Mercury (II) sensor based on monitoring dissociation rate of the trans-acting factor MerR from cis-element by surface plasmon resonance

Taniguchi, Mitsugu; Siddiki, Mohammad Shohel Rana; Ueda, Shunsaku; Maeda, Isamu

doi:10.1016/j.bios.2014.08.040

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…A mercury (II) sensor, based on the dissociation rate of the trans-acting factor MerR from the cis-element, was investigated by SPR [128]. The sensor, modified with dsDNA including the cis-element (Pmer), can monitor the dissociation stage of MerR or protein-tagged MerR from the cis-element, enabling measurement of Hg 2+ with a LOD of 5 µg/L.…”

Section: Biosensing Methodsmentioning

confidence: 99%

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

et al. 2019

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: Microsystems and biomolecules integration as well multiplexing determinations are key aspects of sensing devices in the field of heavy metal contamination monitoring. The present review collects the most relevant information about optical biosensors development in the last decade. Focus is put on analytical characteristics and applications that are dependent on: (i) Signal transduction method (luminescence, colorimetry, evanescent wave (EW), surface-enhanced Raman spectroscopy (SERS), Förster resonance energy transfer (FRET), surface plasmon resonance (SPR)); (ii) biorecognition molecules employed (proteins, nucleic acids, aptamers, and enzymes). The biosensing systems applied (or applicable) to water and milk samples will be considered for a comparative analysis, with an emphasis on water as the primary source of possible contamination along the food chain.

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Section: Biosensing Methodsmentioning

confidence: 99%

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

et al. 2019

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“…Poly(ethylene) glycol (PEG) is one of the most studied polymers as stabilizing or coating agent for NPs [123,126,127]. This neutral, hydrophilic and biocompatible polymer has been approved by the Food and Drug Administration (FDA) for biomedical and pharmaceutical applications [128]. PEG improves the AgNPs dispersity in physiological conditions by steric hindrance and prevents nanoparticles aggregation [107,115,129].…”

Section: Polymer Coatingsmentioning

confidence: 99%

“…The interaction between bio-receptors (native proteins (albumin)) and Cu 2+ ions leads to protein denaturation, inducing a lower affinity between protein-gold surface, thus initiating the competitive displacement by the native one (Figure 10a), which is monitored by SPR measurement with a LOD down to 0.1 mg/L. A mercury (II) sensor, based on the dissociation rate of the trans-acting factor MerR from the cis-element, was investigated by SPR [128]. The sensor, modified with dsDNA including the cis-element (Pmer), can monitor the dissociation stage of MerR or protein-tagged MerR from the cis-element, enabling measurement of Hg 2+ with a LOD of 5 μg/L.…”

Section: Surface Plasmon Resonancementioning

confidence: 99%

Spectroscopy-Based Biosensors

2021

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MerR‐fluorescent protein chimera biosensor for fast and sensitive detection of Hg²⁺ in drinking water

Özyurt

Üstükarcı

Evran

et al. 2019

Biotech and App Biochem

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Mercury ion (Hg2+) is a universal pollutant and its detection is crucial for public healthcare. In this study, we developed a novel fluorescent biosensor by construction of a protein fusion between the mercury‐sensing transcription factor MerR and enhanced yellow fluorescent protein (EYFP). Hg2+‐induced conformational change of MerR was transduced into fluorescence signal. Fluorescence intensity of the biosensor protein decreased with increasing concentrations of Hg2+ and a linear response was obtained in the range of 0.5–40 nM. The limit of detection was 0.5 nM, which was much lower than the maximum allowed level in water. The biosensor specificity was highly dependent on type and concentration of metal ion. The biosensor exhibited high specificity in a mixture of metal ions at 0.5 nM concentration. However, the interference effect was more pronounced at 40 nM concentration of metal ions. The measurement was completed in less than 1 Min with no need for sample preparation or preincubation steps. The biosensor achieved accurate and reliable detection in the spiked drinking water sample, as validated by the inductively coupled plasma optical emission spectrometry.

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Mercury (II) sensor based on monitoring dissociation rate of the trans-acting factor MerR from cis-element by surface plasmon resonance

Cited by 11 publications

References 24 publications

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

Spectroscopy-Based Biosensors

MerR‐fluorescent protein chimera biosensor for fast and sensitive detection of Hg²⁺ in drinking water

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Mercury (II) sensor based on monitoring dissociation rate of the trans-acting factor MerR from cis-element by surface plasmon resonance

Cited by 11 publications

References 24 publications

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

Bio-Recognition in Spectroscopy-Based Biosensors for *Heavy Metals-Water and Waterborne Contamination Analysis

Spectroscopy-Based Biosensors

MerR‐fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking water

Contact Info

Product

Resources

About

MerR‐fluorescent protein chimera biosensor for fast and sensitive detection of Hg²⁺ in drinking water