Mentha piperita L. Micropropagation and the Potential Influence of Plant Growth Regulators on Volatile Organic Compound Composition

Abstract

Due to the industrial use of Mentha piperita L. (peppermint), it is important to develop an optimal method to obtain standardized plant material with specific quality parameters. In vitro cultures may allow the production of desirable odor-active compounds (OACs) and improve their share in the plant aroma profile. There are two types of explants that are commonly used, apical meristems and nodal segments. In this study, the best overall effects were shown to be produced by the combination of MS medium with the addition of 0.5 mg·dm−3 indolyl-3-butyric acid. In this case, a very high degree of rooting was found (97% for apical meristems, 100% for nodal meristems), lateral shoots were induced in 83% of both types of explant, and the content of OACs in the plant aroma profile increased significantly, especially menthofurolactone and cis-carvone oxide, responsible in this case for a characteristic mint-like aroma. The comparison of the volatile organic compounds (VOCs) obtained from plants of different origin by GC-MS showed no significant differences in their qualitative composition. Moreover, in-vitro-cultivated peppermint on a medium containing 0.5 mg·dm−3 2-isopentinloadenine and 0.1 mg·dm−3 indolyl-3-acetic acid showed significant amounts of menthofurolactone in its VOC composition.

Transcriptome Analysis Reveals Upregulated Secondary Metabolite Pathways in Micropropagated Lawsonia Inermis L.

“…Tissue culture also provides various avenues wherein the plant's metabolic pathways can be manipulated, resulting in in vitro plants with increased secondary metabolic content. Previous studies have shown similar results in Mentha, which when grown in MS media, supplemented with indole-butyric acid (Lyczko et al 2020) showed higher accumulation of odor active and volatile compounds.…”

Sarracenia alata (Alph.Wood) Alph.Wood Microcuttings as a Source of Volatiles Potentially Responsible for Insects’ Respond

“…The major representatives (more than 4.00%) were pyridine (3.10% ± 0.07), ( Z )-3-hexen-1-ol (16.48% ± 0.31), ( E )-3-hexen-1-ol acetate (19.99% ± 0.01), p -cymene (4.96% ± 0.02), ( E )-α-bergamotene (4.15% ± 0.19), β-caryophyllene (11.30% ± 0.27) and labda-8(20),13(16),14-triene (4.89% ± 0.09)., which may be found in other, various species like Lavandula angustifolia Mill. [ 24 ], Coriandrum sativum L. [ 25 ], Mentha piperita L. [ 26 ], Cannabis sativa L. [ 27 ] and Boletus edulis [ 28 ]. Especially interesting was high content of ( E )-α-bergamotene, which is characteristic for Citrus bergamia Risso fruit [ 29 ] and pyridine, which was reported in similar amounts in Cicer arietinum L. [ 30 ].…”

Chemical Components and Biological Activities of Essential Oils of Mentha × piperita L. from Field-Grown and Field-Acclimated after In Vitro Propagation Plants

“…[16,[20][21][22][23][24]. The use of growth regulators increases the production of EOs by peppermint plants and affects the chemical composition of EOs [18,22,[25][26][27]. The amount of the individual components contained in the EOs changes and undesirable substances are not synthesized, which was confirmed by the experiment of Bertoli et al [20].…”

“…The cv Tik-Tak had the maximum multiplication factor and cv Serebristaya had the minimum. Full MS medium supplemented with 1.0 mg•L -1 IBA is optimal for in vitro root induction in mint [17,22,40]. A number of studies have found that mint shoots can form roots spontaneously [41,42].…”

“…The results obtained indicate that the studied genotypes have a specific response to specific conditions in the plant tissue culture. [16,[20][21][22][23][24]. The use of growth regulators increases the production of EOs by peppermint plants and affects the chemical composition of EOs [18,22,[25][26][27].…”