases.

show abstract

“…ELISA was performed as previously described (38). Briefly, 1×phosphate buffered saline (1×PBS) was used as Coating Buffer; 1×PBS with 0.1% Tween 20 (1×PBST) was used as Wash Buffer; 5% Difco Skim Milk in 1×PBST was used as Blocking Buffer and Sample Dilution Buffer; IMMULON 4HBX 96-well, flat-bottom microplates (Thermo; Cat: 3855) were used as assay plates.…”

Section: Quantitative Elisa For Igg Subclassesmentioning

confidence: 99%

Antibodies to S2 domain of SARS-CoV-2 spike protein in Moderna mRNA vaccinated subjects sustain antibody-dependent NK cell-mediated cell cytotoxicity against Omicron BA.1

Balinsky,

Jiang,

Jani

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Vaccination with the primary two-dose series of SARS-CoV-2 mRNA protects against infection with the ancestral strain, and limits the presentation of severe disease after re-infection by multiple variants of concern (VOC), including Omicron, despite the lack of a strong neutralizing response to these variants. We compared antibody responses in serum samples collected from mRNA-1273 (Moderna) vaccinated subjects to identify mechanisms of immune escape and cross-protection. Using pseudovirus constructs containing domain-specific amino acid changes representative of Omicron BA.1, combined with domain competition and RBD-antibody depletion, we showed that RBD antibodies were primarily responsible for virus neutralization and variant escape. Antibodies to NTD played a less significant role in antibody neutralization but acted along with RBD to enhance neutralization. S2 of Omicron BA.1 had no impact on neutralization escape, suggesting it is a less critical domain for antibody neutralization; however, it was as capable as S1 at eliciting IgG3 responses and NK-cell mediated, antibody-dependent cell cytotoxicity (ADCC). Antibody neutralization and ADCC activities to RBD, NTD, and S1 were all prone to BA.1 escape. In contrast, ADCC activities to S2 resisted BA.1 escape. In conclusion, S2 antibodies showed potent ADCC function and resisted Omicron BA.1 escape, suggesting that S2 contributes to cross-protection against Omicron BA.1. In line with its conserved nature, S2 may hold promise as a vaccine target against future variants of SARS-CoV-2.

show abstract

Memory B-Cell Development After Asymptomatic or Mild Symptomatic SARS-CoV-2 Infection

Cited by 7 publications

References 14 publications

Seroprevalence of IgM/IgG and Neutralizing Antibodies against SARS-CoV-2 in Unvaccinated Young Adults from Mexico Who Reported Not Having Had a Previous COVID-19 Infection

Seroprevalence of IgM/IgG and Neutralizing Antibodies against SARS-CoV-2 in Unvaccinated Young Adults from Mexico Who Reported Not Having Had a Previous COVID-19 Infection

Different B cell activation patterns in asymptomatic and symptomatic COVID-19 patients

Antibodies to S2 domain of SARS-CoV-2 spike protein in Moderna mRNA vaccinated subjects sustain antibody-dependent NK cell-mediated cell cytotoxicity against Omicron BA.1

Contact Info

Product

Resources

About