Background: Patient rights have become an essential part of novel healthcare practice. High quality as well as safe healthcare services have frequently been assessed and examined for the conditions of patient rights adherence by the healthcare team. However, mentally ill personnel are revealed to a broad range violation of human rights. Aim: This research aimed to assess nursing staff knowledge and compliance regarding psychiatric patients' rights and its relationship with family caregivers' awareness. Design: A descriptive correlative design was carried out to achieve the aim. Setting: the study conducted at Psychiatric Health and Addiction Hospital in Minia Governorate, Egypt. Subjects: A convenience sample of all staff nurses (n=66) and a representative sample from family caregivers (n=100). Data Collection Tools; An Observation Checklist and Nurses Knowledge as well as Family Caregivers Awareness Questionnaires. Results: Most of the study participants have satisfactory knowledge level while many of them practice poor compliance, and more than half of family caregivers had moderate level of awareness regarding psychiatric patient rights. Additionally, strong positive correlation exists among nurse's knowledge, and compliance regarding psychiatric patients' rights as well as family caregivers' awareness. Conclusion: Health care providers' knowledge and practices are reflected on the awareness of family caregivers about patient rights and care activities. Recommendations: Ongoing assessment compliance of nurses toward patients' rights also training programs and workshops need to be carried out for both nurses and family caregivers.