Memory T cell subsets in healthy gingiva and periodontitis tissues

Mahanonda, Rangsini; Champaiboon, Chantrakorn; Subbalekha, Keskanya; Sa‐Ard‐Iam, Noppadol; Yongyuth, Arsarn; Isaraphithakkul, Benjarat; Rerkyen, Pimprapa; Charatkulangkun, Orawan; Pichyangkul, Sathit

doi:10.1002/jper.17-0674

Cited by 35 publications

(37 citation statements)

References 48 publications

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“…The extension of CD69 expression seems to be a function of bacteria and antigens exposure. In agreement with findings by Mahanonda et al on periodontitis tissue specimens harvested from sites of extracted teeth, the present study also showed a higher number of CD69+ T cells in the epithelium and connective tissue collected during flap operation procedures . A positive correlation was also observed between number of CD69+ and CD103+ T cells and PD, but the immunohistochemical analysis failed to demonstrate a gradient of these cells from the gingival margin to the bottom of the periodontal pockets.…”

Section: Discussionsupporting

confidence: 92%

“…Flow cytometry could be used to evaluate antigens co‐expression, but requires tissue fragmentation, thus losing information regarding cell localization (eg, intraepithelial or not). In this context, the study by Mahanonda et al demonstrated the presence of Trm cells in single cells suspension from periodontitis tissues of four patients but it did not distinguish between the epithelial or stromal localization . Novel approaches are now enabling precise spatial profiling of multiple antigens and could thus enable full characterization of these cells in future studies …”

Section: Discussionmentioning

confidence: 99%

“…Exclusion criteria for both groups were as follows: age younger than 18 years, systemic and immunologic diseases, or usage of medications that could interfere with periodontal status or the healing process, current smoking, pregnancy, lactation, a full‐mouth plaque score, and a full‐mouth bleeding score >20% before the surgical procedure, previous periodontal surgery at the affected teeth. Subjects who had taken anti‐inflammatory or antimicrobial drugs within the previous 3 months were also excluded . All enrolled patients a week before surgery underwent a session of professional oral hygiene.…”

Section: Methodsmentioning

confidence: 99%

“…In healthy gingiva, most of them were recirculating memory T cells and did not express CD69, while in periodontitis sites resident CD69+ memory T cells were prevalent. In both conditions, only a minority of memory T cells expressed CD103 . This finding is of particular interest because in treated periodontitis patients Trm cells could orchestrate an accelerated and enhanced host response to microbial challenge, leading to recurrence of periodontal disease.…”

Section: Introductionmentioning

confidence: 94%

“…In the literature, only a study investigated the presence of Trm cells in gingival tissues in healthy and periodontitis patients . In healthy gingiva, most of them were recirculating memory T cells and did not express CD69, while in periodontitis sites resident CD69+ memory T cells were prevalent.…”

Section: Introductionmentioning

confidence: 99%

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Resident memory T cells: Possible players in periodontal disease recurrence

Deli

Romano

Gualini

et al. 2019

J of Periodontal Research

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Background/objectives Tissue‐resident memory T cells (Trm) represent a new subset of long‐lived memory T cells that remain in barrier tissues after previous bacterial or viral infection to support early/immediate defense mechanisms, providing site‐specific protection from pathogen challenge. As data on Trm cells in human gingiva are just emerging, the aim of the present study was to explore their presence and distribution in epithelial and connective periodontal tissues in relation to microbial exposure and periodontal damage. Material and methods Periodontitis tissue specimens were collected from 20 generalized chronic periodontitis patients at the time of osseous resective surgery. As a control, 18 healthy tissue specimens were harvested each from both the primary flap and the palatal graft in 18 periodontally healthy patients during mucogingival surgeries. As CD69 and CD103 are phenotypic markers associated with tissue residence, intraepithelial and stromal CD103+ and CD69+ cells per high‐power field were counted in areas with highest expression. Double immunohistochemistry for CD3 and CD69 was performed to identify T cells. Results CD69 +and CD103+ cells showed a lymphocytic morphology, and double CD69 and CD3 staining confirmed the T cell phenotype of these cells. CD103 and CD69 expression was significantly enhanced in epithelial and connective tissues from patients with periodontitis compared with healthy controls (P < .001). Significant positive correlation between PD and both CD103 and CD69 epithelial expression was observed in tissue specimens from periodontitis patients (P < .001). Conclusion Within the limits of the present study, these results indicate that Trm cells are higher in periodontitis lesions. They could orchestrate the host response to microbial challenge, leading to a faster reactivation of periodontal disease.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Resident memory T cells: Possible players in periodontal disease recurrence

Deli

Romano

Gualini

et al. 2019

J of Periodontal Research

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Immunologic Diseases

Theofilou¹,

Konkel²,

Nikitakis³

et al. 2021

Burket's Oral Medicine

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Overexpression of PD‐L1 in gingival basal keratinocytes reduces periodontal inflammation in a ligature‐induced periodontitis model

Wongtim

Ikeda

Ohno

et al. 2021

Journal of Periodontology

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Background:The immune checkpoint programmed cell death 1 (PD-1): PD-1 ligand 1 (PD-L1) pathway plays a crucial role in maintaining immune tolerance and preventing tissue damages by excessive immune responses. PD-L1 is physiologically expressed and upregulated in keratinocytes (KCs) in the oral cavity. We here investigated the contribution of PD-L1 that was overexpressed in gingival basal KCs in a ligature-induced periodontitis model. Methods: Wild-type (WT) BALB/c and K14/PD-L1 transgenic (tg) mice, in which PD-L1 was overexpressed in basal KCs under control of the keratin 14 promoter, were used. To induce periodontitis, a 9-0 silk ligature was placed around the upper right second molar, and lipopolysaccharide from Porphyromonas gingivalis was applied on the suture. Gingival tissues were collected on day 7, after which histological analyses were performed, including by hematoxylin and eosin and tartrate-resistant acid phosphate staining (TRAP) and quantitative PCR for proinflammatory cytokines and bone metabolism-related genes. Alveolar bone loss at 7 weeks after ligature placement was assessed by micro-computed tomography analysis. Results: PD-L1 was overexpressed in the basal KCs of all gingival epithelia in K14/PD-L1tg mice. Early ligature-induced periodontal inflammation, as assessed based on histological changes, elevation of proinflammatory cytokine (IL-1β, IL-6, TNF-α) expression, periodontal ligament degeneration, and osteoclastogenesis as assessed by Rankl and Opg expression and TRAP+ cells, was markedly impaired in K14/PD-L1tg mice. Alveolar bone resorption at a late time point was also clearly minimized in K14/PD-L1tg mice. Conclusion: Overexpression of PD-L1 in gingival basal keratinocytes in K14/PD-L1tg mice reduces periodontal inflammation and alveolar bone resorption in a ligature-induced periodontitis model.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Memory T cell subsets in healthy gingiva and periodontitis tissues

Cited by 35 publications

References 48 publications

Resident memory T cells: Possible players in periodontal disease recurrence

Resident memory T cells: Possible players in periodontal disease recurrence

Immunologic Diseases

Overexpression of PD‐L1 in gingival basal keratinocytes reduces periodontal inflammation in a ligature‐induced periodontitis model

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