Mercuric reductase activity of multiple heavy metal‐resistant Lysinibacillus sphaericus G1

Bafana, Amit; Chakrabarti, Tapan; Krishnamurthi, Kannan

doi:10.1002/jobm.201300308

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…Similarly, two Lysinibacillus strains (L. xylanilyticus and L. marcoides) showed promising results in reducing toxic Se oxyanions into elemental Se nanoparticles [52]. In addition, Bafana et al [53] reported that L. sphaericus can detoxify Cr and Hg by reducing them. The second mechanism is biosorption, which is the binding of metal ions with metal-binding proteins present on the bacterial cell wall.…”

Section: Potential As a Metal Bioremediation Agentmentioning

confidence: 99%

Lysinibacillus Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents

Nusrat

Shimizu

2021

RAS

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The use of synthetic chemicals has increased drastically due to industrialization and urbanization. However, the long-term and indiscriminate use of these chemicals has a negative impact on environment and human health; thus, public concerns about the hazardous effects of such synthetic chemicals are increasing day by day. To solve these problems, the exploitation of potential alternatives has become a major challenge, and the admiration of beneficial microbes is increasing due to their safe and environment-friendly nature. Microbes can mitigate the hazardous effects of synthetic chemicals by reducing their use and toxicity. Lysinibacillus species are gram-positive, spore-forming, motile bacteria. This genus was previously designated as Bacillus spp. under the family Bacillaceae of the phylum Firmicutes. For a long period of time, Lysinibacillus is well-known for its insecticidal activity against various insects, including mosquitoes, which are the vector of several human diseases. In addition, some Lysinibacillus species have a potential for heavy metal remediation. In recent years, Lysinibacillus spp. are attracting the researchers' attention as plant growth-promoting and disease control agents, which would be used as alternatives to agrochemicals. This study gives an overview of the entomopathogenic, bioremediation, plant growth-promoting, and biological disease control abilities of the genus Lysinibacillus.

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Section: Potential As a Metal Bioremediation Agentmentioning

confidence: 99%

Lysinibacillus Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents

Nusrat

Shimizu

2021

RAS

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“…Although the reduction pathway was not studied here, it is suggested that a rapid reduction of Au(III) to Au(I) occurs in the cell wall and is followed by a slow reduction to Au(0) [30]. This reduction pathway could compromise the S-layer [31] and a mer-like cluster, the latter of which confers microbial resistance to mercury by reducing the toxic metal to Hg(0) with the reductase enzyme, MerA [32]. This enzyme could also work on gold species due to the similarities between the metals [30].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Gold Biosorption of Lysinibacillus sphaericus CBAM5 by Encapsulation of Bacteria in an Alginate Matrix

Páez-Vélez

Rivas

Dussán

2019

Metals

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Given its variety of properties, including conductivity and slow corrosion, the industrial uses for gold are increasing dramatically. This means that greater amounts of gold are being released into the environment and that a biological approach to recycling gold is of great interest. Lysinibacillus sphaericus, a bacterium capable of metal accumulation inside the cell and adsorption in the external surface, was encapsulated in an alginate matrix to improve the capture of gold from aqueous media. In this study, L. sphaericus CBAM5 proved to have the greatest potential compared to other strains and, following its encapsulation, the efficiency for the removal of the precious metal, at a concentration of 60 ppm, was 100% after three hours of exposure. It was identified that the alginate spheres with bacteria could also be reused. In fact, an efficiency of 60% was retained after three cycles of utilization. Thus, alginate acts as an adequate immobilization matrix for bacteria as a highly effective gold capture mechanism, which also shows great potential as an alternative for biotechnological applications.

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“…These bacterial model strains were selected to assess the differential influence of NPs on antimicrobial activity considering their distinct cell wall architectures. Both bacterial strains are known for their resistance to environmental stress, such as heavy metal or antibiotic exposure, − which make them suitable models for studying bacterial drug resistance. Notably, S.…”

Section: Methodsmentioning

confidence: 99%

Exploring Antibacterial Activity and Bacterial-Mediated Allotropic Transition of Differentially Coated Selenium Nanoparticles

Ruiz-Fresneda

Schaefer

Hübner

et al. 2023

ACS Appl. Mater. Interfaces

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The use of metal nanoparticles (NPs) as antimicrobial agents has become a promising alternative to the problem of antibiotic-resistant bacteria and other applications. Silver nanoparticles (AgNPs) are well-known as one of the most universal biocide compounds. However, selenium nanoparticles (SeNPs) recently gained more attention as effective antimicrobial agents. This study aims to investigate the antibacterial activity of SeNPs with different surface coatings (BSA-coated, chitosan-coated, and undefined coating) on the Gram-negative Stenotrophomonas bentonitica and the Gram-positive Lysinibacillus sphaericus in comparison to AgNPs. The tested NPs had similar properties, including shape (spheres), structure (amorphous), and size (50−90 nm), but differed in their surface charge. Chitosan SeNPs exhibited a positive surface charge, while the remaining NPs assayed had a negative surface charge. We have found that cell growth and viability of both bacteria were negatively affected in the presence of the NPs, as indicated by microcalorimetry and flow cytometry. Specifically, undefined coating SeNPs displayed the highest percentage values of dead cells for both bacteria (85−91%). An increase in reactive oxygen species (ROS) production was also detected. Chitosan-coated and undefined SeNPs caused the highest amount of ROS (299.7 and 289% over untreated controls) for S. bentonitica and L. sphaericus, respectively. Based on DNA degradation levels, undefined-SeNPs were found to be the most hazardous, causing nearly 80% DNA degradation. Finally, electron microscopy revealed the ability of the cells to transform the different SeNP types (amorphous) to crystalline SeNPs (trigonal/monoclinical Se), which could have environmentally positive implications for bioremediation purposes and provide a novel green method for the formation of crystalline SeNPs. The results obtained herein demonstrate the promising potential of SeNPs for their use in medicine as antimicrobial agents, and we propose S. bentonitica and L. sphaericus as candidates for new bioremediation strategies and NP synthesis with potential applications in many fields.

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Mercuric reductase activity of multiple heavy metal‐resistant Lysinibacillus sphaericus G1

Cited by 12 publications

References 21 publications

<i>Lysinibacillus</i> Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents

<i>Lysinibacillus</i> Species: Their Potential as Effective Bioremediation, Biostimulant, and Biocontrol Agents

Enhanced Gold Biosorption of Lysinibacillus sphaericus CBAM5 by Encapsulation of Bacteria in an Alginate Matrix

Exploring Antibacterial Activity and Bacterial-Mediated Allotropic Transition of Differentially Coated Selenium Nanoparticles

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