Experimental flow visualization is a valuable tool for analyzing microfluidics and nanofluidics in a wide variety of applications. Since the late 1990s, considerable advances in optical methods and image postprocessing techniques have improved direct optical measurements, resulting in an accurate qualitative and quantitative understanding of transport phenomena in lab-on-a-chip capillaries. In this study, a comparison of different optical measurement techniques is presented. The state-of-the-art development of particle image velocimetry to date, particularly in microscale applications, is reviewed here in detail. This study reviews novel approaches for estimating velocity field measurements with high precision within interrogation windows. Different regularization terms are discussed to demonstrate their capability for particle displacement optimization. The discussion shows how single- and multi-camera optical techniques provide two-dimensional and three-component velocity fields. The performance of each method is compared by highlighting its advantages and limitations. Finally, the feasibility of micro resolution particle image velocimetry in bioapplications is overviewed.