Mesenchymal stem cell-conditioned medium promotes MDA-MB-231 cell migration and inhibits A549 cell migration by regulating insulin receptor and human epidermal growth factor receptor 3 phosphorylation

Abstract

Various in vitro and in vivo studies have linked mesenchymal stem cells (MSCs) with cancer, but little is known about the effect of MSCs on tumor progression. The present study aimed to analyze the role of the MSCs from different tissues, consisting of human bone marrow, adipose and the umbilical cord tissues, and the heterogeneity of tumors in tumor progression. By collecting the culture supernatants of MSCs as MSC-conditioned media (CMs), the present study found that MSC-CM produces no significant effect on the proliferation of MDA-MB-231 and A549 tumor cells. The migration of MDA-MB-231 cells was enhanced upon incubation with MSC-CM, while that of A549 cells was inhibited. Furthermore, the phosphorylation of insulin receptors (IRs) was upregulated in MSC-CM-treated MDA-MB-231 cells, while in MSC-CM-treated A549 cells, the phosphorylation of human epidermal growth factor receptor 3 (Her3) was downregulated. Taken together, the findings suggest that the phosphorylation of IR and Her3 may contribute to the discrepant effects of MSC-CM on the migration of the 2 cell lines.

Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner In Vitro

“…With regards to melanoma cells, the previous literature suggested that a line of umbilical cord (UC)-MSC could inhibit the proliferation, induce apoptosis, and suppress the invasion of A375 cells [37]. Such discordance and other comparisons between our findings and previous reports of similar studies assessing the effect of umbilical cord-derived MSC on melanoma and breast cancer cell lines [38][39][40][41][42][43][44][45][46] are summarized in Table S2. Discrepancies may be due, in part, to alternative methods for evaluating cancer cell proliferation, in addition to differing culture systems, or may be due to the heterogeneity in MSC lines utilized across various studies, as the present study would suggest.…”

Human Wharton's Jelly Stem Cell (hWJSC) Extracts Inhibit Ovarian Cancer Cell Lines OVCAR3 and SKOV3 in vitro by Inducing Cell Cycle Arrest and Apoptosis

“…This indicates that the hWJSC extracts had the potential to inhibit ovarian cancer cell motility which can be helpful to prevent metastasis. (28) reported that the conditioned medium derived from MSCs inhibited migration of lung cancer cells (A549) by decreasing the phosphorylation of human epidermal growth factor 3 (28). It is possible that hWJSC-CM and hWJSC-CL inhibited ovarian cancer cell migration by similar mechanism.…”

Exosomes from Nischarin-Expressing Cells Reduce Breast Cancer Cell Motility and Tumor Growth

“…The ECM stimulates PI3K activity, which results in cancer phenotypes, such as anchorage-independent growth on soft agar and protection from apoptosis (24,25). Previous reports show that culturing noncancerous cells in conditioned media from cancer cells promotes proliferation and migration of noncancerous cells (26,27). We previously determined that incubating noncancerous MCF10A cells with media from 231 cells promotes cell migration (28).…”