Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma

Duchi, Serena; Sotgiu, Giovanna; Lucarelli, Enrico; Ballestri, Marco; Dozza, Barbara; Santi, Spartaco; Guerrini, Andrea; Dambruoso, Paolo; Giannini, Sandro; Donati, Davide María; Ferroni, Claudia; Varchi, Greta

doi:10.1016/j.jconrel.2013.03.012

Cited by 84 publications

(57 citation statements)

References 65 publications

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“…Following PDT, both control MSC and MSC+MSV failed to induce any increase in ROS signals (Figure 3c, d), whereas MSC+Ce6@MSV demonstrated a significant increase in local ROS signal, specifically within the laser’s radius (Figure 3c, white circle). Furthermore, similar to previous results, 38 we witnessed a bubble-like formation after PDT, followed by rapid cell deformation (Movie S1). To evaluate if ROS generation by Ce6@MSV is successful at triggering cell death, we used the LIVE/DEAD assay to concurrently stain both viable and dying MSC.…”

Section: Resultssupporting

confidence: 91%

Chlorin e6 Functionalized Theranostic Multistage Nanovectors Transported by Stem Cells for Effective Photodynamic Therapy

Näkki

Martinez

Evangelopoulos

et al. 2017

ACS Appl. Mater. Interfaces

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Approaches to achieve site-specific and targeted delivery that provide an effective solution to reduce adverse, off target side effects are urgently needed for cancer therapy. Here, we utilized a Trojan-horse-like strategy to carry photosensitizer Chlorin e6 conjugated porous silicon multistage nanovectors with tumor homing mesenchymal stem cells for targeted photodynamic therapy and diagnosis. The inherent versatility of multistage nanovectors permitted the conjugation of photosensitizers to enable precise cell death induction (60%) upon photodynamic therapy, while simultaneously retaining the loading capacity to load various payloads, such as antitumor drugs and diagnostic nanoparticles. Furthermore, the mesenchymal stem cells that internalized the multistage nanovectors conserved their proliferation patterns and in vitro affinity to migrate and infiltrate breast cancer cells. In vivo administration of the mesenchymal stem cells carrying photosensitizer-conjugated multistage nanovectors in mice bearing a primary breast tumor confirmed their tropism toward cancer sites exhibiting similar targeting kinetics to control cells. In addition, this approach yielded in a > 70% decrease in local tumor cell viability after in vivo photodynamic therapy. In summary, these results show the proof-of-concept of how photosensitizer conjugated multistage nanovectors transported by stem cells can target tumors and be used for effective site-specific cancer therapy while potentially minimizing potential negative side effects.

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Section: Resultssupporting

confidence: 91%

Chlorin e6 Functionalized Theranostic Multistage Nanovectors Transported by Stem Cells for Effective Photodynamic Therapy

Näkki

Martinez

Evangelopoulos

et al. 2017

ACS Appl. Mater. Interfaces

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“…More frequently, strategies rely on the passive and/or calveolin- or clathrin-assisted uptake of nanoparticles into SCs 63 . Once at the tumour site, nanoparticles are released from the SC, either as a result of membrane rupture caused by cytosolic accumulation, or by facilitating death using an external source such as photoinduction 65,66 or inducing hyperthermia 67 . Combining nanoparticle-based therapeutics with SC delivery is a nascent partnership that warrants further investigation for the treatment of cancer.…”

Section: Creating Anticancer Stem Cellsmentioning

confidence: 99%

Stem cell-based therapies for cancer treatment: separating hope from hype

2014

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Stem cell-based therapies are emerging as a promising strategy to tackle cancer. Multiple stem cell types have been shown to exhibit inherent tropism towards tumours. Moreover, when engineered to express therapeutic agents, these pathotropic delivery vehicles can effectively target sites of malignancy. This perspective considers the current status of stem cell-based treatments for cancer and provides a rationale for translating the most promising preclinical studies into the clinic.

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“…Indeed, tumors can produce a large and continuous amount of cytokines, chemokines and inflammatory mediators, which are signals capable of recruiting respondent cell types, including MSC [36, 6,37]. Our recent work on the use of MSC as drug delivery vehicles for anti-tumor therapeutic applications, has prompted us to find new approaches/techniques that can induce the release of cytotoxic ROS from MSC toward tumor cells [3].…”

Section: Discussionmentioning

confidence: 99%

“…In recent decades, various tumor-targeted therapeutic approaches have been developed at a molecular, cellular and tissue level [2]. In the last years, mesenchymal stem cells (MSC) have received much attention not only as candidates for regenerative medicine regimens, but also for their potential as "smart" delivery systems of chemotherapeutics and nanoparticles [3][4][5][6][7][8]. MSC are multipotent cells characterized by tropism toward primary and metastatic tumor locations [9,4,8].…”

Section: Introductionmentioning

confidence: 99%

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

et al. 2017

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Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell cocultures / Foglietta, Federica; Duchi, Serena; Canaparo, Roberto; Varchi, Greta; Lucarelli, Enrico; Dozza, Barbara; Serpe, Loredana. -In: LIFE SCIENCES. -ISSN 0024-3205. -173(2017), pp. 28-35. Original Citation:Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. AbstractAim: Mesenchymal stem cells (MSC) possess the distinctive feature of homing in on and engrafting into the tumor stroma making their therapeutic applications in cancer treatment very promising.Research into new effectors and external stimuli, which can selectively trigger the release of cytotoxic species from MSC toward the cancer cells, significantly raises their potential.Main methods: Shock waves (SW) have recently gained recognition for their ability to induce specific biological effects, such as the local generation of cytotoxic reactive oxygen species (ROS) in a non-invasive and tunable manner. We thus investigate whether MSC are able to generate ROS and, in turn, affect cancer cell growth when in co-colture with human glioblastoma (U87) or osteorsarcoma (U2OS) cells and exposed to SW.Key findings: MSC were found to be the cell line that was most sensitive to SW treatment as shown by SW-induced ROS production and cytotoxicity. Notably, U87 and U2OS cancer cell growth was unaffected by SW exposure. However, significant decreases in cancer cell growth, 1.8 fold for U87 and 2.3 fold for U2OS, were observed 24 h after the SW treatment of MSC co-cultures with cancer cells. The ROS production induced in MSC by SW exposure was then responsible for lipid peroxidation and cell death in U87 and U2OS cells co-cultured with MSC.Significance: This experiment highlights the unique ability of MSC to generate ROS upon SW treatment and induce the cell death of co-cultured cancer cells. SW might therefore be proposed as an innovative tool for MSC-mediated cancer treatment.3

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Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma

Cited by 84 publications

References 65 publications

Chlorin e6 Functionalized Theranostic Multistage Nanovectors Transported by Stem Cells for Effective Photodynamic Therapy

Chlorin e6 Functionalized Theranostic Multistage Nanovectors Transported by Stem Cells for Effective Photodynamic Therapy

Stem cell-based therapies for cancer treatment: separating hope from hype

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

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