2009
DOI: 10.1073/pnas.0807899106
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MENε/β noncoding RNAs are essential for structural integrity of nuclear paraspeckles

Abstract: Recent transcriptome analyses have shown that thousands of noncoding RNAs (ncRNAs) are transcribed from mammalian genomes. Although the number of functionally annotated ncRNAs is still limited, they are known to be frequently retained in the nucleus, where they coordinate regulatory networks of gene expression. Some subnuclear organelles or nuclear bodies include RNA species whose identity and structural roles are largely unknown. We identified 2 abundant overlapping ncRNAs, MEN and MEN␤ (MEN/␤), which are tra… Show more

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Cited by 556 publications
(649 citation statements)
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“…Importantly, no floating cells (dead cells) were seen by depletion of NR_015389, AK091718, and AK055657 under microscopic observation (data not shown), suggesting that these SLiTs are modifying factors but are not essential for cell proliferation. Biochemical fractionation indicated that NR_015389, AK091718, AK055657, AK123363, and NR_033895 were predominantly enriched in the heavy fractions of the sucrose density-gradient analysis, along with nuclear lncRNAs, such as NEAT1 (Clemson et al 2009;Sasaki et al 2009;Sunwoo et al 2009) and MALAT1, which are components of nuclear bodies ( Fig. 5B; Supplemental Fig.…”
Section: Bric-seq Reveals Hundreds Of Slits In Mammalsmentioning
confidence: 99%
“…Importantly, no floating cells (dead cells) were seen by depletion of NR_015389, AK091718, and AK055657 under microscopic observation (data not shown), suggesting that these SLiTs are modifying factors but are not essential for cell proliferation. Biochemical fractionation indicated that NR_015389, AK091718, AK055657, AK123363, and NR_033895 were predominantly enriched in the heavy fractions of the sucrose density-gradient analysis, along with nuclear lncRNAs, such as NEAT1 (Clemson et al 2009;Sasaki et al 2009;Sunwoo et al 2009) and MALAT1, which are components of nuclear bodies ( Fig. 5B; Supplemental Fig.…”
Section: Bric-seq Reveals Hundreds Of Slits In Mammalsmentioning
confidence: 99%
“…In addition to the structural function of the RNA within the cell cytoplasm, recent studies of the chromatin and chromosome structure, nuclear architecture, nuclear bodies, and the mechanisms of DNA replication indicate the presence of structural RNAs in the cell nucleus (Mattick et al 2009;Sasaki et al, 2009). Recently, RNA was found to play a structural role in the organization of chromatin from chicken liver and Drosophila S2 cell nuclei (Rodrıguez-Campos and Azorın, 2007).…”
Section: Structural Role Of Rna In Cell Nucleusmentioning
confidence: 99%
“…Recent large-scale analyses of transcripts present in mammalian cell nucleus have found thousands of long noncoding RNAs (ncRNAs), which may be involved in the organization of the genome (Amaral et al, 2008). Sasaki et al (2009) identified two of these ncRNAs, MENe and MENb (MENe/b), as a structural component of the nuclear paraspeckles, the enigmatic nuclear bodies possibly involved in the regulation of gene-expression through the nuclear retention of RNA. These authors show that the knockdown removal of MENe/b attenuate structural integrity of the paraspeckles causing their disintegration (Sasaki et al, 2009).…”
Section: Structural Role Of Rna In Cell Nucleusmentioning
confidence: 99%
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