Background: Whiles challenges associated with menstrual hygiene management among adolescents are universal, some sub-populations, such as deaf girls, have peculiar challenges that requires special attention. Although there have been numerous studies on menstrual hygiene management among adolescents in the school setting in Ghana, the experiences of students with disabilities, particularly, deaf girls have not attracted adequate scholarly attention.Objective: The objective of the study was to generate new empirical evidence on menstrual hygiene management challenges of deaf adolescent girls in the special schools for the deaf in Ghana. The overall objective was to obtain data that could be used to improve menstrual hygiene practices among the target population, and, to bridge the gap in the existing literature regarding menstrual hygiene management experiences of in-school adolescent deaf girls in Ghana.Method: This study adopted a mixed method approach to investigate the menstrual hygiene management challenges of in-school deaf adolescent girls in Ghana. A total of 152 participants comprising 118 schoolgirls, 15 boys, seven school heads, six teachers, and six school housemothers from seven special schools were involved in the study.Results: The findings identified five challenges facing the girls. These are inadequate access to sanitary pads, inappropriate school WASH facilities, health related problems, negative attitude of male colleagues, and academic related challenges.Conclusion: The findings indicate that the quality of WASH facilities in the schools require improvement. We recommend that access to sanitary kits for underprivileged girls should be made a priority issue, boys and male teachers should be included in all menstrual hygiene management awareness programs, whiles the capacity of school housemothers on menstrual hygiene management should be enhanced. The study made important contributions to the existing literature on disability and menstrual health in Africa.