Merkel cell development in the wound healing in the labial mucosa of adult rabbits.

Tachibana, Tamiko; Ishizeki, Kiyoto

doi:10.1679/aohc1950.44.151

Cited by 30 publications

(16 citation statements)

References 19 publications

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“…Although the length of the experimental period seems relatively short in this study, 7 days is a sufficient observation period, since Merkel cells had not regenerated within 3 months after wounding or 14 days after implantation in pilot experiments (unpublished data). However, in contrast to our results, Tachibana and Ishizeki reported that Merkel cells did regenerate after partial resection of the labial skin of rabbits (25). This difference in the regenerative behavior of Merkel cells between the skin and the oral mucosa might be caused by differences in the embryonic environment.…”

Section: Regeneration Capacity Of Merkel Cellscontrasting

confidence: 99%

“…In addition, the relationship between nerve fibers and Merkel cells has also been reported (21,23). However, there have been no reports about the behavior of Merkel cells around dental implants in the palatine mucosa of any kind of animal, although Tachibana and Ishizeki studied Merkel cell behavior related to wound healing in rabbit skin (25). In the palatine mucosa of hamsters, it is known that the number of Merkel cells in the fourth palatine ruga is the highest and that the number in the third palatine ruga is the next highest (12).…”

Section: Discussionmentioning

confidence: 96%

“…A study of the behavior of Merkel cells and nerve endings after partial excision of the lip has shown that Merkel cells and nerve fibers are regenerated (25). However, there have been no reports on the behavior of Merkel cells and/or nerve endings in re-drilling with a #60 dental K-file.…”

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Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

et al. 2005

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The purpose of this study was to investigate the relationship between Merkel cells and nerve elements during tissue regeneration after receiving dental implants. Golden hamsters were divided into 3 groups and titanium alloy implants were fixed in their left-side maxilla through the third palatine ruga. Animals were sacrificed at 1, 2, 3, 4, 5, 6, and 7 days after the implantation and tissues were characterized at the immunohistochemical and morphological levels. CK 20 and PGP 9.5 antibodies which react with Merkel cells and nerve fibers were used. Immunohistochemically, no CK20-positive Merkel cells were seen in the peri-implant epithelium throughout the 7 days. However, starting at day 4, PGP 9.5-positive nerve fibers appeared in the connective tissue, and by day 7, nerve fibers had invaded the more superficial layer of the peri-implant epithelium compared to the mucosa removal control group. At the electron microscopic level, the intercellular spaces of the regenerating epithelium in the mucosa removal control group were small. In contrast, intercellular spaces of the peri-implant epithelium tended to be wide and regenerating nerve fibers invaded those intercellular spaces. In both the mucosa removal control group and the implantation group, the basal lamina and connective tissues regenerated completely. However, clear Merkel cells containing neurosecretory granules were not observed. Taken together, our results indicate that Merkel cells in the hamster palatine mucosa do not regenerate in the peri-implant epithelium. However, regenerative nerve fibers seem to play essential roles as part of the defense and sensory systems around the peri-implant epithelium to compensate for the weakened defense mechanism.It is well known that Merkel cells play an important role in tissues as a sensory system for pressure (8,16,28). Previous studies have revealed that Merkel cells are widely distributed in human oral mucosal tissues including the lip, hard palate and mandibular gingiva (1, 13). A fundamental, ultrastructural study of rodent oral mucosa demonstrated that two types of Merkel cells, with dendritic or roundish shapes, are recognized. Most dendritic Merkel cells are free of innervations while the roundish ones are innervated (26). In addition, Merkel cell-neurite complexes are considered to be slowly adapting type I mechanoreceptors in the oral mucosa of the hamster cheek pouch (28,29).The behavior of Merkel cells after denervation has also been elucidated (22), the conclusion being that Merkel cells survive with normal density and appearance in the epidermis after denervation. A study of the behavior of Merkel cells and nerve endings after partial excision of the lip has shown that Merkel cells and nerve fibers are regenerated (25). However, there have been no reports on the behavior of Merkel cells and/or nerve endings in re-

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Section: Regeneration Capacity Of Merkel Cellscontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 96%

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Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

et al. 2005

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“…Part of the lower labial mucosa was excised following mental nerve resection. (ENGLISH, 1974;OCHIAI, 1977;OCHIAI et al, 1979;TACHIBANA, 1979;ENGLISH et al, 1980;OCHIAI and SuzuKI, 1981;TACHIBANA and ISHIZEKI, 1981). However, the mechanism of differentiation of the Merkel cell is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, there is much circumstantial evidence that a very immature Merkel cell has no connection with an intraepithelial nerve element (LYNE and HOLLIS, 1971;OcHIAI et al, 1979;TACHIBANA, 1979;ENGLISH et al, 1980;OCHIAI and SUZUKI,1981;TACHIBANA and ISHIZEKI., 1981). Furthermore, in amphibia, Merkel cells were found in the skin of the aneurogenic embryo and in the regenerated skin of the denervated adult animal (TWEEDLE,1978;SCOTT et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

An experimental study of the influence of sensory nerve fibers on Merkel cell differentiation in the labial mucosa of the rabbits.

Tachibana

Sakakura

Ishizeki

et al. 1983

Arch. Histol. Jap., Arch. Histol. Jpn

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Degeneration of mouse digital corpuscles

Idé

1982

Am. J. Anat.

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The sequential changes of the morphology and the histochemically demonstrated, nonspecific cholinesterase (ChE) activity of the denervated digital corpuscle (Meissner corpuscle) of the mouse were observed by light and electron microscopy for 6 months after transection of the sciatic nerve. A fragment of the sciatic nerve, approximately 1 cm long, was removed from the distal stump to prevent reinnervation. Within one day following nerve division, the axon terminals began to manifest degenerative changes: the axoplasm became electron-opaque and some mitochondria became disorganized. The lamellar cell processes (lamellar) also exhibited atrophic changes: they became thinner and more electron opaque. The lamellar cell body became smaller due to a decreased amount of cytoplasm and contained few organelles except for some free ribosomes. These changes advance with time. Around 4-6 months after denervation, the corpuscle was small, consisting of a few thin, shrunken, atrophic lamellar cells. The ChE activity persisted and could be demonstrated in the interlamellar spaces, but its intensity decreased with time so that the corpuscle was hardly identifiable by light microscope histochemistry around 4-6 months after denervation. During this time, the precipitates of the reaction product were scattered as small particles throughout the interlamellar spaces. The enzyme activity in the cisternae of the rough endoplasmic reticulum and the nuclear envelope of the lamellar cell body was no longer demonstrable 5 days or less after denervation. These findings indicate that the lamellar cell is dependent on the axon terminal to maintain its normal morphology and the function of ChE synthesis.

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Merkel cell development in the wound healing in the labial mucosa of adult rabbits.

Cited by 30 publications

References 19 publications

Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

Characterization of the peri-implant epithelium in hamster palatine mucosa: Behavior of Merkel cells and nerve endings

An experimental study of the influence of sensory nerve fibers on Merkel cell differentiation in the labial mucosa of the rabbits.

Degeneration of mouse digital corpuscles

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