Mercury resistance and detoxification in bacteria

Belliveau, B. H.; Trevors, J. T.

doi:10.1002/aoc.590030402

Cited by 38 publications

(14 citation statements)

References 107 publications

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“…The spheroplast transformation system described in this report is similar to but not as efficient as that reported for other gram-positive bacteria (18,22,30 Satisfying transformation results were obtained by using electroporation, as has been described for other grampositive bacteria (3,19). A drop in the transformation rate when vector DNA isolated from E. coli was used indicates the presence of a restriction system in C. michiganense.…”

Section: Methodssupporting

confidence: 69%

Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector

Meletzus

Eichenlaub

1991

J Bacteriol

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We constructed a cloning vector for use in the plant pathogenic bacterium Clavibacter michiganense subsp. michiganense. The vector pDM100 consists of a 3.2-kb restriction fragment of the Clavibacter plasmid pCMl joined to a pBR325 derivative carrying the neomycin phosphotransferase of transposon TnS and the gentamicin acetyltransferase of Tnl696. Both antibiotic resistance genes are efficiently expressed in C. michiganense subsp. michiganense. Although polyethylene glycol-mediated transfection of spheroplasts with the DNA of the C. michiganense subsp. michiganense-specific bacteriophage CMP1 yielded about 3 x 103 transfectants per ,ug of DNA, in transformations with plasmid DNA only a very few transformants were obtained. However, the transformation efficiency could be improved by electroporation of intact cells, giving about 2 x 103 transformants per ,ug of plasmid DNA. Since a transformation procedure and a cloning vector are now available, pathogenicity in C. michiganense subsp. michiganense can now be analyzed genetically.

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Section: Methodssupporting

confidence: 69%

Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector

Meletzus

Eichenlaub

1991

J Bacteriol

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“…In contrast, the survival percentage decreased. The maximum value of transformation efficiency achieved is among of that reported for other gram-positives bacterium (Meletzus and Eichenlaub, 1991;Belliveau and Trevors, 1989) but it is lower than that reported for Escherichiu cofi (Dower et al, 1988). This could be due to the longer cell wall density of gram-positive bacteria when compared with that of gram-negative bacteria as have been mentioned by Trevors et al (1992).…”

mentioning

confidence: 61%

“…Electroporation offers the simplest and fastest method to transform a cell (Puyet et al, 1987). In this paper, we report the transformation of intact cells of Bacillzrs megaterium using the Belliveau and Trevors (1989) method with some modifications EXPERIMENTAL METHOD: Bacillus megaterium was grown for 16 h at 37°C in L-broth (LB) medium (1% tryptone, 0.5% yeast extract and 0.5% NaCl) and 0.5 ml of the culture was inoculated into 50 ml fresh LB. When the culture reached 1 0.D.620, the cells were collected by centrimgation at 3000 rpm for 10 min, washed once with electroporation buffer (25% PEG 6000 and 0.1 M sorbitol) and resuspended into 1 ml of the same buffer.…”

mentioning

confidence: 99%

Transformation of Bacillus megaterium by electroporation

Moro

Sánchez²,

Serguera

1995

Biotechnol Tech

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“…Organomercurial lyase (merB gene product) catalyzes cleavage of C-Hg bonds in organomercurial compounds to release mercuric ions, which are enzymatically reduced to less toxic and more volatile metallic mercury (Hg°) by merA-product mercuric reductase (12,(14)(15)(16)(17). Cysteine-rich transport proteins originated from merP and merT genes locate on the periplasmic space and inner membrane, respectively.…”

Section: Bioprocess Development For Microbial Mercury Detoxificationmentioning

confidence: 99%

Bioprocess Development for Mercury Detoxification and Azo-Dye Decolorization

Chang¹

2003

ACS Symposium Series

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Biological treatment of environmental pollutants is often very complex and requires intensive control of operation parameters. Fermentation of microorganisms able to degrade or destroy pollutants is a key technology to ensure appropriate cell concentration and populations are maintained in the treatment system. Optimal operation strategies, especially policy for substrates feeding and oxygen supply, need to be developed to enhance the rates of biodegradation or biotransformation for efficient cleanup of the target pollutants. In this chapter, two unique types of biological treatment processes, namely, mercury detoxification and azo-dye decolorization, are introduced as the example to show how fermentation technology and bioreactor approaches are used to develop bioprocesses for environmental applications. Detoxification of mercury is conducted aerobically using wild--type and recombinant strains as the biocatalyst. The performance and stability of fed-batch and chemostat bioreactors for mercury detoxification are greatly correlated to the dosage of the toxic mercury substrate. In contrast, azo dye is less toxic but decolorization of azo dyes requires aerobic/anaerobic sequential environments. Thus, bioreactor design for bacterial decolorization focused on strategies for the supply of oxygen and retention of high cell concentration within the reactors to achieve optimal combinantion of aerobic cell growth and anaerobic decolorization. The effectiveness and feasibility of various bioreactor configurations and strategies are assessed.

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Mercury resistance and detoxification in bacteria

Cited by 38 publications

References 107 publications

Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector

Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector

Transformation of Bacillus megaterium by electroporation

Bioprocess Development for Mercury Detoxification and Azo-Dye Decolorization

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