2008
DOI: 10.1007/s10544-007-9153-4
|View full text |Cite
|
Sign up to set email alerts
|

MEMS-based fabrication and microfluidic analysis of three-dimensional perfusion systems

Abstract: This paper describes fabrication and fluidic characterization of 3D microperfusion systems that could extend the viability of high-density 3D cultures in vitro. High-aspect ratio towers serve as 3D scaffolds to support the cultures and contain injection sites for interstitial delivery of nutrients, drugs, and other reagents. Hollow and solid-top tower arrays with laser ablated side-ports were fabricated using SU-8. Appropriate sizing of fluidic ports improves the control of agent delivery. Microfluidic perfusi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
6
0

Year Published

2009
2009
2024
2024

Publication Types

Select...
6

Relationship

2
4

Authors

Journals

citations
Cited by 8 publications
(6 citation statements)
references
References 16 publications
0
6
0
Order By: Relevance
“…Here, we cured the 3-D neuronal-astrocytic co-culture constructs described previously (see Section III) around high aspect ratio tower arrays fabricated using SU-8. [180][181][182] Our objective was to study 3-D culture interactions and distribution (cell and matrix) at the tower interface, which in this case was a surrogate for fully functional MEMS-based hardware such as 3-D electrode arrays (Fig. 14).…”
Section: Vc Neural-electrode Interface In Vitromentioning
confidence: 99%
“…Here, we cured the 3-D neuronal-astrocytic co-culture constructs described previously (see Section III) around high aspect ratio tower arrays fabricated using SU-8. [180][181][182] Our objective was to study 3-D culture interactions and distribution (cell and matrix) at the tower interface, which in this case was a surrogate for fully functional MEMS-based hardware such as 3-D electrode arrays (Fig. 14).…”
Section: Vc Neural-electrode Interface In Vitromentioning
confidence: 99%
“…Fluid velocity was measured by imaging the displacement of 550 nm fluorescent tracers suspended in DI water using an episcopic, darkfield fluorescence arrangement (Choi et al 2008;Cullen et al 2007). Particles were sufficiently small to faithfully follow the flow without influencing it [particle Stokes number ≪ 0.14; (Dring 1982)].…”
Section: Flow and Thermal Diagnosticsmentioning
confidence: 99%
“…Velocity distributions were measured in planes that were parallel to the perfusion source by adjusting the distance between the perfusion chamber and the imaging optics. Sophisticated image processing and cross-correlation of particle images (Choi et al 2008;Cullen et al 2007) were used to extract velocity measurements at a resolution that was within 5% of the microjet injection velocity.…”
Section: Flow and Thermal Diagnosticsmentioning
confidence: 99%
“…A subset of the MEMS devices appeared in the latter part of the 1980s termed ''lab-on-a-chip''. A wide range of novel mechanical systems emerged utilising new MEMS influenced designs which utilise low volume liquid handling devices [38][39][40][41][42][43] and biochip platforms. 30,[44][45][46] There were a significant number of advantages in using these designs from the point of view of cost and time.…”
Section: Micro-electromechanical Systemsmentioning
confidence: 99%