Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision

Chang, Yung Hsien; Lee, Wei-Yuan; Lin, Yu‐Chih; Hsu, Todd

doi:10.1016/j.aquatox.2017.09.001

Cited by 17 publications

(7 citation statements)

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“…Some are indirect. For example, Hg 2+ ions promote the formation of reactive oxygen species (ROS) [40][41][42] and can also disrupt DNA repair processes [43,44]. On the other hand, other mechanisms result from the direct interaction with DNA.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Alkylation of uracil and thymine in the gas phase through interaction with alkylmercury compounds

Salpin

Haldys

Latrous

et al. 2019

International Journal of Mass Spectrometry

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Section: Accepted Manuscriptmentioning

confidence: 99%

Alkylation of uracil and thymine in the gas phase through interaction with alkylmercury compounds

Salpin

Haldys

Latrous

et al. 2019

International Journal of Mass Spectrometry

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“…Високий показник біоакумуляції, особливо для водних екосистем, робить цей метал надзвичайно небезпечним та опосередковує її надзвичайно високу токсичність (Eagles-Smith et al, 2016b;Orihel et al, 2007). Визначені численні клітинні пошкодження, що супроводжують біоакумуляцію ртуті у риб (Carocci et al, 2014;Chang et al, 2017), яка накопичується у зябрах, нирках, печінці, м'язах та мозку (Simon, Boudou, 2001;Amlund et al, 2007;Vieira et al, 2009;Brandão et al, 2015;Gómez -Оlivan et al, 2017).…”

Section: вступunclassified

Indices of the DNA repair system in the brain of fish as a biomarker of inorganic mercury burden

Nedzvetsky

Gasso

Novitskyi

et al. 2021

ean

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Mercury is a widespread heavy metal that causes a stable and prolonged environmental pollution. Low concentrations of inorganic and organic mercury compounds are found in almost all water bodies. The high level of mercury bioaccumulation is a cause of tissue-specific toxicity, including neurotoxicity. Absorbed in nervous tissue mercury can cause brain disorders both in neural and glial cells. The brain of fish is considered one of the most susceptible targets for cytotoxicity of mercury in aquatic ecosystems. Taking into account that different forms of mercury have widespread distribution and exhibit a strong neurotoxic effect, the assessment of mercury cytotoxicity in the brain of fish is relevant and extremely important. Rainbow trout Oncorhynchus mykiss was exposed to mercury chloride in the dose range of 5-20 μg/L for 60 days to study the chronic exposure of low doses. In this paper, we studied the influence of inorganic mercury on oxidative stress, DNA repair proteins – ERCC1 and PARP1 in the trout’s brain. The results obtained have shown that the chronic effect of inorganic mercury causes dose-dependent oxidative stress in the fish brain. In addition, low concentrations of mercury (10 and 20 μg/L) caused a decrease in the content of ERCC1 in the brain of fish. On the contrary, the same doses have caused an increase in PARP1 expression. That is the chronic influence of low concentrations of inorganic mercury has a negative effect in the fish brain. Observed results showed that inorganic mercury has a potential for suppressing DNA repair and, therefore, increases the instability of genome. Thus, ERCC1 and PARP1 can be considered as the sensitive biomarkers of mercury cytotoxicity in the fish brain. A further study of mercury neurotoxicity is needed to find out the hazard of mercury environmental pollution as well as a validation of biomarkers of their impact.

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“…The most meaningful bioaccumulation of Hg is therefore considered in aquatic organisms in compare to other heavy metals (Orihel et al, 2007;Eagles-Smith et al, 2016a). Hg can induce multifaceted cellular damages in the fish tissues as well as mammals (Carocci et al, 2014;Chang et al, 2017). The bioaccumulation of Hg species was determined in several fish types, especially Cyprinus carpio, Salmo salar, Pomatoschistus microps, Liza aurata in the gills, kidneys, liver, muscle and brain (Simon andBoudou, 2001: Amlund et al, 2007;Vieira et al, 2009;Brandão et al, 2015;Gómez-Oliván et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

Taysi¹,

Söğüt²,

Nedzvetsky³

et al. 2019

Türk Tarım Ve Doğa Bilimleri Dergisi

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Mercury (Hg) is one of most toxic and widespread element of aquatic environment. Almost every kind of the fish can accumulate Hg. Hg-induced peculiarities of cellular malfunction could be used as adequate biomarker to estimate the contamination risk in polluted aquatic ecosystems. The brain cells are high susceptible to the Hg compounds cytotoxicity. Various Hg species have different harmful effects on both structure and function of the brain cells. Neurotoxicity of inorganic Hg remains discussable and studied restrictedly. In this study, we have studied the role of RPA1 and p53 proteins in brain cell response to sublehtal (25% LD 50 and 50% LD 50) doses of inorganic Hg in rainbow trout (Oncorhynchus mykiss). LD 50 value of Hg chloride in presented study was determined as 551 µg/L relate to 96 hours exposure. Two sublethal doses were used in the exposure rainbow trout at 2 and 7 days. The treatment with Hg chloride induced in fish brain dose-dependent increase in ROS level as well as time-dependent growth. Moreover, the exposure to both 25% and 50% LD 50 Hg doses have caused significant upregulation of RPA1 expression. In the brain tissue of fish exposed to Hg for 2 days, it stimulated slightly expression of p53. Contrary, 7 days exposure induced significant decrease in p53 expression. The results of presented study evidence that sublethal doses of inorganic Hg are extremely neurotoxic and can induce in the fish brain signaling pathways disturbance through decline of stress sensor protein p53. Besides, the increase in RPA1 expression let to assume that brain cells of the fish can repair ROS-induced DNA breaks and prevent genotoxic effect of inorganic Hg. Overall, current data pointed out that inorganic mercury is high toxic to fish brain cells and this question requires future research.

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Mercury (II) impairs nucleotide excision repair (NER) in zebrafish (Danio rerio) embryos by targeting primarily at the stage of DNA incision

Cited by 17 publications

References 50 publications

Alkylation of uracil and thymine in the gas phase through interaction with alkylmercury compounds

Alkylation of uracil and thymine in the gas phase through interaction with alkylmercury compounds

Indices of the DNA repair system in the brain of fish as a biomarker of inorganic mercury burden

Sublethal Doses of Inorganic Mercury Induce Dose-Depended Upregulation of RPA1 Content and Inhibit p53 Expression in the Brain of Rainbow Trout (Oncorhynchus mykiss)

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