Mesenchymal stem cell therapy of brain ischemic stroke in rats

Zin’kova, N. N.; Gilerovich, E. G.; Sokolova, I. B.; Viide, S. K.; Shvedova, E. V.; Alexandrov, Georgy; Kruglyakov, P. V.; Polyntsev, D. G.

doi:10.1134/s1990519x07050033

Cited by 5 publications

(6 citation statements)

References 30 publications

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“…One or two nucleoli are distinctly visible in the nucleus. These morphological characteristics of the MSCs are in full accordance with those described by other authors [3,8,10].…”

Section: Resultssupporting

confidence: 91%

“…MSCs are known to have a high migration potential. Their migration has been described after MSCs transplantation into the brain [3]. They affect axonal regeneration after transplantation into nerve or nerve conduit (bridge nerve gap) or after intravenous administration [18].…”

Section: Resultsmentioning

confidence: 99%

“…MSCs derived from bone marrow of Wistar-Kyoto rats were provided by "Trans-Technologies" (Russia). The method of obtaining and characteristics of MSCs were described in more detail earlier by Zinkova et al [3]. Cells were cultured for a week, using a culture flasks (NEST Scientific, USA) and culture medium MEM alpha (BioloT, Russia) supplemented with bovine serum.…”

Section: Mscsmentioning

confidence: 99%

“…Current regenerative neurobiology have demonstrated that transplantation of stem cells can have a stimulating effect on the processes of reparative regeneration of the nervous system organs [1][2][3]. The experimental elaboration of cellular technologies to stimulate nerve regeneration is carried out actively [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Rat mesenchymal stem cells differentiate to endothelial cells after allotransplantation into the damaged nerve

Петрова¹,

Исаева²,

Коржевский³

2016

Transplant Open

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At present, achievements in regenerative neurobiology have demonstrated that transplantation of different kinds of stem cells can have a stimulating effect on the processes of reparative regeneration of the nervous system. The experimental elaboration of mesenchymal stem cells (MSCs) transplantation to stimulate nerve regeneration is carried out actively. There is evidence that MSCs promote the growth of the recipient regenerating axons after transplantation into the damaged nerve or a conduit. However, the fate of the transplanted cells and their differentiation is poorly studied. The aim of the present study was to investigate localization and differentiation of rat bone marrow MSCs 1 and 7 days after transplantation into the injured sciatic nerve of adult rats. MSCs from Wistar-Kyoto rats were cultured for 7 days and labeled with BrdU 3 days prior to usage. The sciatic nerve of adult Wistar-Kyoto rats was pinched and a suspension of BrdU-labeled cultured MSCs was immediately transplanted into a damaged sciatic nerve. 1 day after surgery, the transplanted MSCs were found in the endoneurium. 7 days after transplantation, the MSCs were found survive and were localized in the epineurium. A part of the transplanted MSCs differentiates to cells of the vessel wall; according to their morphology, these were endothelial cells. Thus, the transplanted MSCs can contribute to regeneration of the transected nerve by involvement in vascularization of the injured tissue.

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“…One or two nucleoli are distinctly visible in the nucleus. These morphological characteristics of the MSCs are in full accordance with those described by other authors [3,8,10].…”

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Mscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rat mesenchymal stem cells differentiate to endothelial cells after allotransplantation into the damaged nerve

Петрова¹,

Исаева²,

Коржевский³

2016

Transplant Open

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“…It has been suggested that venous injection of MSCs could activate processes outside the brain, causing endogenous stem cells to be stimulated, which play a role in repairing the damage [35]. The results of the current study suggest that BM-MSCs could attenuate the CP-induced neurotoxicity via a paracrine mechanism through the secretion of many factors that contribute to the reduction of oxidative stress, inflammation, and apoptosis [34,35].…”

Section: Discussionmentioning

confidence: 62%

Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy

2021

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Cisplatin (CP) is extensively used in the medical oncology field for malignancy treatment, but its use is associated with neurological side effects that compromise the patients’ quality of life. Cytotherapy is a new treatment strategy for tissue damage that has recently emerged. The use of bone marrow-derived mesenchymal stem cells (BM-MSCs) was investigated for its therapeutic potential against CP-induced chemobrain as well as various models of brain damage. This study was carried out to elucidate, for the first time, the role of the intravenous injection (IV) of BM-MSCs against CP-induced neurotoxicity in a rat model through investigation of the parameters of oxidative stress, inflammation, and apoptosis in brain tissue. A rat model of neurotoxicity was generated by intraperitoneal injection of 7.5 mg/kg CP while 2 × 106 BM-MSCs was given by IV as a therapeutic dose. Injection of CP led to a significant rise in malondialdehyde and nitric oxide levels accompanied by a marked depletion of superoxide dismutase and reduced glutathione content in brain tissue in comparison to the normal control (NC) rats. Furthermore, a remarkable rise in the brain levels of inflammatory cytokines interleukin (IL)-1β and IL-6, together with the expression of apoptotic marker caspase-3, and the downregulation of the brain expression of proliferating marker Ki-67 in brain tissue were detected in the CP group compared to the NC group. Histopathological alterations were observed in the brain tissue of the CP group. BM-MSCs mitigated the biochemical and histopathological alterations induced by CP without affecting brain cell proliferation. BM-MSCs could be used as a promising neuroprotective agent against CP-induced neurotoxicity.

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Changes in the Thickness of Rat Nerve Sheaths after Single Subperineural Administration of Rat Bone Marrow Mesenchymal Stem Cells

2021

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Mesenchymal stem cell therapy of brain ischemic stroke in rats

Cited by 5 publications

References 30 publications

Rat mesenchymal stem cells differentiate to endothelial cells after allotransplantation into the damaged nerve

Rat mesenchymal stem cells differentiate to endothelial cells after allotransplantation into the damaged nerve

Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy

Changes in the Thickness of Rat Nerve Sheaths after Single Subperineural Administration of Rat Bone Marrow Mesenchymal Stem Cells

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