Mesenchymal stem cell-encapsulated cellulose nanofiber microbeads and enhanced biological activities by hyaluronic acid incorporation

Goh, MeeiChyn; Tae, Giyoong

doi:10.1016/j.carbpol.2021.119026

Cited by 6 publications

(2 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrophilic domains together with the specialized binding domains of cellulose allow MSC to attach and spread along the nanofibrillar network of the hydrogels during encapsulation. To that end, the material does not require any peptide sequence surface modifications to promote cell adhesion, such as incorporation of the arginine-glycine-aspartate (RGD) which has been commonly used to enhance biomaterial cell adhesion properties. , This adhesion and spreading of MSCs on the CNFs was also reported in another study using a commercial CNF based hydrogel, but in other studies using human mesenchymal and murine embryonic stem cells, , encapsulated in different types of CNF-based hydrogels, it was not observed. 3D-matrix adhesion patterns are different from the 2D in vitro substrate adhesions in terms of localization, function, and structure and therefore can potentially be more physiologically relevant …”

Section: Discussionmentioning

confidence: 95%

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

Nikolits

Radwan

Liebner

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are the most prominent type of adult stem cells for clinical applications. Three-dimensional (3D) cultivation of MSCs in biomimetic hydrogels provides a more physiologically relevant cultivation microenvironment for in vitro testing and modeling, thus overcoming the limitations of traditional planar cultivation methods. Cellulose nanofibers are an excellent candidate biomaterial for synthesis of hydrogels for this application, due to their biocompatibility, tunable properties, availability, and low cost. Herein, we demonstrate the capacity of hydrogels prepared from 2,2,6,6tetramethylpiperidine-1-oxyl -oxidized and subsequently individualized cellulose-nanofibrils to support physiologically relevant 3D in vitro cultivation of human MSCs at low solid contents (0.2−0.5 wt %). Our results show that MSCs can spread, proliferate, and migrate inside the cellulose hydrogels, while the metabolic activity and proliferative capacity of the cells as well as their morphological characteristics benefit more in the lower bulk cellulose concentration hydrogels.

show abstract

Section: Discussionmentioning

confidence: 95%

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

Nikolits

Radwan

Liebner

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…64,65 Natural polymers with biocompatibility and biodegradability are ideal options for the fabrication of ECM-mimicking hydrogels. 66 To overcome the challenge of uncontrolled degradation of natural hydrogels, many strategies have been developed, such as hybridization, compounding and modification. For example, Ashton et al fabricated an alginate/poly(lactideco-glycolide) hybrid hydrogel with controllable and tunable enzymatical degradation.…”

Section: Degradationmentioning

confidence: 99%

Advances in hydrogels for stem cell therapy: regulation mechanisms and tissue engineering applications

et al. 2022

View full text Add to dashboard Cite

show abstract

Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

Zheng,

Tian,

et al. 2024

Small

View full text Add to dashboard Cite

Stem cell‐related therapeutic technologies have garnered significant attention of the research community for their multi‐faceted applications. To promote the therapeutic effects of stem cells, the strategies for cell microencapsulation in hydrogel microparticles have been widely explored, as the hydrogel microparticles have the potential to facilitate oxygen diffusion and nutrient transport alongside their ability to promote crucial cell‐cell and cell‐matrix interactions. Despite their significant promise, there is an acute shortage of automated, standardized, and reproducible platforms to further stem cell‐related research. Microfluidics offers an intriguing platform to produce stem cell‐laden hydrogel microparticles (SCHMs) owing to its ability to manipulate the fluids at the micrometer scale as well as precisely control the structure and composition of microparticles. In this review, the typical biomaterials and crosslinking methods for microfluidic encapsulation of stem cells as well as the progress in droplet‐based microfluidics for the fabrication of SCHMs are outlined. Moreover, the important biomedical applications of SCHMs are highlighted, including regenerative medicine, tissue engineering, scale‐up production of stem cells, and microenvironmental simulation for fundamental cell studies. Overall, microfluidics holds tremendous potential for enabling the production of diverse hydrogel microparticles and is worthy for various stem cell‐related biomedical applications.

show abstract

Mesenchymal stem cell-encapsulated cellulose nanofiber microbeads and enhanced biological activities by hyaluronic acid incorporation

Cited by 6 publications

References 78 publications

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

Hydrogels from TEMPO-Oxidized Nanofibrillated Cellulose Support In Vitro Cultivation of Encapsulated Human Mesenchymal Stem Cells

Advances in hydrogels for stem cell therapy: regulation mechanisms and tissue engineering applications

Droplet Microfluidics Powered Hydrogel Microparticles for Stem Cell‐Mediated Biomedical Applications

Contact Info

Product

Resources

About