Merocyanine 540 as a probe to monitor the molecular packing of phosphatidylcholine: a monolayer epifluorescence microscopy and spectroscopy study

Yu, Hao; Hui, Sek Wen

doi:10.1016/0005-2736(92)90411-e

Cited by 36 publications

(28 citation statements)

References 28 publications

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“…This argument explains the subtlety of spectral shape changes in Fig. 3A, because the spectral peak at about 620 nm is reported to depend on the perpendicular orientation (16).…”

Section: Liposomesmentioning

confidence: 72%

“…When lipids are more loosely packed and the intermolecular spacing is therefore greater, the probe displays a maximum intensity at about 585 nm with a large increase in quantum yield (15). The spectral differences observed between MC540 bound to tightly or loosely packed lipids may relate to alterations in probe orientation from a position parallel to the membrane surface to one perpendicular and immersed among lipid chains as inter-lipid spacing increases (16).…”

mentioning

confidence: 99%

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Use of fluorescence to determine the effects of cholesterol on lipid behavior in sphingomyelin liposomes and erythrocyte membranes

Stott

McLemore

et al. 2008

Journal of Lipid Research

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The purpose of this study was to generate the equivalent of a cholesterol/temperature phase map for a biological membrane using fluorescence spectroscopy. The pseudo-phase map was created using human erythrocytes treated with various concentrations of methyl-b-cyclodextrin to remove defined amounts of cholesterol and a trio of fluorescent probes that assess different membrane properties (laurdan, diphenylhexatriene, and merocyanine 540). Parallel experiments with two-photon microscopy suggested that changes in cellular cholesterol content affected the entire membrane rather than being localized to specific macroscopic domains. The various regions of the composite erythrocyte pseudo-phase map were interpreted using analogous data acquired from multilamellar vesicles that served as simplified models of cholesterol-dependent phases. The vesicles consisted of various concentrations of cholesterol (0 to 50 mol%) with either palmitoyl sphingomyelin, 1:1 dipalmitoylphosphatidylcholine and dioleoylphosphatidylcholine, or phospholipid mixtures intended to simulate either the inner or outer leaflet of erythrocyte membranes. Four distinguishable regions were observed in sphingomyelin phase maps corresponding to the traditional solid-ordered and liquiddisordered phases and two types of liquid-ordered behavior. Physical properties were less diverse in the mixed phospholipid vesicles, as expected, based on previous studies. Erythrocytes displayed five regions of different combinations of membrane properties along the phase map. Some of the observations identified similarities between the cells and liquidordered behavior observed in the various types of liposomes as well as some interesting

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“…This argument explains the subtlety of spectral shape changes in Fig. 3A, because the spectral peak at about 620 nm is reported to depend on the perpendicular orientation (16).…”

Section: Liposomesmentioning

confidence: 72%

mentioning

confidence: 99%

Use of fluorescence to determine the effects of cholesterol on lipid behavior in sphingomyelin liposomes and erythrocyte membranes

Stott

McLemore

et al. 2008

Journal of Lipid Research

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“…For personal use only. on May 12, 2018. by guest www.bloodjournal.org From Cell shrinkage and MC540 uptake by murine B cells MC540 is a fluorescent, lipophilic dye that binds preferentially to the outer leaflet of plasma membrane bilayers with relatively loosely packed lipids, 5,[18][19][20] including those of apoptotic cells. 6,7 We compared the kinetic relationship between MC540-binding (to measure lipid packing) and AV-binding (to measure PS exposure).…”

Section: Ps Translocation By B Cells Is Independent Of Abca1mentioning

confidence: 99%

Phosphatidylserine exposure in B lymphocytes: a role for lipid packing

Elliott

Sardini

Cooper

et al. 2006

Blood

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Plasma membrane lipids are usually distributed asymmetrically, with phosphatidylserine (PS) confined to the inner leaflet. PS exposure at the outer leaflet occurs early in apoptosis, but it is also constitutive on some nonapoptotic cell populations where it plays a role in cell signaling. How PS is transported ("flopped") to the cell surface is unknown. Contrary to previous reports that normal murine B lymphocytes lack lipid asymmetry, we show that PS is normally restricted to the inner leaflet of these cells. PS exposure on normal B cells did, however, occur spontaneously ex vivo. Consistent with the hypothesis that loss of PS asymmetry is regulated by CD45, PS is constitutively exposed on viable, CD45-deficient B cells. We show that calcium-stimulated PS exposure in B cells is strain variable, ABCA1 independent, and both preceded by and dependent on a decrease in lipid packing. This decrease in lipid packing is concomitant with cell shrinkage and consequent membrane distortion, both of which are potently inhibited by blockers of volumeregulatory K ؉ and Cl ؊ ion channels. Thus, changes in plasma membrane organization precede PS translocation. The data suggest a model in which PS redistribution may occur by a translocaseindependent mechanism at energetically favorable sites of membrane perturbation where lipid packing is decreased. (Blood.

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“…Figure 5 shows that the uptake of 3H- (Sheetz & Singer, 1974). It displays differential partitioning into membranes and bilayers, depending on the lipid packing density in the outer leaflet (Williamson et al, 1983), and has been used to monitor the molecular packing of phospholipid monolayers (Yu & Hui, 1992). A series of MDR cell lines was treated with MC540 and analysed for dye retention using flow cytometry.…”

Section: Effect Of Detergent Structure On Collateral Sensitivitymentioning

confidence: 99%

Interaction of multidrug-resistant Chinese hamster ovary cells with amphiphiles

Loe

Sharom²

1993

Br J Cancer

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Summary The interaction of membrane-active amphiphiles with a series of MDR Chinese hamster ovary (CHO) cell lines was investigated. Cross-resistance to cationic amphiphiles was observed, which was effectively sensitised by verapamil. MDR cells showed collateral sensitivity to polyoxyethylene amphiphiles (Triton X-100/Nonidet P40), which reached a maximum at 9-10 ethylene oxide units. Resistant lines were also highly collaterally sensitive (17-fold) to dibutylphthalate. mdrl transfectants showed cross-resistance to cationic amphiphiles, but no collateral sensitivity to nonionic species. Triton X-100/Nonidet P-40 inhibited 3H-azidopine photoaffinity labelling at low concentrations, perhaps reflecting a specific interaction with Pglycoprotein.Further investigation of the molecular basis of collateral sensitivity revealed that association of 3H-Triton X-100 with MDR cells reached steady state levels rapidly, and occurred by a non-mediated mechanism. The equilibrium level of X-100 uptake was inversely related to drug resistance. Collateral sensitivity is thus not a result of decreased Triton X-100 association with the cell.The fluorescent probe merocyanine 540 was used to examine the MDR plasma membrane microenvironment for physicochemical changes. Increasing levels of drug resistance correlated with a progressive shift in the mean cell fluorescence to lower levels, which suggests that the packing density in the outer leaflet of MDR cells is increased relative to that of the drug-sensitive parent.

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Merocyanine 540 as a probe to monitor the molecular packing of phosphatidylcholine: a monolayer epifluorescence microscopy and spectroscopy study

Cited by 36 publications

References 28 publications

Use of fluorescence to determine the effects of cholesterol on lipid behavior in sphingomyelin liposomes and erythrocyte membranes

Use of fluorescence to determine the effects of cholesterol on lipid behavior in sphingomyelin liposomes and erythrocyte membranes

Phosphatidylserine exposure in B lymphocytes: a role for lipid packing

Interaction of multidrug-resistant Chinese hamster ovary cells with amphiphiles

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