Mesenchymal precursor cells in the blood of normal individuals

Zvaifler, Nathan J.; Marinova-Mutafchieva, Lilla; Adams, Gill; Edwards, Christopher J; Moss, Jill; Burger, Jan A.; Maini, R. N.

doi:10.1186/ar130

Cited by 635 publications

(352 citation statements)

References 51 publications

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“…4 and 13-27.) Also, several reports have described isolation of precursor cells for nonhematopoietic tissues from peripheral blood (28)(29)(30). However, several recent publications have challenged some of the evidence that bone marrow contains cells that can repair nonhematopoietic tissues.…”

Section: Reparative Cells From Bone Marrow: Data From Experimental Anmentioning

confidence: 99%

One strategy for cell and gene therapy: Harnessing the power of adult stem cells to repair tissues

Prockop

Gregory

Spees

2003

Proc. Natl. Acad. Sci. U.S.A.

386

283

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Most recent evidence suggests that the process of tissue repair is driven by stem-like cells that reside in multiple tissues but are replenished by precursor cells from bone marrow. Among the candidates for the reparative cells are the adult stem cells from bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). We recently found that after MSCs were replated at very low densities to generate single-cell-derived colonies, they did not exit a prolonged lag period until they synthesized and secreted considerable quantities of Dickkopf-1, an inhibitor of the canonical Wnt signaling pathway. We also found that when the cells were cocultured with heat-shocked pulmonary epithelial cells, they differentiated into epithelial cells. Most of the MSCs differentiated without evidence of cell fusion but up to one-quarter underwent cell fusion with the epithelial cells. A few also underwent nuclear fusion. The results are consistent with the interesting possibility that MSCs and similar cells repair tissue injury by three different mechanisms: creation of a milieu that enhances regeneration of endogenous cells, transdifferentiation, and perhaps cell fusion.

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Section: Reparative Cells From Bone Marrow: Data From Experimental Anmentioning

confidence: 99%

One strategy for cell and gene therapy: Harnessing the power of adult stem cells to repair tissues

Prockop

Gregory

Spees

2003

Proc. Natl. Acad. Sci. U.S.A.

386

283

View full text Add to dashboard Cite

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“…(7, derivatives of all three germ layers (10), but the adult stem cells may have less replication and differentiation 11,12,20,21,25,32). Current investigation on the immunophenotype and differentiation potential of second tricapacities.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Neural Differentiation of Fetal Lung Mesenchymal Stem Cells

et al. 2005

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Mesenchymal stem cells (MSCs) have been successfully isolated from a broad range of adult, fetal, and other nonembryonic tissues. Fetal lung has been identified as a rich source of MSCs. However, the biological characteristics and differentiation potential of fetal lung MSCs remain to be explored. In this study, we established a series of methods for isolation and expansion of fetal lung MSCs. These MSCs could withstand more than 40 passages without obvious decline in proliferation ability, significant changes in morphology, and expression of cell markers. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC, but not CD14, CD31, CD34, CD38, CD41a, CD42b, CD45, CD49d, CD61, CD106, CD133, and HLA-DR. Cell cycle analysis revealed that when the MSCs reached their log phase of growth, more than 90% of the cells were in G 0 /G 1 phase while the proportion of cells in S phase and G 2 /M phase were about 5.56% and 2.08% cells, respectively. These MSCs could differentiate into neural cells in addition to their mesenchymal differentiation potential. Our data suggest that the fetal lung MSC population is an alternative source of stem cells for cell-based therapy of neurological defects or mesenchymal-originating diseases.Key words: Mesenchymal stem cell; Bone marrow; Fetal lung; Stem cell plasticity; Neural differentiation INTRODUCTIONduced and terminally differentiated into osteoblasts, chondrocytes, adipocytes, hypocytes, tenocytes, myotubes, neural cells, and hematopoietic-supporting stroma (3,13, Self-renewal capacity, long-term viability, and multilineage potential are the basic properties of stem cells 16,18,19,21,22,31). The multipotential capability of these cells, their straightforward isolation and culture as well (8). Within stem cell research, multilineage potential embryonic stem cells and mesenchymal stem cells (MSCs) as high ex vivo expansive potential make the MSCs an attractive therapeutic tool for various kinds of diseases. from adults and fetuses are the two fields that have been extensively investigated. Embryonic stem cells, whichTo date, MSCs have been isolated from a broad range of tissues and organs, including bone marrow, umbilical are derived from mammalian embryos in the blastocyst stage, can proliferate indefinitely and differentiate into cord blood, the wall of artery, umbilical cord vein, peripheral blood, fetal bone marrow, liver, spleen, etc. (7, derivatives of all three germ layers (10), but the adult stem cells may have less replication and differentiation 11,12,20,21,25,32). Current investigation on the immunophenotype and differentiation potential of second tricapacities. Until recently, it was thought that tissuespecific adult stem cells could only differentiate into mester bone marrow, liver, lung, and spleen revealed that cultured-expanded cells from these tissues are phecells of their origin. However, current studies have suggested that tissue-specific stem cells may have greater notypically similar but exhibit heterogenei...

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“…Similar cells have been isolated from different mesenchymal tissues, including synovia (De Bari et al, 2003), tendons (Salingcarnboriboon et al, 2003), skeletal muscles (Bosch et al, 2000) and adipose tissue (Zuk et al, 2001Erickson et al, 2002), including the fat pad of the knee joint (Dragoo et al, 2003). These MSCs were also found in peripheral blood (Kuznetsov et al, 2001); however, their frequency is probably extremely low or variable (Zvaifler et al, 2000) and they are characterized by different cell surface markers (Table 1). However, no characteristic phenotype is available for specific stem cell differentiation potentials (Fibbe, 2002).…”

Section: Bone Marrow-derived Stromal Stem Cellsmentioning

confidence: 85%