Menstrual cycle–related sialidase activity of the female cervical mucus is associated with exosome-like vesicles

Flori, F.; Secciani, Federica; Capone, Antonietta; Paccagnini, Eugenio; Caruso, Sabrina; Ricci, M.; Focarelli, Riccardo

doi:10.1016/j.fertnstert.2007.01.209

Cited by 19 publications

(16 citation statements)

References 29 publications

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“…In general, changes in glycosidase activity generally matched tlie changes in the binding of the sugars that they degrade but may indicate a constant remodeling of the mucins after secretion and not regulation of their absolute concentrations. There are few publications reporting glycosidase activities in the reproductive tract but the I)resent data agree with a report that ß-galactosidase activity in uterine lumen of gilts was greatly increased l)y progesterone treatment (Hansen et al, 1985) and a report that the greatest sialidase activity in human cervical mucus occurs at ovulation (Flori et al, 2007).…”

Section: Discussionsupporting

confidence: 72%

Glycoproteins and glycosidases of the cervix during the periestrous period in cattle1

Pluta

Irwin

Dolphin

et al. 2011

Journal of Animal Science

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The cervix and its secretions undergo biochemical and physical changes under the differential influences of estrogen and progesterone. These include changes in the glycoprotein profile of the endocervix and its secretions. A comprehensive survey of such changes in cervical epithelium and cervical secretions was performed on bovine samples throughout the periestrous period. Cervical tissue samples and swabs were collected from synchronized beef heifers that were slaughtered 1) 12 h after controlled intravaginal progesterone-releasing device (CIDR) removal, 2) 24 h after CIDR removal, 3) at the onset of estrus, 4) 12 h after the onset of estrus, 5) 48 h after the onset of estrus, and 6) 7 d after the onset of estrus. Histological staining with hematoxylin and eosin, periodic acid Schiff, Alcian blue, and high-iron diamine was carried out to map overall patterns of stored glycoproteins and tissue structure. Biotinylated lectins were also used to detect the presence and distribution of a range of saccharide structures. The activities of β-galactosidase, α-L-fucosidase, β-N-acetyl-hexosaminidase, and sialidase were measured in cervical swabs using specific substrates. The epithelial layer of the cervix exhibited dynamic changes in cellular hypertrophy and amounts of stored glycoprotein. The greatest content of neutral and acidic mucins was observed 48 h after onset of estrus (P < 0.05). Sialylated mucins predominated at the bases of cervical folds, whereas sulfated mucins were more abundant (P < 0.05) at their apices. The stained area of core mucin glycans changed (P < 0.05) in association with follicular versus luteal phases, whereas terminal glycans changed (P < 0.05) mainly at the time of estrus and shortly thereafter. The greatest activity of β-galactosidase and sialidase was observed 12 h after onset of estrus, whereas β-hexosaminidase and α-fucosidase peaked at the luteal time point (P < 0.05). Taken together, we suggest that the well-known changes in the endocervix and its secretions that are associated with the physiological modulation of sperm transport and function of the cervical barrier are, in part, driven by glycosylation changes.

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Section: Discussionsupporting

confidence: 72%

Glycoproteins and glycosidases of the cervix during the periestrous period in cattle1

Pluta

Irwin

Dolphin

et al. 2011

Journal of Animal Science

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“…Notably, the subcellular localizations of Neu1-4 are reported to vary in response to physiological changes (15). Although Neu1-4 are generally recognized as intracellular enzymes, their activities have been detected in the extracellular space (20,21), a finding that supports to the notion of in situ modification of cell-surface sialylation. However, no robust functional or mechanistic studies have examined extracellular sialidases secreted from cells.…”

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confidence: 65%

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin

Sumida

Hane

Yabe

et al. 2015

Journal of Biological Chemistry

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Background:Although polySia is known to retain neurotrophins, their releasing mechanism remains unknown. Results: PolySia present on the cell surface of microglia is rapidly cleared by Neu1 sialidase on exovesicles secreted upon inflammatory stimulus, leading to neurotrophin release. Conclusion: Exovesicular Neu1 regulates rapid turnover of polySia and concomitant neurotrophin function. Significance: First demonstration of on-site turnover of polySia and its physiological significance.

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“…Sialylation and desialylation of other gonococci in the genital tract are dynamic processes; neuraminidases (sialidases) elaborated by vaginal microflora (54) and by cervical epithelia (59, 60) may result in desialylation of gonococci. Here we have shown that PorB.1A strains that are not sialylated and bind fH adhere more efficiently to CR3-expressing cells.…”

Section: Discussionmentioning

confidence: 99%

Factor H Facilitates Adherence of Neisseria gonorrhoeae to Complement Receptor 3 on Eukaryotic Cells

Agarwal

Ram

Ngampasutadol

et al. 2010

The Journal of Immunology

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Neisseria gonorrhoeae can engage human complement receptor 3 (CR3) directly or through surface-bound iC3b. Factor H (fH) that binds to bacteria facilitates conversion of C3b to iC3b. fH also binds directly to CR3 on professional phagocytes. Certain nonprofessional phagocytes, such as primary cervical epithelial cells, also express CR3. We hypothesized that fH could bridge bacteria to CR3 and facilitate gonococcal association with host cells. Specificity of the fH–CR3 interaction was confirmed using human CR3-transfected Chinese hamster ovary (CHO-CR3) cells. Using recombinant proteins that comprised contiguous fH domains (fH contains 20 short consensus repeat [SCR] domains) fused to murine Fc, we observed strong binding through SCRs 18–20, whereas weaker binding occurred through SCRs 6–10. Both regions also bound to unsialylated porin (Por) B.1A-expressing N. gonorrhoeae. Accordingly, fH-related protein 1 (three of its five SCRs are highly homologous to fH SCRs 18–20) bound to CHO-CR3 and to unsialylated PorB.1A gonococci. An alternatively spliced variant of fH called fH-like protein-1 (contains fH SCRs 1–7) bound to gonococci but minimally to CHO-CR3. An fH SCRs 6–20 construct enhanced binding of unsialylated PorB.1A gonococci to CHO-CR3. However, a construct that contained only the apparently relevant SCRs (6, 7, and 18–20) bound to CHO-CR3 and to gonococci separately, but did not enhance bacteria–CR3 interactions, suggesting that the intervening SCRs (8–17) may impart a configurational and spatial requirement for fH to bridge gonococci to CR3. These results indicate adherence between fH-coated gonococci and CR3 and may provide a means for gonococci to gain sanctuary into nonprofessional phagocytes.

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Menstrual cycle–related sialidase activity of the female cervical mucus is associated with exosome-like vesicles

Cited by 19 publications

References 29 publications

Glycoproteins and glycosidases of the cervix during the periestrous period in cattle1

Glycoproteins and glycosidases of the cervix during the periestrous period in cattle1

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin

Factor H Facilitates Adherence of Neisseria gonorrhoeae to Complement Receptor 3 on Eukaryotic Cells

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