Mesenchymal stem cells and ovarian cancer: Is there promising news?

Jahani, Saleheh; Zare, Nabi; Mirzaei, Yousef; Arefnezhad, Reza; Zarei, Hooman; Goleij, Pouya; Bagheri, Nader

doi:10.1002/jcb.30471

Cited by 4 publications

(2 citation statements)

References 101 publications

(153 reference statements)

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“…Anti-proliferative and anti-tumor activity of MVs produced by bone marrow-derived MSCs (BM-MSCs) on SKOV3 cell line and in the SCID mice model was reported [16]. It was documented that the biological/therapeutic effect of MSCs in cancer therapy depends on the tissue origin of MSCs, the application of native or genetically modified MSCs, and also on the histological type of OvCa [19,20].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell Microvesicles from Adipose Tissue: Unraveling Their Impact on Primary Ovarian Cancer Cells and Their Therapeutic Opportunities

Szyposzynska,

Bielawska-Pohl,

Murawski

et al. 2023

IJMS

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Mesenchymal stem cells (MSCs) and their derivatives can be promising tools in oncology including ovarian cancer treatment. This study aimed to determine the effect of HATMSC2-MVs (microvesicles derived from human immortalized mesenchymal stem cells of adipose tissue origin) on the fate and behavior of primary ovarian cancer cells. Human primary ovarian cancer (OvCa) cells were isolated from two sources: post-operative tissue of ovarian cancer and ascitic fluid. The phenotype of cells was characterized using flow cytometry, real-time RT-PCR, and immunofluorescence staining. The effect of HATMSC2-MVs on the biological activity of primary cells was analyzed in 2D (proliferation, migration, and cell survival) and 3D (cell survival) models. We demonstrated that HATMSC2-MVs internalized into primary ovarian cancer cells decrease the metabolic activity and induce the cancer cell death and are leading to decreased migratory activity of tumor cells. The results suggests that the anti-cancer effect of HATMSC2-MVs, with high probability, is contributed by the delivery of molecules that induce cell cycle arrest and apoptosis (p21, tumor suppressor p53, executor caspase 3) and proapoptotic regulators (bad, BIM, Fas, FasL, p27, TRAIL-R1, TRAIL-R2), and their presence has been confirmed by apoptotic protein antibody array. In this study, we demonstrate the ability to inhibit primary OvCa cells growth and apoptosis induction after exposure of OvCa cells on HATMSC2-MVs treatment; however, further studies are needed to clarify their anticancer activities.

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Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell Microvesicles from Adipose Tissue: Unraveling Their Impact on Primary Ovarian Cancer Cells and Their Therapeutic Opportunities

Szyposzynska,

Bielawska-Pohl,

Murawski

et al. 2023

IJMS

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“…Since mounting evidence has demonstrated the efficacy of different sources of stem cells [1][2][3][4] and the derived secretome [5][6][7][8], in recent years, perinatal stem cells have been considered as an alternative and available cell source for biomedical research in vitro [9,10] and in vivo [11], including clinical trials [12][13][14].…”

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confidence: 99%