Meroterpenes from Psoralea corylifolia against Pyricularia oryzae

Huang, Youwu; Liu, Xiaoyu; Wu, Yingchun; Li, Yiming; Guo, Fujiang

doi:10.1055/s-0034-1382995

Cited by 20 publications

(7 citation statements)

References 23 publications

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“…It is widely used to treat yang deficiency of the spleen and kidney in adult and pediatric patients in China, Korea, and Japan. Furthermore, modern pharmacological studies have confirmed that this seed exhibits potent medicinal activities like estrogenic (Liu et al, 2020), antitumor (Jin et al, 2020), anti-oxidant (Limper et al, 2013), antimicrobial (Huang et al, 2014), anti-inflammatory (Szliszka et al, 2011), antidepressant (Mao et al, 2014), and osteoblastic activities (Huang et al, 2019). However, recent clinical reports have indicated that treatment associated with PF has an increased risk of liver injury (Nam et al, 2005;Teschke and Bahre, 2009;Smith and MacDonald, 2014;Li and Huang, 2016;Li and Cheng, 2018;Zhang and Huang, 2018).…”

Section: Introductionmentioning

confidence: 99%

Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis

et al. 2021

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Backgrounds: Psoraleae Fructus (PF)-induced hepatotoxicity has been reported in clinical and animal experiments. However, the hepatotoxic constituents and mechanisms underlying PF-induced toxicity have remained unclear. Therefore, this study explored the potentially toxic PF components and revealed their relative mechanisms.Methods: The hepatotoxicity of PF water (PFW) and ethanol (PFE) extracts was compared using Kunming mice. The different compositions between PFW and PFE, which were considered toxic compositions, were identified using the UHPLC-Q-Exactive MS method. Then, L02 and HepG2 cell lines were used to evaluate the toxicity of these compositions. Cell viability and apoptosis were determined through the Cell Counting Kit-8 (CCK-8) assay and flow cytometry, respectively. An automatic biochemical analyzer detected the aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Lastly, we used high-content screening (HCS) to determine the levels of reactive oxygen species (ROS), lipid, and mitochondrial membrane potential (MMP).Results: The ethanol extraction process aggravated the hepatotoxicity of PF, causing more severe injuries. The content of psoralen, isopsoralen, bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol was higher in the PFE than PFW. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol induced cell apoptosis and the AST, ALT, and ALP leakages. Furthermore, these five constituents increased intracellular lipid accumulation and ROS levels but decreased the MMP level.Conclusion: The ethanol extraction process could induce severe PF hepatotoxicity. Bavachin, psoralidin, bavachinin, neobavaisoflavone, and bakuchiol are the main hepatotoxic ingredients. This mechanism could be associated with oxidative stress and mitochondrial damage-mediated apoptosis. Taken together, this study provides a basis for the clinical application of PF that formulates and improves its herbal standards.

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Section: Introductionmentioning

confidence: 99%

Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis

et al. 2021

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“…Psoralea corylifolia L. (PC), belonging to Leguminosae, is one of the most popular traditional Chinese medicines used for psoriasis and vitiligo [8,9]. Phytochemical researches show that P. corylifolia contains coumarins, flavonoids, and monoterpene phenols [10,11]. As for the polysaccharides of P. corylifolia , the studies on polysaccharides from P. corylifolia purification and their structural characteristics and biological activities for P. corylifolia polysaccharides were few, and only a few scholars have conducted preliminary studies, for example Zheng C X et al [12] initially studied the feasibility and the mechanism of the polysaccharide from P. corylifolia for the repair of articular cartilage defects in rabbits.…”

Section: Introductionmentioning

confidence: 99%

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Yin

Zhang

et al. 2019

Molecules

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Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L. Their average molecular weights were 2.721 × 104 and 2.850 × 104. PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different. Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified. The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved. The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability. Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 μM) in vitro.

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“…Bakuchiol is a meroterpene, which can be found in the traditional Chinese herbal medicine Fructus Psoraleae , the dried ripe fruit of Psoralea corylifolia L. (Fabaceae). It is shown to have anti-microbial, anti-inflammatory, anti-oxidative, anti-osteoporosis, and anti-depression or anti-stress activities ( Lim et al, 2009 ; Choi et al, 2010 ; Kim et al, 2013 ; Huang et al, 2014 ; Mao et al, 2014 ). The estrogenic activities of bakuchiol were reported in several in vitro models ( Xin et al, 2010 ; Lim et al, 2011 ; Mao et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Phytoestrogen Bakuchiol Exhibits In Vitro and In Vivo Anti-breast Cancer Effects by Inducing S Phase Arrest and Apoptosis

Chen

Liu

et al. 2016

Front. Pharmacol.

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Phytoestrogen has been proposed as an alternative to hormone replacement therapy, which has been demonstrated to promote a high risk of breast cancer. However, the effect of phytoestrogen on breast cancer development has not been fully understood. Bakuchiol is an active ingredient of a traditional Chinese herbal medicine Fructus Psoraleae, the dried ripe fruit of Psoralea corylifolia L. (Fabaceae). The in vitro and in vivo estrogenic activities and anti-breast cancer effects of bakuchiol have not been well-studied. We found that bakuchiol induced the GFP expression in transgenic medaka (Oryzias melastigma, Tg, Chg:GFP) dose-dependently (0–1 μg/ml), demonstrating its in vivo estrogenic activity. Low dose of bakuchiol (1 μg/ml) induced the cell proliferation and ERα expression in MCF-7 cells, which could be blocked by the anti-estrogen ICI 182780, suggesting the in vitro estrogenic activity of bakuchiol. Our data indicated that high doses of bakuchiol (>2 μg/ml) inhibited breast cancer cell growth, with a stronger anti-proliferative effect than resveratrol, a widely studied analog of bakuchiol. High doses of bakuchiol (4, 7, and 10 μg/ml) were used for the further in vitro anti-breast cancer studies. Bakuchiol induced ERβ expression and suppressed ERα expression in MCF-7 cells. It also induced S phase arrest in both MCF-7 and MDA-MB-231 cells, which could be rescued by caffeine. Knock-down of p21 also marginally rescued S phase arrest in MCF-7 cells. The S phase arrest was accompanied by the upregulation of ATM, P-Cdc2 (Tyr15), Myt1, P-Wee1 (Ser642), p21 and Cyclin B1, suggesting that blocking of Cdc2 activation may play an important role in bakuchiol-induced S phase arrest. Furthermore, bakuchiol induced cell apoptosis and disturbed mitochondrial membrane potential in MCF-7 cells. The bakuchiol-induced apoptosis was associated with increased expression of Caspase family and Bcl-2 family proteins, suggesting that bakuchiol may induce apoptosis via intrinsic apoptotic pathway. The in vivo anti-breast cancer effect of bakuchiol was further proved in zebrafish (Danio rerio, wild-type AB) xenografts. 0.5 μg/ml of bakuchiol significantly reduced the MCF-7 cell mass in zebrafish xenografts. Overall, these results suggested the potential of using bakuchiol in HRT and breast cancer treatment.

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Meroterpenes from Psoralea corylifolia against Pyricularia oryzae

Cited by 20 publications

References 23 publications

Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis

Five Constituents Contributed to the Psoraleae Fructus-Induced Hepatotoxicity via Mitochondrial Dysfunction and Apoptosis

Two Novel Polysaccharides in Psoralea corylifolia L and anti-A549 Lung Cancer Cells Activity In Vitro

Phytoestrogen Bakuchiol Exhibits In Vitro and In Vivo Anti-breast Cancer Effects by Inducing S Phase Arrest and Apoptosis

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